Method for detecting and quantifying utilization of carbon source in calcium carbonate by microalgae

A technology of calcium carbonate and microalgae, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of reducing the effectiveness of climate change policies and measures, affecting the accuracy of carbon sink estimation, ignoring utilization, etc. Achieve the effect of low cost, reliable data and simple calculation

Inactive Publication Date: 2013-05-01
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] The current estimation of ocean carbon sinks uses the flux of carbon dioxide in the atmosphere, ignoring the use of calcium carbonate, a huge carbon pool, by aquatic organisms in marine ecosystems, which seriously affects the accuracy of carbon sink estimation, leading to some Reduced effectiveness of changing policies and measures

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  • Method for detecting and quantifying utilization of carbon source in calcium carbonate by microalgae
  • Method for detecting and quantifying utilization of carbon source in calcium carbonate by microalgae
  • Method for detecting and quantifying utilization of carbon source in calcium carbonate by microalgae

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Embodiment Construction

[0047] Embodiments of the present invention: the first step is to measure the calcium carbonate stable carbon isotope composition δ produced by different manufacturers 13 C value and crystal form, choose two δ 13 Calcium carbonate powder with a C value difference greater than 8 ‰ and the same crystal form is added to the culture medium as isotope label 1 and isotope label 2 to cultivate the microalgae to be tested, and determine the stable carbon isotope composition of the initial microalgae to be tested δ 13 C value δ 0 and protein content N 0 . δ of isotope-labeled calcium carbonate 13 C value is recorded as δ Ci , where the δ of calcium carbonate labeled with 1 13 C value is δ C1 , the δ of isotope-labeled calcium carbonate 2 13 C value is δ C2 .

[0048] The second step is to measure the protein content of microalgae at different culture times under various culture conditions during the culture process. After the culture is over, the algae are harvested, and the ...

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Abstract

The invention discloses a method for detecting and quantifying utilization of carbon source in calcium carbonate by microalgae. The method comprises the following steps: adding two types of calcium carbonate powder with great difference in Epsilon 13 C value but same crystal form respectively to cultivate the to-be-detected microalgae, measuring the protein content of the microalage at different incubation time and Epsilon 13 C value of the stable carbon isotope composition of the microalage after a harvest; then, remedying the measured Epsilon 13 C value of the microalage at the time of the harvest by using the protein content of the initial microalage and Epsilon 13 C value, and obtaining the share of carbon source in the added calcium carbonate utilized by the microalgae by using the isotope mixed model; through the data about the protein content of the microalage at different incubation time and Redfield value, fitting an exponential growth equation about the biomass and time; finally, calculating the amount of carbon source in calcium carbonate obtained by the detected microalgae of biomass per unit in unit time according to the share of carbon source in the added calcium carbonate utilized by the microalgae and the equation about the biomass and time.

Description

technical field [0001] The invention relates to a method for detecting and quantifying calcium carbonate carbon source utilized by microalgae, which belongs to the fields of climate change response and marine bioengineering. Background technique [0002] Before the industrial revolution, the concentration of carbon dioxide in the air was only 280 ppmv, but now the concentration of carbon dioxide in the air has reached 391 ppmv, which has increased by 40%. Global change, dominated by the increase in the concentration of carbon dioxide in the atmosphere, brings not only ecological and economic problems, but also political problems to the world. Carbon content in global carbonate rocks is 5×10 21 mol, is considered the largest carbon pool. Calcium carbonate is the most important component of carbonate rock, and its proportion in carbonate rock is more than 50%. In addition, calcium carbonate deposits account for about 32.2% (average) of modern deep-sea sediments. Oceans and...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 吴沿友谢腾祥李海涛杭红涛刘丛强王宝利刘莹
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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