Method for catalytic synthesis of monoglyceride and diacylglycerol by lipase under ultrahigh pressure

A technology of lipase and monoglyceride, applied in the field of bioengineering, can solve the problems of low enzyme catalytic efficiency and low product quality, and achieve the effects of good enzyme stability, high product quality and mild conditions

Inactive Publication Date: 2013-05-01
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to: in order to improve the low quality of the current glyceride products and the low catalytic efficiency of the enzyme, the present invention proposes a method for synthesizing monoglyceride and diglyceride catalyzed by lipase under ultra-high pressure. The reaction conditions of the method are mild and the used The enzyme is protected and activated by pressure, the reaction rate is fast, the loss of enzyme activity is small, the product quality can be greatly improved, the production cost can be reduced, and it has the prospect of industrial application

Method used

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  • Method for catalytic synthesis of monoglyceride and diacylglycerol by lipase under ultrahigh pressure
  • Method for catalytic synthesis of monoglyceride and diacylglycerol by lipase under ultrahigh pressure

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Weigh 50 g of olive oil, add 150 mL of 4% polyethylene glycol solution, and mix with a high-speed homogenizer at 10,000 rpm to form a microemulsion. Measure 100mL of microemulsion as a substrate, add wheat germ lipase with 20% olive oil quality, mix well, put it in a 100mL reaction bottle, and seal it. Control the temperature of the high-pressure equipment to 50° C., the pressure to 250 MPa, and the reaction time to 2 hours. After the reaction, the reaction mixture is separated and purified by molecular distillation. The purification process is divided into two stages. In the first stage, the temperature is controlled at 110 °C and the vacuum degree is 80 Pa. Under this condition, most of the water is removed; in the second stage, the temperature is controlled The temperature was 180° C., and the degree of vacuum was 30 Pa to obtain Composition 1.

Embodiment 2

[0029] The substrate components and operation steps are the same as in Example 1, and the temperature of the control equipment is 50° C., and the pressure is normal pressure (0.1 MPa). The mixture after the reaction was subjected to molecular distillation to obtain composition 2.

Embodiment 3

[0031] Weigh 15g each of glycerin and silica gel, mix and stir evenly, and dry in an oven at 50°C to remove water. In a 100mL reaction bottle, add 20g soybean oil, 30g immobilized silica gel and 50mL tert-butanol, and add porcine pancreatic lipase with 2% soybean oil mass. The controlled temperature is 60°C, the pressure is 150MPa, and the reaction time is 2.5h. After the reaction, the mixture was purified by molecular distillation. The molecular distillation stage is divided into two stages. In the first stage, the temperature is controlled at 130°C and the vacuum degree is 50pa to remove most of the glycerin; in the second stage, the temperature is controlled at 180°C and the vacuum degree is 20pa to obtain composition 3.

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Abstract

The invention discloses a method for catalytic synthesis of monoglyceride and diacylglycerol by lipase under ultrahigh pressure and belongs to the technical field of bioengineering. The method comprises the following steps of 1, dissolving reaction substrates in a solvent having a certain volume, adding a certain amount of lipase into the solution, and uniformly mixing, 2, putting the mixture into a ultrahigh pressure device, and carrying out an enzymic catalytic reaction process to obtain a mixture of fatty acid and glyceride, and 3, carrying out separation and purification of the mixture obtained by the step 2 by a molecular distillation technology to obtain monoglyceride and diacylglycerol. The method has the advantages of mild reaction conditions, high conversion rate, less enzyme activity loss, short reaction time, low production cost and large-scale application feasibility.

Description

technical field [0001] The invention relates to a method for synthesizing monoglycerides and diglycerides by lipase under ultrahigh pressure, belonging to the technical field of bioengineering. Background technique [0002] Monoglycerides and diglycerides are a class of polyol-type nonionic surfactants. Monoglycerides, diglycerides and their compounds are often used as emulsifiers, stabilizers, foaming agents, lubricants, anti-sticking agents, plasticizers and antibacterial agents, etc., and are widely used in daily chemicals, food, Pharmaceutical, textile printing and dyeing, metal processing and other industries. [0003] At present, the industrial synthesis of monoglycerides and diglycerides mainly adopts the glycerol hydrolysis method of oils, generally at high temperature (220-250°C) with inorganic bases as catalysts, under the protection of nitrogen to catalyze the reaction of oils and glycerin, and quickly after the reaction Lower the temperature to prevent the reve...

Claims

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Application Information

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IPC IPC(8): C12P7/64
Inventor夏小乐夏袖民温小荣张增英辛瑜王武
OwnerJIANGNAN UNIV