Method for detecting isoniazid drug resistance of mycobacterium tuberculosis by using pyrosequencing technology
A technology for Mycobacterium tuberculosis and pyrosequencing, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of inability to quickly obtain drug resistance, increase in detection time, and many times of reactions, etc. To achieve the effect of reducing the number of reactions, the amount of detection reagents, and shortening the detection time
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[0024] Since the mutation of the KatG gene is mainly concentrated at the 315 site, the pyrosequencing detection method is mainly performed on the 315 site of the isoniazid KatG drug-resistant gene in the present invention. A method for detecting the drug resistance of Mycobacterium tuberculosis to isoniazid provided by the present invention comprises first performing PCR amplification on the KatG gene, and then performing pyrosequencing on the PCR amplification product to determine whether the KatG gene has a mutation site; Among them, pyrosequencing adopts SNP mode, and the nucleotide loading sequence is GTCACAGTCTG.
[0025] Pyrosequencing technology
[0026] Pyrosequencing (Pyrosequencing) technology is a brand-new DNA sequence analysis technology, which can quickly and accurately determine a short target fragment. This technique does not require electrophoresis, and the DNA fragments do not require fluorescent labeling, and the operation is extremely simple.
[0027] P...
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