Supercharge Your Innovation With Domain-Expert AI Agents!

Mytilaria laosensis tissue culturing method

A tissue culture and rice row technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of large differentiation of seedlings and difficulty in obtaining maximum yield for plantations, and achieve the effect of shortening the breeding period.

Inactive Publication Date: 2013-06-05
广州市花木兰绿化有限公司
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Milaopai plantation in China all adopts seedling cultivation for afforestation, and the seedling differentiation is large, and it is difficult for the plantation to obtain the maximum yield

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mytilaria laosensis tissue culturing method
  • Mytilaria laosensis tissue culturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The applicant collected explants from the Milaopai experimental forest of Tropical Forestry Research Institute, No. 682, Guangshan 1st Road, Guangzhou City, Guangdong Province in August 2011, and propagated through tissue culture. By July 2012, 2000 seedlings had been produced for tropical The Chief Expert Group of the Research Institute of Forestry Economic Forest Cultivation and Utilization Theory and Technology Research builds a clone demonstration forest. Specific steps are as follows:

[0054] ⑴ Collection of explants

[0055] On August 3, 2011, the top shoots of the excellent plants that sprouted in the same year were collected, 25-30cm long, containing 4-7 pairs of leaves. The leaves were cut off at the time of collection, put into fresh-keeping dense bags, and brought back to the laboratory for disinfection on the same day .

[0056] ⑵ Disinfection of explants

[0057] In the laboratory, the branches were cut into 4-5cm long stems with 1-2 axillary buds in t...

Embodiment 2

[0076] The applicant collected excellent single plant explants of Milaopai plantation in Xijiang Forest Farm, Guangdong Province in July 2012. A total of 10 excellent single plant sprouted explants were collected. By October 2012, 9 excellent clones were collected. Proliferating buds were obtained, and the success rate of clone tissue culture reached 90%. The success rate of tissue culture = the clonal coefficient of obtained proliferating buds / the number of inoculated clones × 100%. After 3 months of multiplication culture, 278 multiplication buds were obtained from 9 clones. Lay a solid foundation for mass production of seedlings in 2013. Specific steps are as follows:

[0077] ⑴ Collection of explants

[0078] On July 12, 2012, 10 excellent single plants were collected from the top shoots of the sprouts in the same year, 25-30cm long, containing 4-7 pairs of leaves. The foam crisper was brought back to the laboratory in time.

[0079] The 2nd step and the 3rd step are ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a mytilaria laosensis tissue culturing method. According to the mytilaria laosensis tissue culturing method, high-quality tree coppice shoots are selected for establishing a tissue culturing vegetative propagation system, a large number of high-quality nursery stocks are cultured in a short term, the purpose of providing the high-quality clone mytilaria laosensis nursery stocks in a large scale is achieved so as to meet market demands, and therefore breeding years of mytilaria laosensis are greatly reduced, and meanwhile, a theoretical basis and a technical basis are provided for further development of a genetic improvement through a genetic engineering method.

Description

technical field [0001] The invention belongs to the technical field of cultivation and transplantation of tropical precious tree species, and in particular relates to a method for cultivating Mi Lao Pai. Background technique [0002] Mi Lao Pai ( Mytilaria laosensis Lecomte), also known as shell vegetable fruit and triangular maple, belongs to the evergreen broad-leaved tree of Hamamelidaceae. The height of the tree is more than 30m, and the diameter at breast height is 100cm. Naturally distributed in my country's Guangdong, Guangxi and Yunnan and Vietnam, Laos and other places. After the 1980s, it was successfully introduced to Fujian, Jiangxi, Zhejiang and other places in my country. Milaopai is a rare native broad-leaved tree species in the southern subtropical region of my country. In 1974, Yunnan Province selected Milaopai as an excellent timber tree species with high economic value and promising development prospects in tropical Yunnan. With the increase of the plan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 裘珍飞曾炳山范春节李湘阳刘英尹光天
Owner 广州市花木兰绿化有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com