Extraction method of olive leaf polyphenol

An extraction method and technology of olive leaves, which are applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, anti-toxins, etc., to achieve the effect of improving the extraction rate

Active Publication Date: 2013-02-20
SHANGHAI CHUANGYUAN COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the extraction methods of plant polyphenols mainly include solution extraction method, ultrasonic extraction method, microwave method, etc., but the yield of a single extraction method is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Extract olive leaf polyphenols in the following steps:

[0021] (1) Rinse, dry, and pulverize the olive leaves into powder and pass through a 40-mesh sieve;

[0022] (2) Add the olive leaf powder into distilled water, the mass ratio of olive leaf powder to distilled water is 1:20, extract by microwave, set the temperature at 80°C, and set the microwave power to 120W and 160W every 20s , 200W, 240W, 280W, and then dry the extract to obtain the olive leaf polyphenol crude extract;

[0023] At this time, the extraction rate of olive leaf polyphenols was measured to be 18.2%.

[0024] (3) Add water to the crude polyphenol extract to form a 10 mg / mL solution, flow through the HP-20 type macroporous resin for adsorption, the flow rate is 3BV / h, the adsorption time is 2h, then shake in a water bath for 24h, the temperature of the water bath at 25°C, the oscillation power is 120r / min;

[0025] (4) Filter the adsorbed resin, wash it twice with distilled water, then filter dry...

Embodiment 2

[0028] Extract olive leaf polyphenols in the following steps:

[0029] (1) Rinse, dry, and pulverize the olive leaves into powder and pass through a 40-mesh sieve;

[0030] (2) Add the olive leaf powder into distilled water, the mass ratio of olive leaf powder to distilled water is 1:20, extract by microwave, set the temperature at 80°C, and set the microwave power to 150W and 200W every 40s , 250W, and then dry the extract to obtain the olive leaf polyphenol crude extract;

[0031] At this time, the extraction rate of olive leaf polyphenols was measured to be 18.9%.

[0032] (3) Add water to the crude polyphenol extract to form a 10mg / mL solution, flow through the HP-20 macroporous resin for adsorption, the flow rate is 4BV / h, the adsorption time is 3h, then shake in a water bath for 24h, the temperature of the water bath at 25°C, the oscillation power is 120r / min;

[0033] (4) Filter the adsorbed resin, wash it twice with distilled water, then filter dry, and perform dyna...

Embodiment 3

[0036] Extract olive leaf polyphenols in the following steps:

[0037] (1) Rinse, dry, and pulverize the olive leaves into powder and pass through a 40-mesh sieve;

[0038] (2) Add the olive leaf powder into distilled water, the mass ratio of olive leaf powder to distilled water is 1:20, extract by microwave, set the temperature to 80°C, and set the microwave power to 170W and 230W every 60s , and then dry the extract to obtain the olive leaf polyphenol crude extract;

[0039] At this time, the extraction rate of olive leaf polyphenols was measured to be 17.4%.

[0040] (3) Add water to the polyphenol crude extract to make a 10mg / mL solution, flow through the AB-8 type macroporous resin for adsorption, the flow rate is 4BV / h, the adsorption time is 3h, then shake in a water bath for 24h, the water bath temperature at 25°C, the oscillation power is 120r / min;

[0041] (4) Filter the adsorbed resin, wash it twice with distilled water and then dry it, then perform dynamic eluti...

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PUM

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Abstract

The invention relates to an extraction method of olive leaf polyphenol. The method comprises the following steps: with water as a solvent, obtaining olive leaf polyphenol crude extract by adopting microwave extraction, then carrying out macroporous resin separation, pressure reduction and concentration, and vacuum freeze dehydration to obtain the olive leaf polyphenol. The method is characterized in that the the microwave power is increased for every other 20-60s in the microwave extraction process. The method has the beneficial effects that the use amount of the solvent can be reduced, the extraction yield is increased, and the purity is improved.

Description

technical field [0001] The invention relates to a method for extracting olive leaf polyphenols from olive leaves, belonging to the field of plant extraction. technical background [0002] Plant polyphenols are important secondary metabolites that widely exist in plants. They are widely distributed in roots, leaves, skins, pulp, and shells of plants. They have various physiological functions such as anti-oxidation, anti-tumor, and liver protection. Antioxidant substances that must be ingested from food every day are called the seventh nutrient after dietary fiber. According to the structure, polyphenols are divided into the following groups: simple phenols (C 6 ), lignans, coumarins, stilbenes (C 6 -C 2 -C 6 ), flavonoids (C 6 -C 1 -C 6 , C 6 -C 3 -C 6 ), phenolic acids (C 6 -C 1 , C 6 -C 3 ) and tannins and other compounds. [0003] In the prior art, the extraction methods of plant polyphenols mainly include solution extraction method, ultrasonic extraction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/32A61P1/16A61P35/00A61P39/06A61K125/00
Inventor 于忠清
Owner SHANGHAI CHUANGYUAN COSMETICS
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