Method for producing all-vitamin mineral oral liquid by microorganism fermentation
A microbial fermentation method, vitamin technology, applied in food science, food preparation, application, etc., to avoid antagonism and competition
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Embodiment 1
[0037] Embodiment 1: Utilize Flammulina velutipes fermentation to produce the method for the all-vitamin mineral oral liquid suitable for minors
[0038] 1. PDA slant for preservation of Flammulina velutipes strains (unit g / L): 200g potato cubes, boiled for 30 minutes, filtered to obtain juice, 40g bran, boiled for 30 minutes, filtered to obtain juice, glucose 20.0, KH 2 PO 4 1.5, MgSO 4 ·7H 2 O 0.75, agar 15.0, sterilized at 121°C for 30 minutes, made a test tube slope, inoculated with Flammulina velutipes, cultivated at 25°C for 4-5 days to make slope seeds;
[0039] 2. Preparation of Flammulina velutipes seed solution in a liquid shaker: culture medium (unit: g / L): 200g potato cubes, boiled for 30 minutes, filtered to obtain juice, 40g bran, boiled for 30 minutes, filtered to obtain juice, glucose 20.0, KH 2 PO 4 1.5, MgSO 4 ·7H 2 O 0.75, sterilized at 121°C for 30 minutes; after cooling, inoculate the seeds on the inclined plane of the test tube; at 25°C, shaker 200r...
Embodiment 2
[0053] Embodiment 2: Utilize ganoderma lucidum fermentation to produce the method for adult's whole vitamin and mineral oral liquid
[0054] 1. PDA slant for preservation of Ganoderma lucidum strains (g / L): 200g potato cubes, boiled for 30 minutes, filtered to obtain juice, 40g bran, boiled for 30 minutes, filtered to obtain juice, glucose 20.0, KH 2 PO 4 1.5, MgSO 4 ·7H 2 O 0.75, agar 15.0, sterilized at 121°C for 30 minutes, made into a test tube slant, inoculated with Ganoderma lucidum, cultivated at 28°C for 4-5 days to make slant seeds;
[0055] 2. Preparation of Ganoderma lucidum seed liquid in liquid shake bottle: Medium (g / L): 200g potato cubes, boiled for 30 minutes, filtered to obtain juice, 40g bran, boiled for 30 minutes, filtered to obtain juice, glucose 20.0, KH 2 PO 4 1.5, MgSO 4 ·7H 2 O 0.75, sterilized at 121°C for 30 minutes; after cooling, inoculate the seeds on the slant of the test tube; 30°C, shaker 180rpm, cultivate for 4 days, and obtain the culti...
Embodiment 3
[0069] Embodiment 3: Utilize Taishan Cordyceps fermentation to produce the method for adult's whole vitamin and mineral oral liquid
[0070] 1. Taishan Cordyceps strain preservation PDA slope (g / L): 200g potato cubes, boiled for 30 minutes, filtered to obtain juice, 40g bran, boiled for 30 minutes, filtered to obtain juice, glucose 20.0, KH 2 PO 4 1.5, MgSO 4 ·7H 2 O 0.75, agar 15.0, sterilized at 121°C for 30 minutes, made into a test tube slant, inoculated with Ganoderma lucidum, cultivated at 25°C for 4-5 days to make slant seeds;
[0071] 2. Preparation of Ganoderma lucidum seed liquid in liquid shake bottle: Medium (g / L): 200g potato cubes, boiled for 30 minutes, filtered to obtain juice, 40g bran, boiled for 30 minutes, filtered to obtain juice, glucose 20.0, KH 2 PO 4 1.5, MgSO 4 ·7H 2 O 0.75, sterilized at 121°C for 30 minutes; after cooling, inoculate the seeds on the slant of the test tube; at 25°C, shaker 180rpm, cultivate for 3 to 4 days to obtain the culture...
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