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Beta-glucosidase, as well as expression gene and application thereof

A technology of glucosidase and gene expression, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as long time, low product yield, and inability to meet production requirements

Active Publication Date: 2015-03-18
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the technical methods for producing gentiobiose mainly include the following two types: 1. Extraction from natural raw materials, but the extraction yield of this method is low and the price is expensive, which cannot meet industrial production
2. Enzymatic production of gentiobiose, but the current enzymatic production of gentiobiose has inconvenient factors such as low enzyme activity and high enzyme production costs
However, the enzyme disclosed in this document takes too long to produce oligosaccharides, and the product yield is too low to meet the production requirements.

Method used

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  • Beta-glucosidase, as well as expression gene and application thereof
  • Beta-glucosidase, as well as expression gene and application thereof
  • Beta-glucosidase, as well as expression gene and application thereof

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Embodiment Construction

[0006] Technical scheme of the present invention is as follows:

[0007] A β-glucosidase, the amino acid sequence of which is shown in SEQ ID NO.1.

[0008] An expression gene of β-glucosidase, the nucleotide sequence of which is shown in SEQ ID NO.2.

[0009] A recombinant expression vector obtained by inserting the nucleotide sequence shown in SEQ ID NO.1 into the expression vector.

[0010] Preferably according to the present invention, the expression vector is a pET-32A expression vector.

[0011] A recombinant cell is obtained by transforming the above-mentioned recombinant expression vector into the cell.

[0012] Preferably according to the present invention, the cell is Escherichia coli BL21(DE3).

[0013] A method utilizing β-glucosidase to produce oligosaccharides, the steps are as follows:

[0014] The gene fragment whose nucleotide sequence is shown as SEQ ID NO.2 is introduced into the pET-32A plasmid vector, and then transformed into Escherichia coli BL21(DE3...

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Abstract

Provided is a β-glucosidase and expression gene and usage thereof. The amino acid sequence of the β-glucosidase is represented by SEQ ID NO.1. The nucleotide sequence of the expression gene of the β-glucosidase is represented by SEQ ID NO.2. The β-glucosidase may be used for production of oligosaccharides.

Description

technical field [0001] The invention relates to a beta-glucosidase and its expression gene and application, in particular to a beta-glucosidase and its expression gene and a method for producing oligosaccharides by using the enzyme, belonging to the technical fields of biotechnology and biochemical industry. Background technique [0002] Oligosaccharides are oligosaccharides composed of 2-10 monosaccharides, which have a very wide range of applications. For example, it is widely used in food, health products, beverages, medicine, feed additives and other fields. The United States, Japan, Europe and other places have large-scale production. The development and application of oligosaccharides in my country started in the mid-1990s and has developed rapidly in recent years. Common oligosaccharides include maltooligosaccharide glucose (α-1, 4 glycosidic bonds), gentiobiose glucose (β-1, 6 glycosidic bonds), oligosaccharide xylose (β-1, 4 glycosidic bonds combination), etc. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N15/56C12N15/63C12N15/70C12N1/21C12P19/14C12R1/19
CPCC12P19/04C12N9/2445C12N9/42C12P19/14C12N9/2434
Inventor 方诩高天龙王明钰刘奎美
Owner SHANDONG UNIV