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Recombinant pseudotyped baculovirus Bac-G-prM/E for preventing West Nile virus as well as vaccine and application thereof

A pseudotyped baculovirus and West Nile virus technology, applied in the field of animal virology and animal genetic engineering, can solve the problems of unfavorable real-time monitoring, unknown pathogen characteristics, etc., and achieve high safety, good immune effect and good immunogenicity Effect

Inactive Publication Date: 2015-01-14
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This chimeric vaccine produces a good immune effect, but this recombinant virus may produce unknown pathogenic characteristics, which is not conducive to real-time monitoring

Method used

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  • Recombinant pseudotyped baculovirus Bac-G-prM/E for preventing West Nile virus as well as vaccine and application thereof
  • Recombinant pseudotyped baculovirus Bac-G-prM/E for preventing West Nile virus as well as vaccine and application thereof
  • Recombinant pseudotyped baculovirus Bac-G-prM/E for preventing West Nile virus as well as vaccine and application thereof

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Embodiment 1

[0073] 1. Construction of pcDNA3.1-prM / E transfer plasmid

[0074] The prM / E gene fragment of West Nile virus (WNV) selected in the present invention (the nucleotide sequence is shown in SEQ ID NO: 1, and the sequence length is 2058bp) was synthesized by Shanghai Sangon Bioengineering Co., Ltd. Double digestion with BamH I and EcoR I (see figure 1 After A), it was inserted into the plasmid pcDNA3.1(+) (purchased from Invitrogen, agent of Wuhan Life Technology Co., Ltd.) that had been digested with BamH I and EcoR I. The transfer plasmid pcDNA3.1-prM / E was obtained (see Figure iA). The constructed transfer plasmid pcDNA3.1-prM / E was digested and identified with BamH I and EcoR I, and the identification results confirmed that the constructed plasmid was correct (see figure 1 B).

[0075] 2. Construction of pFastBac-G-prM / E and pFastBac-prM / E recombinant transfer plasmids

[0076] The eukaryotic expression plasmid pCDNA3.1-prM / E containing the West Nile virus (WNV) prM / E gene...

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Abstract

The invention relates to the construction, vaccine preparation and application of a recombinant pseudotyped baculovirus Bac-G-prM / E for expressing the prM protein and E protein of a West Nile virus and belongs to the technical field of animal virology and animal genetic engineering. The invention discloses a recombinant pseudotyped baculovirus Bac-G-prM / E for expressing the prM protein and E protein of the West Nile virus, and the recombinant pseudotyped baculovirus with the preservation number of CCTCC (China Center for Type Culture Collection) NO:V201130 is preserved in the CCTCC. The invention also discloses a preparation method of the recombinant pseudotyped baculovirus and an application of the recombinant pseudotyped baculovirus in the preparation of a vaccine for preventing the West Nile virus. The invention provides a novel genetic engineering vaccine for preventing the West Nile virus.

Description

technical field [0001] The invention belongs to the technical fields of animal virology and animal genetic engineering. It specifically relates to the construction of a recombinant pseudotyped baculovirus strain expressing West Nile virus prM / E protein, a vaccine prepared by using the recombinant virus and its application in preventing West Nile virus. Background technique [0002] West Nile Fever (WNF for short) is an acute infectious disease common to humans and various animals caused by West Nile Virus (WNV) infection. In 1937, WNV was first isolated from the blood of a febrile Ugandan woman in the Two Nile region and named West Nile virus. The outbreak occurred in the United States in 1999, and spread rapidly to most parts of the United States in the following years, resulting in more than 20,000 infections and hundreds of deaths (Nash D.F et al., The outbreak of West Nile virus infection in the New York City area in 1999 .N.Engl.J.Med.2001, 344:1807-1814), once caused...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01A61K39/12A61K48/00A61P31/14C12R1/93
CPCY02A50/30
Inventor 曹胜波朱碧波刘学芹徐敏叶静杨晓红陈龙陈焕春钱平肖少波
Owner HUAZHONG AGRI UNIV