Flight mass spectrum biochip for career potential assessment and its detection method

A technology of flight mass spectrometry and biochips, applied in the field of gene chips, can solve the problems that personal career potential cannot be effectively evaluated, life lacks advance career planning, etc.

Inactive Publication Date: 2013-04-24
上海华翼生物科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0003] In order to overcome the problems in the prior art that the individual's career potential cannot be effectively assessed and the lack of advanc

Method used

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  • Flight mass spectrum biochip for career potential assessment and its detection method
  • Flight mass spectrum biochip for career potential assessment and its detection method
  • Flight mass spectrum biochip for career potential assessment and its detection method

Examples

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Embodiment Construction

[0016] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental method that does not indicate specific condition in the following examples, usually according to conventional conditions, such as Sambrook et al., molecular cloning: the conditions described in the laboratory manual (New York: Cold Spring Harbor Laboratory Press, 1989), or according to the manufacturer suggested conditions.

[0017] Step 1 Extract DNA from the subject sample

[0018] Using Wizard Genomic DNA purification Kit (Promega) or Tissue (MN) extracts DNA from tissues, cell blood samples or oral mucosa, quantifies it with a spectrophotometer, and checks its quality by agarose gel electrophoresis. The concentration of qualified DNA was adjusted to 50ηg / l, and st...

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PUM

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Abstract

The invention discloses a flight mass spectrum biochip for career potential assessment. The biochip includes a sample analyte and a mass spectrum chip matrix that undergo cocrystallization. The sample analyte comprises a?target?gene, a PCR primer and a single base extension primer used for innate potential assessment. The invention also discloses a detection method making use of the chip. The method consists of: extracting the sample DNA of a detection subject; designing a PCR primer and a single base extension primer; conducting target gene PCR amplification and single base extension on the sample DNA to form a sample analyte;?and?subjecting the sample analyte and the mass spectrum chip?matrix?to cocrystallization, and detecting the SNP locus of the target gene. The gene chip provided in the invention can?get an in-depth understanding of the potential ability of a person and assess personal career potential?so as to combine personal development with organizational development to work out corresponding work, education and training action plans, thus making reasonable arrangement of the time, projects and measures for each step.

Description

technical field [0001] The invention relates to a gene chip, in particular to a flight mass spectrometry biochip for occupational potential assessment and a detection method thereof. Background technique [0002] A biochip capable of more objective self-assessment is lacking in the prior art. Only by knowing yourself correctly can you make the right choice for your career, choose a career path suitable for your own development, and make the best choice for your career goals. Finally, combine personal development with organizational development. On the basis of analyzing personal and internal and external environmental factors, determine a person's career development goals, and choose a career or position to achieve this career goal, and prepare corresponding work, education and training. The training action plan makes reasonable arrangements for the time, items and measures of each step. Contents of the invention [0003] In order to overcome the problems in the prior ar...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N27/62
Inventor 张成虎
Owner 上海华翼生物科技有限公司
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