Purification method of ganirelix acetate
A technique of ganirelix and a purification method, which is applied in the field of medicinal chemistry, can solve problems such as inhomogeneous salt formation of refined peptides, toxicity of trifluoroacetic acid, unfavorable purification, etc., and achieve good elution peak shape, improved purification scale, and easy The effect of industrial production
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Embodiment 1
[0029] Embodiment 1: the purification of ganirelix acetate
[0030] (1) Purification of ganirelix crude peptide:
[0031] Dissolve 2.0 g of crude ganirelix peptide in 100 ml of purified water and acetonitrile (v:v=3:1), filter, and collect the filtrate for later use.
[0032] Purification chromatography conditions:
[0033] Chromatographic column: 50×250mm, filled with octadecylsilane bonded silica gel as stationary phase filler.
[0034] Flow rate: 80ml / min.
[0035] Monitoring wavelength: 280nm.
[0036]Mobile phase A phase: 20mM sodium perchlorate solution, adjust pH to 1.5 with phosphoric acid.
[0037] Mobile Phase B: Acetonitrile.
[0038] gradient:
[0039] Sample loading: 2.0g (100ml).
[0040] After equilibrating the chromatographic column with 75%A+25%B for 5 minutes, load the sample, run gradient purification, monitor and collect the target peak fractions in three sections: before the peak, at the top of the peak, and after the peak. The pre-peak and post-...
Embodiment 2
[0052] Embodiment 2: the purification of ganirelix acetate
[0053] (1) Purification of ganirelix crude peptide:
[0054] Dissolve 2.0 g of crude ganirelix peptide in 100 ml of purified water and acetonitrile (v:v=3:1), filter, and collect the filtrate for later use.
[0055] Purification chromatography conditions:
[0056] Chromatographic column: 50×250mm, filled with octadecylsilane bonded silica gel as stationary phase filler.
[0057] Flow rate: 80ml / min.
[0058] Monitoring wavelength: 280nm.
[0059] Mobile phase A phase: 50mM sodium perchlorate solution, adjust the pH to 2.0 with phosphoric acid.
[0060] Mobile Phase B: Acetonitrile.
[0061] gradient:
[0062] Sample loading: 2.0g (100ml).
[0063] Equilibrate the chromatographic column with 75%A+25%B for 5 minutes, load the sample, run gradient purification, monitor and collect the target peak fractions in three sections: before the peak, at the top of the peak, and after the peak. The pre-peak and post-pea...
Embodiment 3
[0075] Embodiment 3: the purification of ganirelix acetate
[0076] (1) Purification of ganirelix crude peptide:
[0077] Dissolve 2.0 g of crude ganirelix peptide in 100 ml of purified water and acetonitrile (v:v=3:1), filter, and collect the filtrate for later use.
[0078] Purification chromatography conditions:
[0079] Chromatographic column: 50×250mm, filled with octadecylsilane bonded silica gel as stationary phase filler.
[0080] Flow rate: 80ml / min.
[0081] Monitoring wavelength: 280nm.
[0082] Mobile phase A phase: 100mM sodium perchlorate solution, adjust the pH to 2.5 with phosphoric acid.
[0083] Mobile Phase B: Acetonitrile.
[0084] gradient:
[0085] Sample loading: 2.0g (100ml).
[0086] Equilibrate the chromatographic column with 75%A+25%B for 5 minutes, load the sample, run gradient purification, monitor and collect the target peak fractions in three sections: before the peak, at the top of the peak, and after the peak. The pre-peak and post-pe...
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