A kind of method for preparing pectin disaccharide and pectin triose

A technology of pectin and low-ester pectin, which is applied in the field of sugar bioengineering, can solve the problems of low yield of pectin oligosaccharides, high viscosity of reaction system, difficult mass transfer of enzyme reaction, etc., to achieve enhanced solubility and increased content , The effect of simple process

Active Publication Date: 2021-09-28
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pectin enzymatic degradation is to use endopectinase to selectively degrade pectin to prepare pectin oligosaccharides. Enzymatic degradation has the advantages of mild reaction temperature, less by-products, and high content of pectin disaccharides and pectin trisaccharides in the degradation products. However, when pectin is directly used for enzymatic degradation, there are problems such as high viscosity of the reaction system, difficult mass transfer of the enzyme reaction, steric hindrance effect of the enzyme reaction, large amount of enzyme used, and low yield of pectin oligosaccharides.

Method used

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  • A kind of method for preparing pectin disaccharide and pectin triose

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Water heat treatment and enzymatic coupling lower ester concrete preparation of adult sugar and pectin triose, the specific steps are as follows:

[0023] (1) Weigh 1G low ester glue (less than 50%) in a 35 mL cooking tank, adding distilled water from 1: 25 (g / ml), in the oil bath pan at 140 ° C. 30min, fast cooling, centrifugation, taking the supernatant.

[0024] (2) Adjusting the supernatant to pH 5.5 obtained by dilute hydrochloric acid to pH 5.5, the inner segregation enzymes of 15, 65 and 115u / g gum are added, and the enzymatic solution is enzymatically resolved at 45 ° C. After the end, the enzyme is allowed to activate 10 min, centrifugally, and take the supernatant in boiling water. It is shown in Table 1 with high performance liquid phase ion chromatography.

[0025] Table 1 Low ester gel water heat treatment with enzymatic coupling degradation pectin diose and pectin triose yield

[0026] Endogeneous rubber enzyme dosage (U / G pectin) Pectin diose ...

Embodiment 2

[0029] The esterification degree of esterification after oligate gel is heated, and the steps are as follows:

[0030] (1) Weigh 1G low ester glue (less than 50%) in a 35 mL cooking tank, adding distilled water from 1: 25 (g / ml), in the oil bath pan at 140 ° C. 30min, quick cooling, centrifugation, taking the supernatant, dry water, and dry with the pectin sample before the water heat treatment.

[0031] (2) Take step (1) in vacuum dry water pretreatment before and after, KBR polishing tablets, using Fourier transform infrared spectrometer in 4000-400 cm -1 Scanning within the range, the results analysis figure 1 .

[0032] Depend on figure 1 It can be seen, 1751cm -1 The absorption peak at the point indicates the esterified carbonyl group, 1641cm -1 The absorption peak at the point indicates an unsterified carbonyl group, and the absorption intensity of the unsolved carbonyl is not esmified after the hydrothermal pre-treatment is significantly enhanced than the pectin before th...

Embodiment 3

[0040] 1. Water heat treatment and enzymatic coupling lower ester confection with enzymatic adhesive and pectin triose, the specific steps are as follows:

[0041] (1) Weigh 1G low ester pump (esterification degree below 50%) in a 35 ml cooking tank, add distilled water from 1:25 (g / ml), in the oil bath pan, in the oil bath, 100 ~ 180 ° C 30 min, quickly cool, centrifuge, and take the supernatant.

[0042] (2) Adjusting the supernatant to pH 5.5 by dilute hydrochloric acid to pH 5.5, the inner segregation enzyme of 15 U / G pectin is added, and the enzymatic solution is added at 45 ° C, and after the enzymatic reaction is completed, the boiling water Medium enzyme is 10 min, centrifuged, and taking the supernatant. It is shown in Table 3 with high performance liquid phase ion chromatography.

[0043] 2, hydrothermal method degradation of low ester concrete preparation of adhesive oxose and pectin trisaccharide: 1. 1G low ester pump (esterification degree below 50%) in 35ml steam...

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Abstract

The invention discloses a method for preparing pectin disaccharides and pectin trisaccharides. The high-yield pectin can be realized by coupling low-ester pectin with hydrothermal treatment at 100-140°C and selective enzymatic hydrolysis of endopectinase. Preparation of Glycobiose and Pectotriose. The test results show that the yield of pectin disaccharide and pectin trisaccharide in this method is much higher than that of hydrothermal or enzymatic degradation of low-ester pectin alone. At the same time, this method is also environmentally friendly, clean and easy to industrialize. , has good practicability.

Description

Technical field [0001] The present invention relates to the field of sugar biological engineering, and more particularly to a method of preparing curted oxose and pectin triose. Background technique [0002] Pectin is an acidic plant polysaccharide, which is widely present in higher plasma cell walls. The pectin is a neutralized glycosyl group, a neutral glycosyl group, a acetyl group such as a lactose, arabinose, a acetyl group such as alactose, arabinose, acetyl group, and the like. Polysaccharide. The carboxyl group of galacturonic acid group in the pectin molecule is often present in the form of methyl esterification, typically based on the degree of esterification of galacturonic carboxyl groups in the pectin molecule is less than 50%, and the pectin is lowered into low. Two kinds of ester esters and high-fat adhesive gels. Juice production waste (citrus skin, apple skull, etc.) contains rich pectin polysaccharides, accounting for 20-30%. A large number of juice production w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/12C12P19/00
CPCC12P19/00C12P19/12
Inventor 勇强姜美云赖晨欢黄曹兴李鑫连之娜范一民徐勇欧阳嘉余世袁
Owner NANJING FORESTRY UNIV
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