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Method for extracting pomegranate seed protein

A protein extraction and pomegranate seed technology, which is applied in the fields of peptide preparation, chemical instruments and methods, organic chemistry, etc., can solve the problems of waste, pomegranate seed abandonment, and resource waste.

Inactive Publication Date: 2013-02-20
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But at present, the pomegranate processing industry in Xinjiang is still at a relatively low level, mainly processing beverages. In beverage processing, a large number of pomegranate seeds are discarded as waste, resulting in a serious waste of resources.
[0004] At present, the industry's research on the deep processing and utilization of pomegranate seeds mainly stays in the extraction of oil or polyphenols. After the oil is extracted, the cake dregs still contain a lot of nutrients and are discarded as waste, resulting in waste.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Take the defatted pomegranate seed cake, add water to mix evenly according to 10 times the weight, put it into a colloid mill, grind it to below 150 μm, add NaOH to the obtained mixture to adjust the pH value to 10, let it stand for 60 minutes, and take the supernatant , use a centrifuge to centrifuge at a speed of 3500 rpm to obtain a separation liquid, add hydrochloric acid to the obtained separation liquid to adjust the pH value to 3.8, and add 20% ammonium sulfate by weight at the same time, stir evenly and let it stand for 60 minutes, then use a centrifuge The machine centrifuges at a separation speed of 3500 rpm to get the separation liquid, adjusts the pH value of the resulting solution to 7 with NaOH, and removes the sulfate component with an ultrafiltration membrane. The operating pressure difference used is 0.3 MPa, and the pressure spray drying tower Drying is carried out, the pressure of the feed reaches 5MPa, the inlet air temperature is 180°C, a...

Embodiment 2

[0021] Example 2: Take the degreased pomegranate seed cake, add water to mix evenly according to 8 times the weight, put it into a colloid mill, grind it to a thickness below 100 μm, add NaOH to the obtained mixture to adjust the pH value to 9, let it stand for 60 minutes, and take the supernatant, The resulting precipitate was leached twice, adding water to 5 times the weight of the precipitate each time and adding NaOH to adjust the pH value to 9, then standing for 60 minutes to take the supernatant, combining the obtained supernatant and centrifuging centrifuge at a speed of 3000 rpm to obtain a separation liquid, add hydrochloric acid to adjust the pH value to 4.0, add 30% ammonium sulfate by weight at the same time, stir evenly and let stand for 90 minutes, then use a centrifuge at a speed of 4000 rpm Centrifuge to get the separation liquid, adjust the pH value of the resulting solution to 7.2 with NaOH, remove the sulfate component in the protein liquid with an ultrafil...

Embodiment 3

[0022] Example 3: Take the defatted pomegranate seed cake, add water to mix evenly according to 8 times the weight, put it into a colloid mill, grind it to a thickness below 75 μm, add NaOH to the obtained mixture to adjust the pH value to 11, and let it stand for 90 minutes to take the supernatant. The resulting precipitate was leached twice, adding water to 6 times the weight of the precipitate each time and adding NaOH to adjust the pH value to 10, then standing for 60 minutes to take the supernatant, combining the obtained supernatant and centrifuging Centrifuge at a speed of 3800 rpm to obtain the separation liquid, add hydrochloric acid to adjust the pH to 3.6, add 25% ammonium sulfate by weight at the same time, stir evenly and let stand for 120 minutes, then use a centrifuge at a speed of 4000 rpm Centrifuge to get the separation liquid, adjust the pH value of the resulting solution to 7.2 with NaOH, remove the sulfate component in the protein liquid with an ultrafilt...

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PUM

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Abstract

The invention discloses a method for extracting pomegranate seed protein. The method comprises the technological steps of raw material handling, extraction, centrifugal residual removal, acid-extraction, secondary separation, neutralization, ultra-filtration, drying and the like. The method uses pomegranate seeds or pomegranate seed cakes and other seed residuals as raw materials to perform deep processing and extract the protein and turns waste into wealth.

Description

technical field [0001] The invention relates to a deep processing method of pomegranate seeds, in particular to a method for extracting pomegranate seed protein. Background technique [0002] Xinjiang is very rich in pomegranate resources, especially in areas such as Kashgar, Hotan, Aksu, and Turpan in southern Xinjiang, where pomegranates are planted on a large area. With the implementation of the country's western development strategy and the industrial adjustment and planning of the autonomous region's development of characteristic forest and fruit industries, the cultivation, deep processing and industrialization of pomegranates in southern Xinjiang have developed rapidly, and have also driven the development of related industries. [0003] However, the pomegranate processing industry in Xinjiang is still at a relatively low level, mainly processing beverages. In beverage processing, a large number of pomegranate seeds are discarded as waste, resulting in a serious waste...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 邹圣冬代绍娟程卫东
Owner SHIHEZI UNIVERSITY
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