A method for simultaneous detection of cancer-associated fibroblasts and expressed proteins thereof

Abstract

The present invention discloses a method for simultaneous detection of cancer-associated fibroblasts and expressed proteins thereof, belonging to the technical field of tissue immunofluorescence. According to the method, a first type of quantum dot is used for labeling the antibody of a cell marker of the cancer-associated fibroblasts, or labeling a secondary antibody capable of specific binding to the antibody of the cell marker of the cancer-associated fibroblasts, or labeling streptavidin, and at least a second type of quantum dot is used for labeling the antibody of an expressed protein of the cancer-associated fibroblasts, or labeling a secondary antibody capable of specific binding to the antibody of the expressed protein of the cancer-associated fibroblasts, or labelling streptavidin; wherein the emission wavelengths of the first type of quantum dot and the second type of quantum dot are different from each other, and the first type of quantum dot and the second type of quantum dot does not label the streptavidin simultaneously. The invention also discloses a kit for simultaneous detection of cancer-associated fibroblasts and expressed protein thereof. The method of the invention is simple, efficient, accurate, and simultaneous in detection, suitable for promotion to the simultaneous labeling and quantitative analysis of a variety of other cancer-associated markers.

Description

technical field The invention belongs to the technical field of tissue immunofluorescence, and in particular relates to a labeling method for simultaneously detecting tumor-associated fibroblasts (Cancer associated fibroblasts, CAFs) and proteins expressed therein. Background technique In the process of tumor occurrence and development, the microenvironment acts as a "soil" and is an important condition for promoting tumor progression. The tumor microenvironment is composed of multiple components, including fibroblasts, immune or inflammatory cells, adipocytes, and vascular endothelial cells in addition to tumor cells themselves. Among them, the number of fibroblasts is more. Under the influence of tumor cells, a series of changes have taken place in the microenvironment to make it more suitable for the survival of tumor cells. CAFs are fibroblasts activated by tumor cells, and high expression of α-smooth muscle actin (α-smooth muscle actin, α-SMA) is one of its important ...

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So far, quantum dots have not been used for the l

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