Establishment method of tissue-engineered human salivary gland pleomorphic adenoma living tissue model
A tissue engineering, living tissue technology, applied in the direction of tumor/cancer cells, vertebrate cells, animal cells, etc., can solve problems such as the inability to establish living tissue models
Inactive Publication Date: 2014-06-18
河北医科大学口腔医学院
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[0005] The purpose of the present invention is to provide a method for establishing a tissue-engineered human salivary gland pleomorphic adenoma living tissue model, to solve the problem that the current human salivary gland pleomorphic adenoma cannot establish a living tissue model, and to provide a method for benign human salivary gland pleomorphic adenoma that is difficult to study in vivo. Tumor research offers a living tissue model
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[0033] (1) Preparation of materials and reagents:
[0034] Preparation of culture medium Ⅰ: Take a small amount of RPMI-1640 culture medium, add 20ml fetal bovine serum, 1000U penicillin, 1000U streptomycin, mix well, add RPMI-1640 to make up to 100ml.
[0035] Preparation of culture medium II: Take a small amount of RPMI-1640 culture medium, add 10ml fetal bovine serum, 1000U penicillin, 1000U streptomycin, mix well, add RPMI-1640 to make up to 100ml.
[0036] Preparation of culture solution III: Take a small amount of RPMI-1640 culture solution, add 20ml fetal bovine serum, 1000U penicillin, 1000U streptomycin, 0.25g hyaluronic acid, mix well, add RPMI-1640 to make up to 100ml.
[0037] Preparation of treatment solution Ⅰ: Take a small amount of RPMI-1640 culture solution, add 10ml fetal bovine serum, 10000U penicillin, 5000U streptomycin, mix well, add RPMI-1640 to make up to 100ml.
[0038]Preparation of treatment solution II: Take a small amount of RPMI-1640 culture solu...
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The invention discloses an establishment method for a living tissue model of tissue-engineered human salivary gland pleomorphic adenoma. The method comprises the following steps of performing in-vitro co-culture on a scaffold tissue in the same medium after primary culture and subculture of a salivary gland pleomorphic adenoma tumor tissue and normal subcutaneous fibrous tissue cells which are taken from the same patient, and then transplanting into a dorsal subcutaneous tissue of nude mice to establish the living tissue model of the tumor. The living tissue model of tissue-engineered human salivary gland pleomorphic adenoma is established by using a tissue engineering method and technology. The establishment method provides research basis for studying pathogenesis of human salivary gland pleomorphic adenoma and investigating various innovative treatment methods, and simultaneously provides a method and an idea that can be used as reference for other living tissue models of benign tumors.
Description
technical field [0001] The invention relates to a method for establishing a tumor model, in particular to a method for establishing a tissue engineered human salivary gland pleomorphic adenoma living tissue model. Background technique [0002] Some benign tumors that are common in clinical practice are prone to recurrence or multiple occurrences. Inadvertent operation during surgery often causes postoperative tumors to grow in a beaded shape along the surgical incision. The implanted growth and recurrence of postoperative tumors bring great trauma and pain to patients physically and psychologically; multiple recurrences may also lead to malignant transformation of tumors, especially some benign tumors that occur in the oral and maxillofacial region, such as human Salivary gland pleomorphic adenoma is defined by the WHO as a borderline tumor. Due to the particularity of its location, it also brings difficulties to reoperation and seriously affects the quality of life of patie...
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IPC IPC(8): C12N5/09
Inventor 任贵云王洁董福生张艳宁
Owner 河北医科大学口腔医学院