26 results about "Microbiological material" patented technology

To provide a high-hardness formulation used as a material containing microorganisms, and a method for producing the same. [Solution] The formulation according to the present disclosure comprises a solid culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured. The formulation has a water content of 1 to 15% by weight. The formulation also has a hardness of 30 to 200 N. The manufacturing method of the formulation according to the present disclosure comprises step (1): drying the culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured so that the water content is 1 to 15% by weight. The manufacturing method also comprises step (2): solidifying the dried culture.

Device (100) for collecting samples, comprising: - an elongated body (110), - at least one first collecting element (111) configured for absorbing and / or retaining an organic and / or inorganic sample, preferably a sample of biological and / or microbiological material and / or of urine, said first collecting element (111) being arranged in correspondence of said elongated body (110); - a gripping portion (122), suitable for being grasped by a user for operatively retaining the device for collecting samples (100) at least during a collection of said sample; - a first retaining element (112) for said at least one first collecting element (111), - a second retaining element (113) for said at least one first collecting element (111), said second retaining element (113) being movable with respect to said elongated body (110); wherein: - said at least one first collecting element (111) is operatively compressible and / or squeezable to allow an at least partial release of said sample, and has at least one at least partially expanded configuration (B) and a compressed and / or twisted configuration (A) and, - said second retaining element (113) is movable between at least one first position (P1) with respect to said first retaining element (112) and a second position (P2) with respect to said first retaining element (112); and wherein when said second retaining element (113) is in said first position (P1) with respect to said first retaining element (112), said first retaining element (112) and said second retaining element (113) retain said at least one first collecting element (111) in said compressed and / or twisted configuration (A), and when said second retaining element (113) is in said second position (P2) with respect to said first retaining element (112), said first retaining element (112) and said second retaining element (113) allow said at least one first collecting element (111) to assume and / or to keep said at least partially expanded configuration (B); said second retaining element (113) being configured for being coupled to, and moved by, a striker (202), preferably being part of, or coupled to, a container (200) comprising a cavity (201) configured for housing at least part of the device for collecting samples (100) and for housing and / or retaining at least part of said sample when released by said at least one first collecting element (111).

A polymeric masterbatch is provided to prepare an antimicrobial material and an antimicrobial air filter prepared with said material, which serves to filter air and reduce the growth of bacteria and fungi such as E. coli, S. aureus, Botrytis cinerea, and Aspergillus niger.

The invention relates to a pervious concrete MICP intelligent preparation and targeted reinforcement method, which comprises a big data intelligent decision-making platform, a mixing module is controlled to prepare concrete according to a mix proportion suggestion given by a digital twin system, concrete performance data is obtained through nondestructive testing, and the data is fed back to the big data intelligent decision-making platform in real time. The data is pushed to a digital twin system to establish a performance database of the microbial pervious concrete, the LMM mixed model assists the digital twin system to give an optimized microbial pervious concrete ratio, and the pervious concrete based on the MICP technology is prepared according to the optimized microbial pervious concrete ratio. When the mechanical property is obviously degraded, targeted reinforcement is carried out by utilizing microbial factors, and a reinforcement process capable of recovering the mechanical property of the pervious concrete under different environmental conditions is obtained. The MICP technology is used for fixing the microbial material to reinforce the pervious concrete, and the performance of the pervious concrete can be reinforced in a targeted mode through microorganisms in the later period.

The invention provides a method for degrading urea and EC in Baijiu by bacillus-lactic acid bacteria compound bacteria. The compound bacteria comprise Pediococcus aryabhattai DQ10 and Pediococcus acidilactici DQ42, and the preservation numbers of the Pediococcus acidilactici DQ42 and the Pediococcus acidilactici DQ42 are respectively CGMCC (China General Microbiological Culture Collection Center) NO.36245 and CGMCC NO.36386. The invention further discloses a preparation method of the compound bacteria. The two strains are separated from high-temperature Daqu of Baijiu, can tolerate high temperature, acid and ethanol, and can grow under stress conditions in the fermentation process of the Baijiu, particularly, urea and ethyl carbamate (EC) in the fermentation process of liquor products (especially the Baijiu) can be effectively degraded by singly or combining the two strains (especially the complex microbial inoculants), the content of the urea and the EC can be remarkably reduced, and the yield of the liquor products is improved. The safety of liquor products can be improved, and a novel and safe microbial material is provided for the liquor brewing industry.

The invention provides an antibacterial master batch for melt spinning spandex and a preparation method thereof.The antibacterial master batch comprises spandex resin slices, a long-carbon-chain alkyl glycidyl ether modified guanidine salt polymer anti-harmful-microorganism material, a main antioxidant, an auxiliary antioxidant, a heat stabilizer, an anti-hydrolysis agent, a lubricant and a dispersing agent. Adding the mixture into a double-screw extruder; and under the protection of inert gas, carrying out melt blending on the blended materials through a twin-screw extruder, and granulating to obtain the antibacterial master batch for melt-spinning spandex. The antibacterial master batch for melt-spun spandex is applied to antibacterial modification of melt-spun spandex fibers, the addition amount of the antibacterial master batch is 2-10% of the mass of a melt-spun spandex slice base material, and the prepared antibacterial modified melt-spun spandex fibers have the antibacterial properties of durability, high efficiency, broad spectrum, washing resistance, dissolution resistance and the like.

The invention discloses a purification treatment process for livestock breeding sewage, and belongs to the technical field of functional microbial materials and application. According to the invention, by introducing the composite functional microbial agent and the mushroom bran-based biomass composite filler, efficient removal of ammonia nitrogen, COD and other pollutants in sewage is realized, and the risk of secondary pollution caused by a traditional chemical treatment method is greatly reduced. Pseudomonas filbertii, microbacterium keratinolyticum and bacillus licheniformis in the composite functional microbial agent have a synergistic effect, so that organic matters and ammonia nitrogen in sewage can be rapidly degraded, and the composite functional microbial agent also has a good flocculation effect, has good environmental adaptability and stability, and can cope with complex and variable water quality conditions. In addition, the mushroom bran-based biomass composite filler is subjected to acid pickling activation and iron-chitosan loading modification, so that the adsorption performance and nitrogen and phosphorus removal capacity of the mushroom bran-based biomass composite filler are remarkably improved, and the deep treatment effect is further optimized. The process is simple and convenient to operate, low in operation cost and suitable for sewage treatment requirements of large-scale farms.

The present disclosure is directed to systems and methods for synthesizing a spidroin. In some embodiments, the methods comprise synthesizing a monomer in vivo in a heterologous host, the monomer comprising an N-terminus IntC domain and a C-terminus IntN domain, and post-translationally polymerizing the synthesized monomer via in vitro split-intein mediated polymerization.

The application belongs to the field of antimicrobial materials, and discloses an antibacterial bioactive coating and a preparation method thereof. The application converts β-cyclodextrin into a dialdehyde derivative through periodic acid oxidation, and copolymerizes the dialdehyde derivative with acrylated chitosan under the initiation of ultraviolet light free radicals to prepare an intelligent copolymer which can be specifically cut by β-cyclodextrinase. Natural plant polyphenols are loaded in the copolymer to realize enzyme-triggered precise release and maintain long-acting antibacterial activity. After coating, the solvent is removed, the micropores are closed, and the molecular-level secondary crosslinking is completed in one step under the treatment of supercritical CO2 environment at 35 MPa and 40 DEG C for 2 hours. Traditional high-temperature drying is not needed, internal stress and crack generation are significantly reduced, and the stability of the pores is improved. The final coating has self-repairing, high adhesion and scrub resistance, and the preparation process is environmentally friendly and efficient, and is suitable for the surface long-acting antibacterial protection of medical devices, food packaging and public facilities.

The present disclosure relates generally to materials and medical devices impregnated with antimicrobial compounds. More specifically, the materials are medical matrix materials comprising nanopores or nanochannels in which the antimicrobial compounds are disposed. In other embodiments, medical matrix materials comprises nanomaterials and antimicrobials distributed throughout the material. The materials described herein are useful for a broad spectrum of medical devices and consumer products. The present disclosure further provides methods of making the antimicrobial materials and medical devices disclosed herein.

The present invention addresses the problem of providing a microbial material that can also be applied to agricultural crop cultivation in a dry area.　The present invention provides the following endospore-forming bacteria (A) to (C) having plant-growth promoting activity in a dry environment. (A) Either Bacillus thuringiensis TTREp1 strain identified by the accession number NITE BP-04159 or Bacillus altitudinis TCo-SSREp1 strain identified by the accession number NITE BP-04160, or a mutant strain thereof (B) Paenibacillus polysaccharolyticus TP-WSREp1 strain identified by the accession number NITE BP-04163, or a mutant strain thereof (C) either Priestia aryabhattai TTREn1 strain identified by the accession number NITE BP-04165 or Priestia megaterium TC-WSREn1 strain identified by the accession number NITE BP-04166, or a mutant strain thereof

The invention provides an antibacterial master batch for melt spinning of chinlon 6 and a preparation method thereof, the antibacterial master batch comprises chinlon 6 resin slices, a long carbon chain alkyl glycidyl ether modified guanidine salt polymer anti-harmful microorganism material, a main antioxidant, an auxiliary antioxidant, a lubricant and a dispersing agent, and the preparation method comprises the following steps: uniformly mixing the components in proportion, and adding into a twin-screw extruder; and under the protection of inert gas, carrying out melt blending on the blended materials through a twin-screw extruder, and granulating to obtain the antibacterial master batch for melt spinning of chinlon 6. The antibacterial master batch for melt-spinning chinlon 6 is used for antibacterial modification of melt-spinning chinlon 6 fibers, the addition amount of the antibacterial master batch is 2-10% of the mass of a chinlon 6 resin slice base material, and the prepared antibacterial modified melt-spinning chinlon 6 has the advantages of lasting antibacterial performance, high efficiency, broad spectrum, washing resistance, dissolution resistance and the like.

The application discloses a biological dredging device and an open water composite dredging method, and belongs to the technical field of open water environment treatment. The biological dredging device comprises an aluminum alloy cuboid main frame, a fixed microorganism container coated with a fine steel wire mesh is arranged in the frame, and a fixed microorganism material with a carrier such as a volcanic rock and a membrane-hung aerobic bacteria is arranged in the frame; a float is hung on both sides of the frame, an aeration fan is arranged in the frame and is connected with an aeration pipe with a micro-nano aeration head, a push flow driving module and a solar power supply module can be additionally arranged; according to the composite dredging method, after the initial measurement of a silt layer, biological dredging is performed to a preset switching depth by using the biological dredging device, then a mechanical dredging device is selected according to water body parameters to dig to a design depth, and the design depth is confirmed by subtracting the biological dredging amount from the total dredging amount. The application is suitable for the dredging of open water bodies such as rivers, lakes, aquaculture tail water areas and the like, and the dredging efficiency is significantly improved and the cost is reduced through the complementary advantages of biological dredging and mechanical dredging in time and space.

The present invention addresses the problem of providing a bacterial strain having exceptional nitrogen-fixing capabilities and N2O-reducing capabilities, and a microbial material including said bacterial strain.　The problem can be solved by a microbial material including a bacterial strain belonging to the Bradyrhizobium ottawaense clade that has nitrogen-fixing capabilities and N2O-reducing capabilities.

The invention provides a restoration and treatment method for desertification land in an alpine region, and belongs to the technical field of ecological restoration of alpine degraded land. The desertification land restoration method comprises the steps that after an environment-friendly chemical sand fixing agent with the anti-freezing performance is sprayed to the surface layer of the desertification land, dominant cyanobacteria liquid in the alpine region is evenly sprayed to the surface of a mixture of sandy soil and the sand fixing agent, and green, environment-friendly and continuous restoration of the desertification land in the alpine region is achieved. According to the method, a chemical and microbial material combined repair method is adopted, the surface stability of the sandy soil is improved by using an environment-friendly chemical sand fixing agent with anti-freezing performance, the colonization of artificial algae crust is promoted, and the sand fixing method and technology of the desertified land are enriched; through colonization growth time including eight months in complete winter, the thickness of the artificial algal crust, the chlorophyll content and the relative abundance of cyanobacteria phylum are all remarkably improved, the viability of the artificial colonization algal crust is improved, operation and construction are easy, and the later maintenance cost is low.

The application discloses carbon nanodots for resisting multi-drug resistant bacterial biofilm and a preparation method and application thereof. The carbon nanodots are prepared as follows: taking dopamine hydrochloride and diethylene triamine as raw materials, dissolving in deionized water, transferring the reaction solution to a reaction kettle, reacting at 80-200 DEG C for 1-24h, and subjecting the reaction product to dialysis and freeze-drying to obtain the carbon nanodots. The prepared carbon nanodots are used for resisting multi-drug resistant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) biofilm. The results show that the carbon nanodots have excellent performance of inhibiting the formation of MRSA biofilm and eliminating mature MRSA biofilm. In addition, the carbon nanodots can be applied to the preservation of perishable foods such as strawberries. The preparation method is simple and easy to operate, has low cost and good reproducibility, provides entities and technical guidance for the prevention and treatment of biofilm related infections and food safety protection, and provides a basis for the preparation and application of multifunctional antimicrobial materials.

This invention discloses a purification process for livestock farm wastewater, belonging to the field of functional microbial materials and application technology. By introducing a composite functional bacterial agent and a biomass composite filler based on bacterial bran, this invention achieves highly efficient removal of ammonia nitrogen, COD, and other pollutants from wastewater, while significantly reducing the risk of secondary pollution from traditional chemical treatment methods. The composite functional bacterial agent, containing *Pseudomonas hazelnutans*, *Microbacterium keratolyticum*, and *Bacillus licheniformis*, works synergistically to rapidly degrade organic matter in wastewater, while also exhibiting good flocculation effects on ammonia nitrogen. It possesses excellent environmental adaptability and stability, capable of handling complex and changing water quality conditions. Furthermore, the biomass composite filler based on bacterial bran, through acid washing activation and iron-chitosan loading modification, significantly improves its adsorption performance and nitrogen and phosphorus removal capabilities, further optimizing the deep treatment effect. This process is simple to operate, has low operating costs, and is suitable for the wastewater treatment needs of large-scale livestock farms.

Materials with uniform electrostatic surface charge for antimicrobial pathogen inactivation while maintaining safety for use by individuals and workers (no cytotoxicity), as well as methods for making such antimicrobial materials and uses thereof.

The application provides a Rhodopseudomonas palustris SX2 and an application thereof, an iron oxidation method, a composition and a culture, and belongs to the technical field of environmental microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 38191. The strain can efficiently oxidize free state and organic combined state Fe(II) under anaerobic light conditions, especially has stronger oxidation capacity for the organic combined state Fe(II), and the oxidation process is coupled with cell growth and inorganic carbon fixation; the photo-iron oxidation product is goethite short-range ordered nanocrystalline state iron mineral, and the iron minerals formed under different iron forms are different in spatial distribution and particle size. The Rhodopseudomonas palustris SX2 provided by the application provides a microbial material with excellent performance for environmental remediation, iron resource, sewage treatment and divalent iron pollution treatment.

The invention discloses an azotobacter composition and application thereof in enhancing sugarcane resistance, and belongs to the technical field of microorganisms. The method comprises the following steps: respectively carrying out fermentation culture on Azotobacter salinistris, Delftia tsuura and Vibrio natriegens, carrying out centrifugal resuspension, and mixing the obtained bacterial solutions, so as to prepare the composite bacterial agent capable of enhancing the resistance of the sugarcane. The method comprises the following steps: carrying out fermentation culture on Azotobacter salinistris, Delftia tsuura and Vibrio natriegens, and carrying out centrifugal resuspension on the obtained bacterial solutions so as to obtain the composite bacterial agent capable of enhancing the resistance of the sugarcane. When the complex microbial inoculant is applied to the sugarcane, the experimental result shows that the complex microbial inoculant can effectively enhance the resistance of the sugarcane to bacterial diseases and drought stress, and the three nitrogen-fixing bacteria have a remarkable synergistic interaction effect. The invention provides a novel microbial material and a treatment method for improving drought resistance and disease resistance of sugarcane in sugarcane planting, and the method is simple in step, low in cost, green and environment-friendly, and has outstanding application value and wide application prospect.

The invention discloses a biocontrol inoculant, a preparation method and application of the biocontrol inoculant in prevention and treatment of root rot of salvia miltiorrhiza, and belongs to the technical field of microbial materials. The biocontrol inoculant comprises a solid inoculant, a liquid inoculant and a microbial activity accelerant; the solid microbial inoculum is prepared by carrying out solid state fermentation on trichoderma harzianum CGMCC 3.10154; the liquid fungicide is prepared by carrying out liquid state fermentation on bacillus pumilus CGMCC (China General Microbiological Culture Collection Center) 1.9055; the microbial activity accelerant is prepared from L-glutamine, mannitol and sodium molybdate according to the mass ratio of (1 to 3): (2 to 5): (0.2 to 0.5). Through the synergistic effect of the functional strain and the accelerant, the microbial inoculum effectively solves the problems that an existing biocontrol microbial inoculum is weak in colonization ability and unstable in control effect, remarkably promotes the growth of salvia miltiorrhiza, improves the yield and the content of active ingredients such as salvianolic acid B, achieves the comprehensive effects of disease prevention, growth promotion and quality improvement, and is wide in application prospect.

A puck is made of a porous resilient material and is held in a housing to comprise a cable swipe cassette. The puck holds fluid in the porous material, and there is a bore through the puck. A cable is routed through the bore so that when the cable is pulled through the bore the exterior surface of the cable is wetted with the fluid. The fluid can have an antimicrobial effect so as to disinfect the cable.

The application discloses a biocontrol agent, a preparation method and application of the biocontrol agent in prevention and treatment of root rot of Salvia miltiorrhiza, and belongs to the technical field of microbial materials. The biocontrol agent comprises a solid bacterial agent, a liquid bacterial agent and a microbial activity promoter; the solid bacterial agent is prepared by solid-state fermentation of Trichoderma harzianum CGMCC 3.10154; the liquid bacterial agent is prepared by liquid-state fermentation of Bacillus lentimorbus CGMCC 1.9055; and the microbial activity promoter is composed of L-glutamine, mannitol and sodium molybdate in a mass ratio of (1-3):(2-5):(0.2-0.5). The bacterial agent of the application effectively solves the problems of weak colonization ability and unstable prevention effect of the existing biocontrol agent through the synergistic effect of functional strains and the promoter, significantly promotes the growth of Salvia miltiorrhiza, improves the yield and the content of active ingredients such as salvianolic acid B, realizes the comprehensive effects of disease prevention, growth promotion and quality improvement, and has a wide application prospect.

The application discloses a novel high-efficiency flocculation denitrifying bacteria and application thereof. The bacteria is Thauera sp. JM12B12, which is separated and screened from water in a South American white prawn breeding pond, and is preserved in the Guangdong Microbial Digital Culture Collection Center (GDMCC) on June 27, 2023, and the address is No. 59 Building, Yard, 100, Middle Martyrs Road, Yuexiu District, Guangzhou City, Guangdong Province, and the preservation number is GDMCC No. 63587. The Thauera sp. JM12B12 of the application is separated from water in a South American white prawn breeding pond, that is, the removal rates of nitrate nitrogen and nitrite nitrogen of the strain are both 100% when the carbon-nitrogen ratio is 5-20. In addition, the strain also has a high-efficiency flocculation function, that is, the flocculation efficiencies of the bacterial cells and supernatant are 90.6% and 90.1% respectively when nitrate nitrogen is removed. The novel high-efficiency flocculation denitrifying bacteria JM12B12 disclosed by the application has important application value in removal of denitrification and solid suspended matter of wastewater with excessive nitrogen content, and provides a novel microbial material for water environment protection.

The invention provides a human mycoplasma pneumoniae culture medium, a mycoplasma culture method and application, and relates to the technical field of microbial materials. The human mycoplasma pneumoniae culture medium comprises a basic culture medium, fetal calf serum, ampicillin sodium and an additive, and the additive comprises the following components based on the total volume of the human mycoplasma pneumoniae culture medium: 0.8-1.6 mg / L of glutamic acid, 0.4-1.2 mg / L of cysteine, 0.6-1.4 mg / L of glutathione, 50-150 mM / L of sodium pyruvate and 50-200 mL / L of a yeast extract. And the pH value of the human mycoplasma pneumoniae culture medium after sterilization is 7.6-8.0. According to the invention, the growth and reproduction speed of the mycoplasma pneumoniae is accelerated through the synergistic effect of the specific additive composition and content and the whole culture medium, a large number of mycoplasma pneumoniae wet thalli can be obtained in a short time, the survival rate of the human mycoplasma pneumoniae is obviously improved, and the method can be widely applied to large-scale production of the human mycoplasma pneumoniae natural antigen.