42 results about "Microbiological material" patented technology

To provide a high-hardness formulation used as a material containing microorganisms, and a method for producing the same. [Solution] The formulation according to the present disclosure comprises a solid culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured. The formulation has a water content of 1 to 15% by weight. The formulation also has a hardness of 30 to 200 N. The manufacturing method of the formulation according to the present disclosure comprises step (1): drying the culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured so that the water content is 1 to 15% by weight. The manufacturing method also comprises step (2): solidifying the dried culture.

The invention provides a platinum adsorption microbial material and a preparation method and application thereof, and belongs to the technical field of precious metal adsorption. The preparation method of the platinum adsorption microbial material comprises the following steps: (1) inoculating shewanella and escherichia coli with metal binding protein genes displayed on the surface into a fermentation culture medium for culture, and carrying out solid-liquid separation to obtain a fermentation inoculant; and (2) fixing the fermentation inoculant obtained in the step (1) on an immobilized carrier to obtain the platinum adsorption microbial material. The platinum adsorption microbial material prepared by the invention not only has the capability of efficiently adsorbing platinum, but also is not easily interfered by other metal ions, and has the effect of selectively adsorbing metal platinum. The platinum adsorption microbial material prepared by the invention is particularly suitable for adsorbing platinum in industrial wastewater, and has important significance on recycling of noble metal resources.

Device (100) for collecting samples, comprising: - an elongated body (110), - at least one first collecting element (111) configured for absorbing and / or retaining an organic and / or inorganic sample, preferably a sample of biological and / or microbiological material and / or of urine, said first collecting element (111) being arranged in correspondence of said elongated body (110); - a gripping portion (122), suitable for being grasped by a user for operatively retaining the device for collecting samples (100) at least during a collection of said sample; - a first retaining element (112) for said at least one first collecting element (111), - a second retaining element (113) for said at least one first collecting element (111), said second retaining element (113) being movable with respect to said elongated body (110); wherein: - said at least one first collecting element (111) is operatively compressible and / or squeezable to allow an at least partial release of said sample, and has at least one at least partially expanded configuration (B) and a compressed and / or twisted configuration (A) and, - said second retaining element (113) is movable between at least one first position (P1) with respect to said first retaining element (112) and a second position (P2) with respect to said first retaining element (112); and wherein when said second retaining element (113) is in said first position (P1) with respect to said first retaining element (112), said first retaining element (112) and said second retaining element (113) retain said at least one first collecting element (111) in said compressed and / or twisted configuration (A), and when said second retaining element (113) is in said second position (P2) with respect to said first retaining element (112), said first retaining element (112) and said second retaining element (113) allow said at least one first collecting element (111) to assume and / or to keep said at least partially expanded configuration (B); said second retaining element (113) being configured for being coupled to, and moved by, a striker (202), preferably being part of, or coupled to, a container (200) comprising a cavity (201) configured for housing at least part of the device for collecting samples (100) and for housing and / or retaining at least part of said sample when released by said at least one first collecting element (111).

A polymeric masterbatch is provided to prepare an antimicrobial material and an antimicrobial air filter prepared with said material, which serves to filter air and reduce the growth of bacteria and fungi such as E. coli, S. aureus, Botrytis cinerea, and Aspergillus niger.

The invention relates to a pervious concrete MICP intelligent preparation and targeted reinforcement method, which comprises a big data intelligent decision-making platform, a mixing module is controlled to prepare concrete according to a mix proportion suggestion given by a digital twin system, concrete performance data is obtained through nondestructive testing, and the data is fed back to the big data intelligent decision-making platform in real time. The data is pushed to a digital twin system to establish a performance database of the microbial pervious concrete, the LMM mixed model assists the digital twin system to give an optimized microbial pervious concrete ratio, and the pervious concrete based on the MICP technology is prepared according to the optimized microbial pervious concrete ratio. When the mechanical property is obviously degraded, targeted reinforcement is carried out by utilizing microbial factors, and a reinforcement process capable of recovering the mechanical property of the pervious concrete under different environmental conditions is obtained. The MICP technology is used for fixing the microbial material to reinforce the pervious concrete, and the performance of the pervious concrete can be reinforced in a targeted mode through microorganisms in the later period.

The present invention discloses a 3D microbial printed body and its preparation method and application. The preparation method of the 3D microbial printed body includes: step 1, using dual-network biological ink to perform vertical alternating 3D printing, fixing different microorganisms with quorum sensing effects in different printing layers to obtain a 3D microbial printed body; step 2, pre-crosslinking the 3D microbial printed body and post-crosslinking in a crosslinking solution to obtain a structurally stable 3D microbial printed body. The present invention uses a dual-network printing ink with high affinity and high mechanical properties to manufacture highly functional 3D microbial materials, so that it can ensure the high metabolic activity of bacteria in a pre-designed structure, and fix microorganisms with quorum sensing benefits in different areas by designing a specific structure, thereby improving the efficiency of bioremediation while having resistance to complex environmental impacts.

The invention provides a method for degrading urea and EC in Baijiu by bacillus-lactic acid bacteria compound bacteria. The compound bacteria comprise Pediococcus aryabhattai DQ10 and Pediococcus acidilactici DQ42, and the preservation numbers of the Pediococcus acidilactici DQ42 and the Pediococcus acidilactici DQ42 are respectively CGMCC (China General Microbiological Culture Collection Center) NO.36245 and CGMCC NO.36386. The invention further discloses a preparation method of the compound bacteria. The two strains are separated from high-temperature Daqu of Baijiu, can tolerate high temperature, acid and ethanol, and can grow under stress conditions in the fermentation process of the Baijiu, particularly, urea and ethyl carbamate (EC) in the fermentation process of liquor products (especially the Baijiu) can be effectively degraded by singly or combining the two strains (especially the complex microbial inoculants), the content of the urea and the EC can be remarkably reduced, and the yield of the liquor products is improved. The safety of liquor products can be improved, and a novel and safe microbial material is provided for the liquor brewing industry.

The invention provides an antibacterial master batch for melt spinning spandex and a preparation method thereof.The antibacterial master batch comprises spandex resin slices, a long-carbon-chain alkyl glycidyl ether modified guanidine salt polymer anti-harmful-microorganism material, a main antioxidant, an auxiliary antioxidant, a heat stabilizer, an anti-hydrolysis agent, a lubricant and a dispersing agent. Adding the mixture into a double-screw extruder; and under the protection of inert gas, carrying out melt blending on the blended materials through a twin-screw extruder, and granulating to obtain the antibacterial master batch for melt-spinning spandex. The antibacterial master batch for melt-spun spandex is applied to antibacterial modification of melt-spun spandex fibers, the addition amount of the antibacterial master batch is 2-10% of the mass of a melt-spun spandex slice base material, and the prepared antibacterial modified melt-spun spandex fibers have the antibacterial properties of durability, high efficiency, broad spectrum, washing resistance, dissolution resistance and the like.

The invention discloses a purification treatment process for livestock breeding sewage, and belongs to the technical field of functional microbial materials and application. According to the invention, by introducing the composite functional microbial agent and the mushroom bran-based biomass composite filler, efficient removal of ammonia nitrogen, COD and other pollutants in sewage is realized, and the risk of secondary pollution caused by a traditional chemical treatment method is greatly reduced. Pseudomonas filbertii, microbacterium keratinolyticum and bacillus licheniformis in the composite functional microbial agent have a synergistic effect, so that organic matters and ammonia nitrogen in sewage can be rapidly degraded, and the composite functional microbial agent also has a good flocculation effect, has good environmental adaptability and stability, and can cope with complex and variable water quality conditions. In addition, the mushroom bran-based biomass composite filler is subjected to acid pickling activation and iron-chitosan loading modification, so that the adsorption performance and nitrogen and phosphorus removal capacity of the mushroom bran-based biomass composite filler are remarkably improved, and the deep treatment effect is further optimized. The process is simple and convenient to operate, low in operation cost and suitable for sewage treatment requirements of large-scale farms.

The purpose of the present disclosure is to provide an agricultural composition, a seed composition, a method for promoting plant growth, and a method for soil improvement that are capable of improving plant growth and that use a combination of a biosurfactant and a microbial inoculant.　One embodiment of the present invention is an agricultural composition containing a biosurfactant and a microbial inoculant.

The present disclosure provides functional fibrous material comprising fibers associated with hydrogel containing micro-flakes. The present disclosure also provides a method of forming hydrogel micro-flakes having embedded therein at least one microorganism and a method of preparing functional fibrous material, both methods comprise a step of subjecting a mixture of hydrogel and microbial material to high shear forces to form micro-flakes comprising the hydrogel with at least one microorganism embedded therein. Further provided is a method of treatment of a target comprising contacting the target with the disclosed functional fibrous material as well as the micro-flakes disclosed herein.

The present disclosure is directed to systems and methods for synthesizing a spidroin. In some embodiments, the methods comprise synthesizing a monomer in vivo in a heterologous host, the monomer comprising an N-terminus IntC domain and a C-terminus IntN domain, and post-translationally polymerizing the synthesized monomer via in vitro split-intein mediated polymerization.

The application belongs to the field of antimicrobial materials, and discloses an antibacterial bioactive coating and a preparation method thereof. The application converts β-cyclodextrin into a dialdehyde derivative through periodic acid oxidation, and copolymerizes the dialdehyde derivative with acrylated chitosan under the initiation of ultraviolet light free radicals to prepare an intelligent copolymer which can be specifically cut by β-cyclodextrinase. Natural plant polyphenols are loaded in the copolymer to realize enzyme-triggered precise release and maintain long-acting antibacterial activity. After coating, the solvent is removed, the micropores are closed, and the molecular-level secondary crosslinking is completed in one step under the treatment of supercritical CO2 environment at 35 MPa and 40 DEG C for 2 hours. Traditional high-temperature drying is not needed, internal stress and crack generation are significantly reduced, and the stability of the pores is improved. The final coating has self-repairing, high adhesion and scrub resistance, and the preparation process is environmentally friendly and efficient, and is suitable for the surface long-acting antibacterial protection of medical devices, food packaging and public facilities.

The present disclosure relates generally to materials and medical devices impregnated with antimicrobial compounds. More specifically, the materials are medical matrix materials comprising nanopores or nanochannels in which the antimicrobial compounds are disposed. In other embodiments, medical matrix materials comprises nanomaterials and antimicrobials distributed throughout the material. The materials described herein are useful for a broad spectrum of medical devices and consumer products. The present disclosure further provides methods of making the antimicrobial materials and medical devices disclosed herein.

The invention discloses a method for preparing high-solubility walnut protein powder through enzymolysis, and belongs to the technical field of microbial materials. The invention provides a novel method for preparing high-solubility walnut protein powder. The novel method comprises the steps of multi-step enzymolysis, microbiological post-treatment, desalination, debitterization, purification, concentration, drying and the like. Compared with the traditional method, the walnut protein powder prepared by the method disclosed by the invention is remarkably improved in the aspects of solubility, flavor, stability, nutritional value and the like, and has a wide market application prospect. In the food industry, the protein additive can be used as a high-quality protein additive to be applied to products such as various drinks, dairy products, baked foods and the like, and the nutritional quality and the taste of the products are improved. In the field of health care products, the walnut protein powder with high solubility can be better absorbed and utilized by human bodies, and more effective nutritional supplement is provided for consumers.

The present invention relates to microorganisms, materials and methods for improving the activity of microorganisms. In particular, the present invention provides methods for preparing microorganisms to improve metabolic activity and / or synergy, metabolism-activated and / or synergistic microorganisms, and methods of using these metabolism-activated and / or synergistic microorganisms, particularly in industrial fermentation processes.

The present disclosure relates generally to materials and medical devices impregnated with antimicrobial compounds. More specifically, the materials are medical matrix materials comprising nanopores or nanochannels in which the antimicrobial compounds are disposed. In other embodiments, medical matrix materials comprises nanomaterials and antimicrobials distributed throughout the material. The materials described herein are useful for a broad spectrum of medical devices and consumer products. The present disclosure further provides methods of making the antimicrobial materials and medical devices disclosed herein.

The present invention addresses the problem of providing a microbial material that can also be applied to agricultural crop cultivation in a dry area.　The present invention provides the following endospore-forming bacteria (A) to (C) having plant-growth promoting activity in a dry environment. (A) Either Bacillus thuringiensis TTREp1 strain identified by the accession number NITE BP-04159 or Bacillus altitudinis TCo-SSREp1 strain identified by the accession number NITE BP-04160, or a mutant strain thereof (B) Paenibacillus polysaccharolyticus TP-WSREp1 strain identified by the accession number NITE BP-04163, or a mutant strain thereof (C) either Priestia aryabhattai TTREn1 strain identified by the accession number NITE BP-04165 or Priestia megaterium TC-WSREn1 strain identified by the accession number NITE BP-04166, or a mutant strain thereof

The invention provides an antibacterial master batch for melt spinning of chinlon 6 and a preparation method thereof, the antibacterial master batch comprises chinlon 6 resin slices, a long carbon chain alkyl glycidyl ether modified guanidine salt polymer anti-harmful microorganism material, a main antioxidant, an auxiliary antioxidant, a lubricant and a dispersing agent, and the preparation method comprises the following steps: uniformly mixing the components in proportion, and adding into a twin-screw extruder; and under the protection of inert gas, carrying out melt blending on the blended materials through a twin-screw extruder, and granulating to obtain the antibacterial master batch for melt spinning of chinlon 6. The antibacterial master batch for melt-spinning chinlon 6 is used for antibacterial modification of melt-spinning chinlon 6 fibers, the addition amount of the antibacterial master batch is 2-10% of the mass of a chinlon 6 resin slice base material, and the prepared antibacterial modified melt-spinning chinlon 6 has the advantages of lasting antibacterial performance, high efficiency, broad spectrum, washing resistance, dissolution resistance and the like.

The invention discloses a method for eliminating Cd in soil through microorganism construction, and belongs to the field of microorganisms. The invention screens out a microorganism for eliminating Cd pollution, namely Arthrobacter sp. SC2-19, the tolerant CdCl2 concentration of the Arthrobacter sp. SC2-19 is up to 2800mg / L, and the Arthrobacter sp. SC2-19 has a good adsorption characteristic on heavy metal cadmium and is an excellent microorganism material for heavy metal cadmium pollution treatment. After the arthrobacter SC2-19 strain is inoculated into soil, functional groups on the surfaces of thalli can quickly adsorb heavy metal ions on the surfaces of the thalli, the content of exchangeable cadmium in the soil is reduced, the content of reducible cadmium in the soil and the content of residual cadmium in the soil are increased, and when the concentration of Cd < 2 + > is 5 mg / kg, the content of the exchangeable cadmium in the soil can be reduced from 0.6226 mg / kg to 0.175 mg / kg within 120 days.

The application discloses a biological dredging device and an open water composite dredging method, and belongs to the technical field of open water environment treatment. The biological dredging device comprises an aluminum alloy cuboid main frame, a fixed microorganism container coated with a fine steel wire mesh is arranged in the frame, and a fixed microorganism material with a carrier such as a volcanic rock and a membrane-hung aerobic bacteria is arranged in the frame; a float is hung on both sides of the frame, an aeration fan is arranged in the frame and is connected with an aeration pipe with a micro-nano aeration head, a push flow driving module and a solar power supply module can be additionally arranged; according to the composite dredging method, after the initial measurement of a silt layer, biological dredging is performed to a preset switching depth by using the biological dredging device, then a mechanical dredging device is selected according to water body parameters to dig to a design depth, and the design depth is confirmed by subtracting the biological dredging amount from the total dredging amount. The application is suitable for the dredging of open water bodies such as rivers, lakes, aquaculture tail water areas and the like, and the dredging efficiency is significantly improved and the cost is reduced through the complementary advantages of biological dredging and mechanical dredging in time and space.

The invention discloses compound bacteria for sheep manure fermentation and a method for preparing an organic fertilizer through fermentation, and belongs to the technical field of microbial materials. The compound bacteria comprise functional bacteria I, functional bacteria II, a compound porous carrier and a microbial activity accelerant. The compound bacteria comprise specific functional bacteria, a compound porous carrier and a microbial activity accelerant, and all the components have a synergistic effect, so that the fermentation of the sheep manure can be effectively promoted, and harmful substances such as antibiotics possibly existing in the sheep manure can be better treated. The compound bacteria are used for fermenting sheep manure to prepare the organic fertilizer, so that the composting time is expected to be shortened, the degradation degree of harmful substances in the manure is improved, the pollution to the environment is reduced, meanwhile, high-quality organic fertilizer is provided for agricultural production, and the sustainable development of livestock breeding industry and agriculture is promoted.

The present invention addresses the problem of providing a bacterial strain having exceptional nitrogen-fixing capabilities and N2O-reducing capabilities, and a microbial material including said bacterial strain.　The problem can be solved by a microbial material including a bacterial strain belonging to the Bradyrhizobium ottawaense clade that has nitrogen-fixing capabilities and N2O-reducing capabilities.

The invention provides a restoration and treatment method for desertification land in an alpine region, and belongs to the technical field of ecological restoration of alpine degraded land. The desertification land restoration method comprises the steps that after an environment-friendly chemical sand fixing agent with the anti-freezing performance is sprayed to the surface layer of the desertification land, dominant cyanobacteria liquid in the alpine region is evenly sprayed to the surface of a mixture of sandy soil and the sand fixing agent, and green, environment-friendly and continuous restoration of the desertification land in the alpine region is achieved. According to the method, a chemical and microbial material combined repair method is adopted, the surface stability of the sandy soil is improved by using an environment-friendly chemical sand fixing agent with anti-freezing performance, the colonization of artificial algae crust is promoted, and the sand fixing method and technology of the desertified land are enriched; through colonization growth time including eight months in complete winter, the thickness of the artificial algal crust, the chlorophyll content and the relative abundance of cyanobacteria phylum are all remarkably improved, the viability of the artificial colonization algal crust is improved, operation and construction are easy, and the later maintenance cost is low.

The application discloses carbon nanodots for resisting multi-drug resistant bacterial biofilm and a preparation method and application thereof. The carbon nanodots are prepared as follows: taking dopamine hydrochloride and diethylene triamine as raw materials, dissolving in deionized water, transferring the reaction solution to a reaction kettle, reacting at 80-200 DEG C for 1-24h, and subjecting the reaction product to dialysis and freeze-drying to obtain the carbon nanodots. The prepared carbon nanodots are used for resisting multi-drug resistant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) biofilm. The results show that the carbon nanodots have excellent performance of inhibiting the formation of MRSA biofilm and eliminating mature MRSA biofilm. In addition, the carbon nanodots can be applied to the preservation of perishable foods such as strawberries. The preparation method is simple and easy to operate, has low cost and good reproducibility, provides entities and technical guidance for the prevention and treatment of biofilm related infections and food safety protection, and provides a basis for the preparation and application of multifunctional antimicrobial materials.

This invention discloses a purification process for livestock farm wastewater, belonging to the field of functional microbial materials and application technology. By introducing a composite functional bacterial agent and a biomass composite filler based on bacterial bran, this invention achieves highly efficient removal of ammonia nitrogen, COD, and other pollutants from wastewater, while significantly reducing the risk of secondary pollution from traditional chemical treatment methods. The composite functional bacterial agent, containing *Pseudomonas hazelnutans*, *Microbacterium keratolyticum*, and *Bacillus licheniformis*, works synergistically to rapidly degrade organic matter in wastewater, while also exhibiting good flocculation effects on ammonia nitrogen. It possesses excellent environmental adaptability and stability, capable of handling complex and changing water quality conditions. Furthermore, the biomass composite filler based on bacterial bran, through acid washing activation and iron-chitosan loading modification, significantly improves its adsorption performance and nitrogen and phosphorus removal capabilities, further optimizing the deep treatment effect. This process is simple to operate, has low operating costs, and is suitable for the wastewater treatment needs of large-scale livestock farms.

Materials with uniform electrostatic surface charge for antimicrobial pathogen inactivation while maintaining safety for use by individuals and workers (no cytotoxicity), as well as methods for making such antimicrobial materials and uses thereof.

The application provides a Rhodopseudomonas palustris SX2 and an application thereof, an iron oxidation method, a composition and a culture, and belongs to the technical field of environmental microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 38191. The strain can efficiently oxidize free state and organic combined state Fe(II) under anaerobic light conditions, especially has stronger oxidation capacity for the organic combined state Fe(II), and the oxidation process is coupled with cell growth and inorganic carbon fixation; the photo-iron oxidation product is goethite short-range ordered nanocrystalline state iron mineral, and the iron minerals formed under different iron forms are different in spatial distribution and particle size. The Rhodopseudomonas palustris SX2 provided by the application provides a microbial material with excellent performance for environmental remediation, iron resource, sewage treatment and divalent iron pollution treatment.

[Problem] To provide a system and a method for extracting valuable matter from a biomass raw material. [Solution] The present invention comprises: a culture device 301 for culturing microorganisms 201; a concentration device 302 for concentrating the cultured microorganisms 201; a solubilization treatment device 303 that subjects concentrated microorganisms 201A which have been concentrated to a hydrothermal solubilization treatment; a filtration device 304 that filters a solubilization treated liquid 211 from the solubilization treatment device 303; a drying device 305 that dries a microorganism filtered product 201B from the filtration device 304 to obtain a microorganism dried product 201C; and an extraction device 306 that extracts, in an extraction solvent 215, a valuable component in the microorganism dried product 201C.