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Bacillus pumilus N2 and application thereof

A technology of Bacillus pumilus and strains, applied in the field of microorganisms, can solve the problems affecting the survival and reproduction of soil organisms, metabolic functions and population numbers, affecting soil element biogeochemical processes and self-purification capabilities, biomass, community structure and biodiversity Changes in properties and other issues, to achieve the effect of easy control of degradation conditions, small environmental interference factors, and few restrictive conditions

Active Publication Date: 2016-02-03
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have also shown that antibiotics entering the soil may affect the survival and reproduction, metabolic function and population size of soil organisms, etc., and change the biomass, community structure and biodiversity, thereby affecting soil element biogeochemical processes and self-purification. ability etc.
When heavy metals and antibiotics coexist in soil, soil heavy metals can drive antibiotic resistance genes and promote horizontal transfer and diffusion through co-selection, making the environmental behavior of pollutants under compound conditions more complex than those in non-complex polluted environments
[0005] Therefore, there is a lack of antibiotic-degrading bacteria that can tolerate various heavy metal environments in the prior art.

Method used

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  • Bacillus pumilus N2 and application thereof
  • Bacillus pumilus N2 and application thereof
  • Bacillus pumilus N2 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0024] Bacillus pumilus (Bacillus pumilus) N2; preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCCNO.11071. The date of deposit is July 10, 2015.

[0025] Bacillus pumilus N2 can grow in an environment with a pH value of 6-8; the suitable growth temperature is 25-35°C.

[0026] Bacillus pumilus (Bacillus pumilus) N2 strain was cultured on the beef extract peptone medium plate in a constant temperature incubator at 30°C for 2 days, and the colony was round, opaque, milky white, wrinkled on the surface, and the edges were smooth, and it was rod under the microscope shape. Physiological and biochemical tests found that the bacterial strain was a gram-positive bacterium, which could not reduce nitrate, and the citrate test was positive, and th...

Embodiment 2

[0029] In this embodiment, Bacillus pumilus N2 is isolated according to the following steps. Soil samples were collected in the mulberry field of Jiangsu University of Science and Technology. Take samples according to the 5-point sampling method, then mix all the collected soil, air-dry, and pass through a 100-mesh sieve to obtain soil samples. Then treated with 300mg / kg copper sulfate and 1000mg / kg zinc sulfate for 84 days. Weigh 1g of the soil sample and add it to 99mL of sterile saline, shake it on a shaker at 120r / min at 25°C for 2h to make soil 10 -4 The diluent is ready for use. Take the diluted solution and spread it on the plate, and incubate at 30°C for 2-4d. Described plate culture medium is basic inorganic salt (MSM) medium (g / L): K 2 HPO 4 5.8, KH 2 PO 4 4.5, (NH 4 ) 2 SO 4 2.0, MgCl 2 0.16, CaCl 2 0.02, Na 2 MoO 4 2H 2 O0.0024, FeCl 3 0.0018, MnCl 2 2H 2 O0.0015, 18g of agar, pH=7.0, sterilized at 121°C for 20min with damp heat. When sterilized ...

Embodiment 3

[0031]In this embodiment, the heavy metal-resistant antibiotic-degrading bacteria Bacillus pumilus N2 can be used to degrade antibiotics in heavy metal-polluted environments.

[0032] Dosing Bacillus pumilus N2 in an environment polluted by heavy metals and antibiotics, the environment temperature is 25-35° C., and the environment pH value is 6-8. In this embodiment, Bacillus pumilus N2 can be used for remediation of contaminated soil or water.

[0033] Select gentamicin, neomycin, streptomycin, ampicillin, penicillin sodium, cephalosporin and sulfathiazole so that the final concentration reaches 32mg / L, 32mg / L, 128mg / L, 64mg / L, 64mg / L, respectively. L, 64 and 64mg / L, Cu 2+ , Zn 2+ Concentrations are respectively 200mg / L, 100g of compound polluted soil of 400mg / L, the vesicular Brevundimonas bacillus pumilus (Bacillus pumilus) N2 that will be cultivated in the beef extract peptone liquid medium in the initial stage of stationary phase is sprayed at a ratio of 20mL / kg In the...

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Abstract

The invention provides Bacillus pumilus N2 and an application thereof. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC NO.11071 and the preservation date of July 10, 2015. The Bacillus pumilus N2 can degrade antibiotics, so that the competition among the mixed microorganisms is avoided, and the antagonism among the different soluble microbial products is avoided; meanwhile, the single microorganism material is adopted, the degradation conditions are easy to control and easy to manage. In environment repairing, the Bacillus pumilus N2 is fed as the dominant bacterial community, and the Bacillus pumilus N2 has the advantages of being slightly influenced by environmental factors, and slightly influenced by limiting conditions.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a heavy metal-resistant, antibiotic-degradable microorganism and its application in environmental restoration. Background technique [0002] Livestock and poultry organic fertilizer is widely used in agricultural production because it is rich in organic matter and nutrients such as nitrogen and phosphorus required for crop growth, as a source of fertility and improver for farmland soil. However, due to the use of a large number of heavy metal-containing feed additives and antibiotic veterinary drugs, the composition of livestock and poultry organic fertilizers has undergone qualitative changes. Due to their low bioavailability, most of heavy metals and antibiotics will be excreted with feces. After entering the farmland soil, it may bring complex pollution of heavy metals and antibiotics in the soil. [0003] Microbial degradation is considered to be the most important degrad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/07
Inventor 杨统一杨芬
Owner JIANGSU UNIV OF SCI & TECH
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