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A kind of preparation method of hyaluronic acid ester compound

A technology of hyaluronic acid ester and hyaluronic acid, applied in the biological field, can solve the problems of low reaction efficiency and long reaction time, and achieve the effects of simple preparation process, controllable degree of substitution of hydrophobic groups, and good research and development prospects.

Inactive Publication Date: 2015-10-21
OCEAN UNIV OF CHINA
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Problems solved by technology

However, since EDC is prone to produce more by-products in water solvents, the interference of water solvents also leads to low reaction efficiency and long reaction time.

Method used

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  • A kind of preparation method of hyaluronic acid ester compound
  • A kind of preparation method of hyaluronic acid ester compound

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Embodiment Construction

[0008] The present invention uses sodium hyaluronate and mono- and di-fatty acid glycerides as raw materials. In an anhydrous reaction system, the target product is synthesized by condensation esterification. First, the sodium hyaluronate is desalted and purified, and the carboxyl group is activated to form an intermediate product; The bond is covalently linked to the free hydroxyl groups of mono- and di-fatty acid glycerides to form a hyaluronic acid ester complex.

[0009] The specific implementation plan is:

[0010] 1. Desalination and purification of sodium hyaluronate:

[0011] Disperse sodium hyaluronate in 75% ethanol aqueous solution, the dosage of ethanol aqueous solution is 50-200ml / g sodium hyaluronate, fully swell under stirring, add concentrated hydrochloric acid dropwise, the molar ratio of hyaluronic acid and hydrochloric acid is 1:2, The reaction was continued for 30-60 minutes, and a precipitate was obtained after settling. It was washed with 75% alcohol sev...

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Abstract

The invention relates to a preparation method of hyaluronate compound, which comprises the following steps: desalting and purifying the raw material sodium hyaluronate; respectively dissolving the purified hyaluronate and mono / difatty glyceride in an anhydrous organic solvent, and protecting with nitrogen; adding a carboxyl activating agent into the hyaluronate solution, mixing with the mono / difatty glyceride solution, and continuing the reaction in the absence of air; and finally, centrifugally separating the supernatant, sufficiently dialyzing and carrying out freeze-drying. By utilizing the anhydrous reaction system to regulate and control the catalyst consumption and reaction material proportion, the substitution degree of the product is 4-23%, but the reaction time is shortened by 12-24 hours, thereby obviously enhancing the reaction efficiency. The preparation technique is simple; and the product has the advantages of controllable substitution degree of hydrophobic groups, high safety, no toxic or side effect and high stability. The invention has favorable research and development prospects in the fields of biomedicine, medical dressings, tissue engineering, cosmetics and the like.

Description

Technical field: [0001] The invention belongs to the field of biotechnology and relates to a method for preparing a hyaluronic acid ester complex. Background technique: [0002] Hyaluronic acid (HA) is a linear anionic polymer. It is a high-level polysaccharide composed of disaccharide units D-glucuronic acid and N-acetylglucosamine. It is widely distributed in the extracellular matrix of animal and human connective tissue. The content is higher in the vitreous body of the eye, umbilical cord, skin, cartilage and synovial fluid. HA has the advantages of high viscoelasticity, plasticity, permeability, unique moisture retention, excellent biocompatibility and degradability, and specific combination with specific receptors on the cell surface. Drug release and tissue engineering materials and medical dressings are currently widely used in the fields of biomedicine, tissue engineering and advanced cosmetics. Due to the poor stability, easy degradation and lack of biomechanical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08A61K47/36
Inventor 孔明陈西广程晓杰
Owner OCEAN UNIV OF CHINA
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