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Natural lemon disinfectant and preparation method thereof

Technology of a disinfectant, lemon, applied in the direction of disinfectant, botany equipment and method, biocide, etc.

Inactive Publication Date: 2013-04-24
TIANJIN JINXIU IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature report about extracting fresh lemon with water-glycerin and further fermenting it into an antiviral and sterilizing disinfectant.

Method used

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  • Natural lemon disinfectant and preparation method thereof
  • Natural lemon disinfectant and preparation method thereof
  • Natural lemon disinfectant and preparation method thereof

Examples

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Effect test

preparation example Construction

[0020] Preparation of Candida tropicalis-Streptococcus faecalis solution: According to the conventional process of microbial fermentation engineering, the Candida tropicalis species preserved on the inclined plane were first activated with potato medium, and then passed through the test tube (about 10mL)-triangle flask (about 100mL)-large The triangular flask (about 1000mL) was gradually expanded and cultivated to prepare the first-grade seed bacterial liquid of Candida tropicalis; similarly, the streptococcus faecalis was activated with beef extract peptone medium, and gradually expanded and cultivated through test tube-triangle flask-large triangular flask , prepared as a first-class seed liquid of Streptococcus faecalis.

[0021] The secondary and tertiary expansion cultures of Candida tropicalis and Streptococcus faecalis were carried out with the corn flour-soybean meal-potato-sucrose culture medium.

[0022] Secondary expansion culture conditions (30L fermenter): mix equ...

Embodiment 1

[0048] 1 kg of fresh lemon, washed, removed residues and stalks, fully mashed, soaked in 10 kg of 50% glycerin-water by mass, and inserted into 1 kg of Candida tropicalis-Streptococcus faecalis bacteria liquid (effective number of viable bacteria 2* 10 9 cfu.mL -1 , prepared above), fermented at 30°C for 24 hours; sampled and inspected under sterile conditions, extracted 10mL of fermentation broth, diluted 100 times, and measured the absorbance (OD) at a wavelength of 660nm. After the OD value reached 0.5-0.7, the fermentation process ended , heated to 80°C for 10 minutes to sterilize. Squeeze and filter with double-layer gauze, the filtrate is tested, the acidity pH value is 2.5-3.5, and the vitamin C content is 150-200mg.L -1 , after passing the test, fill it and store it at room temperature.

Embodiment 2

[0050] 10kg of fresh lemons, washed, removed residues, stalks, fully mashed or minced, soaked in 150kg of 50% glycerin-water by mass, and inserted into 15kg of Candida tropicalis-faecalis mixed bacteria liquid (effective live Bacteria count 2*10 9 cfu.mL -1 ), fermented at 330°C for 20 hours; sampled and inspected under sterile conditions, extracted 10mL of fermentation broth, diluted 100 times, and measured the absorbance value (OD) at a wavelength of 660nm. After the OD value reached 0.5-0.7, the fermentation process was ended and heated to 85°C Keep for 15min to sterilize. Squeeze and filter with double-layer gauze, the filtrate is tested, the acidity pH value is 2.5-3.5, and the vitamin C content reaches 150-200mg.L -1 After filling, store at room temperature.

[0051] The pH value of the above-mentioned acidity adopts the precision test paper method; the vitamin C content adopts the 2,6-dichloroindophenol titration method of GB / T6195-1986.

[0052] The characteristics...

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Abstract

The invention discloses a natural lemon disinfectant and a preparation method thereof. According to the invention, fresh lemon is extracted in water-glycerin, and is fermented by using candida tropicalis-streptococcus faecalis; the material is sterilized, and is subjected to extrusion filtering. An obtained filtrate has substantial sterilizing and virus-killing disinfecting effects. The controlling over acidity and vitamin C content is adopted as quality control indicators. When the disinfectant is qualified according to inspection, the disinfectant is filled and stored under room temperature.

Description

technical field [0001] The invention relates to a lemon disinfectant and a preparation method thereof, in particular to an antiviral and bactericidal disinfectant extracted from lemons and a preparation method thereof. Background technique [0002] It is known that the extract of citrus fruit (skin) contains a large number of functional substances, such as flavonoids, limonoids, vitamins, polysaccharides, pectin, essential oils and the like. It is reported that citrus fruit (skin) has the functions of sterilization, disinfection and anti-oxidation. However, there is no literature report about extracting fresh lemon with water-glycerin and further fermenting it into an antiviral and sterilizing disinfectant. Disinfectant prepared from lemon extract, does not contain poisonous and harmful substances. Disinfectants can be widely used in hospital wastewater treatment, sterilization, disinfection and virus removal in slaughterhouses. It can also be applied to the disinfection ...

Claims

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Application Information

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IPC IPC(8): A01N65/36A01P1/00
Inventor 张清敏唐景春钟金松刘冰
Owner TIANJIN JINXIU IND
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