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Bactrian camel alpha-lactalbumin gene, recombinant protein and cloning method thereof

A lactalbumin and recombinant protein technology, applied in the field of genetic engineering, can solve the problem of lack of Bactrian camel α-lactalbumin gene research and other problems, and achieve the effect of high application value

Inactive Publication Date: 2013-04-03
XINJIANG WANGYUAN CAMEL MILK IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many researches on casein and α-lactoglobulin at home and abroad, but there is almost no related research on the research on Bactrian camel α-lactalbumin gene in China

Method used

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  • Bactrian camel alpha-lactalbumin gene, recombinant protein and cloning method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: A Bactrian camel α-lactalbumin gene, which is a gene closely related to the functions of sterilization and induction of tumor cell apoptosis in addition to the characteristics of easy digestion and absorption in dairy products, with SEQ ID The nucleotide sequence from nucleotide 4-432 in NO.1.

Embodiment 2

[0026] Example 2: A recombinant protein of Bactrian camel α-lactalbumin gene.

Embodiment 3

[0027] Example 3: The nucleotide sequence of the recombinant protein is SEQ ID NO.1.

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Abstract

The invention relates to the technical field of genetic engineering and discloses a bactrian camel alpha-lactalbumin gene, a recombinant protein and a cloning method thereof. The gene has the characteristics of easy digestion and absorption, has a gene which is closely related to the functions of sterilizing and inducing apoptosis of tumor cells and has a nucleotide sequence from 4-432nd locus of the nucleotide in SEQ ID NO.1. The bactrian camel alpha-lactalbumin is one of main components of whey protein, has the function of regulating galactopoiesis, has the cytolysis activity, has the multiple functions of inducing cell growth inhibition and cell apoptosis and is an important protein in camel's milk. The established bactrian camel alpha-lactalbumin sequence and an evolutionary tree thereof can intuitively prove the difference between the bactrian camel alpha-lactalbumin and the protein sequence of other species, and experimental data are provided for addition of the camel's milk in food and further biochemical research; and therefore, the bactrian camel alpha-lactalbumin gene has high application value.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, and relates to a Bactrian camel α-lactalbumin gene, a recombinant protein and a cloning method thereof. Background technique [0002] α-lactalbumin (alpha-lactalbumin) is one of the main whey protein components, which has the function of regulating milk production, as well as cell lytic activity, inducing cell growth inhibition and apoptosis and other functions. Recent studies have found that it may have an anti-cancer function. In addition, lactalbumin is very similar to human milk in terms of amino acid ratio structure and functional properties. Clinical studies have shown that infant formula enriched with alpha-lactalbumin is safe. α-lactalbumin is rich in tryptophan, which helps to promote the baby's neurodevelopment and is an important factor in regulating sleep, appetite and mood. What's more, alpha-lactalbumin is an excellent source of essential and branched-chain amino acids...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/47C12N15/10
Inventor 陈钢粮
Owner XINJIANG WANGYUAN CAMEL MILK IND
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