Method for improving internal quality of low-class tobacco through solid fermentation

A technology of solid-state fermentation and tobacco leaves, which is applied in the fields of tobacco, tobacco preparation, and tobacco treatment. It can solve the problems of destructive effects on tobacco leaf quality and the growth of harmful microorganisms, and achieve the effects of improving smoking quality, inhibiting growth, and shortening time

Active Publication Date: 2013-06-26
SHANGHAI TOBACCO GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under the conditions of high material moisture and suitable temperature, harmful microorganisms are easy to bree

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Synergistic enzymolysis and fermentation of low-grade tobacco leaves with compound enzyme solution, fried rice juice and Bacillus amylophagous

[0043] 1 Experimental method

[0044] 1.1 Preparation of compound enzyme solution

[0045] Prepare compound enzyme solution: cellulase (7900PGNU / mL) 4mL, amylase (480KNU-B / g) 6g, glucoamylase (265,000 U / g) 4g, water 100mL. The enzymes were all produced by Novozymes Biotechnology Co., Ltd.

[0046] 1.2 Preparation of Bacillus amylophagosus seed suspension

[0047] 1) Incline cultivation

[0048] Bacillus thermoamylovorans (Bacillus thermoamylovorans, 10143-Bacillus thermoamylovorans provided by China Center of Industrial Culture Collection) was streaked and inoculated on the slant of broth medium, cultured at 45°C for 24h, Store in refrigerator at 4°C.

[0049] 2) Preparation of Bacillus thermophagocytic seed bacteria suspension

[0050] Take the preserved slant of Bacillus amylophagosus, add sterile water to make...

Embodiment 2

[0067] Example 2: Synergistic enzymolysis and fermentation of low-grade tobacco leaves with compound enzyme solution, fried rice juice and Bacillus amylophagous and microwave sterilization 1 Experimental method

[0068] 1.1 Preparation of compound enzyme solution

[0069] Compound enzyme solution: 4 mL of cellulase (900 PGNU / mL), 6 g of amylase (480 KNU-B / g), 4 g of glucoamylase (65,000 U / g), and 100 mL of water. The enzymes were all produced by Novozymes Biotechnology Co., Ltd.

[0070] 1.2 Preparation of Bacillus amylophagosus seed suspension.

[0071] 1) Incline cultivation

[0072] Bacillus thermoamylovorans (Bacillus thermoamylovorans, 10143-Bacillus thermoamylovorans provided by China Center of Industrial Culture Collection) was streaked and inoculated on the slant of broth medium, cultured at 45°C for 24h, Store in refrigerator at 4°C.

[0073] 2) Preparation of Bacillus amylophagicus seed solution

[0074] Take the preserved slant of Bacillus amylophagosus, add st...

Embodiment 3

[0095] Example 3: Synergistic enzymolysis and fermentation of low-grade tobacco leaves with compound enzyme solution, jujube juice and Bacillus thermohumidus and microwave treatment

[0096] 1 Experimental method

[0097] 1.1 Preparation of compound enzyme solution

[0098] Cellulase (7900PGNU / mL) 4mL, amylase (480KNU-B / g) 6g, glucoamylase (265,000 U / g) 4g, water 100mL; the enzymes are all produced by Novozymes Biotechnology Co., Ltd.

[0099] 1.2 Preparation of Bacillus amylophagicus seeds

[0100] 1) Incline cultivation

[0101]Bacillus thermoamylovorans, 10143-Bacillus thermoamylovorans provided by China Center of Industrial Culture Collection (China Center of Industrial Culture Collection) was streaked and inoculated on the slant of broth medium, cultured at 45°C for 24h, 4 ℃ refrigerator.

[0102] 2) Preparation of Bacillus amylophagosus seed suspension

[0103] Take the preserved slant of Bacillus amylophagosus, add sterile water to make bacterial suspension. Take ...

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PUM

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Abstract

The invention relates to a method for improving the internal quality of low-class tobacco through solid fermentation. A mixture liquid composed of a composite enzyme liquid, a tobacco quantifying liquid and a microorganism seed bacterium suspension into the low-class tobacco, and the low-class tobacco is subjected to enzymolysis and microorganism fermentation under solid condition, so as to improve the internal quality of the low-class tobacco. After the low-class tobacco is subjected to the solid fermentation treatment of the composite enzyme liquid, the quantifying liquid and the microorganism, the main chemical components in the low-class tobacco trend to be appropriate in content and coordinate in ratio, and the smoking quality is improved; under appropriate solid fermentation condition, by utilizing the technique, the growth of harmful microorganism in the low-class tobacco is effectively suppressed, the harmful microorganism is killed, and the time in solid fermentation of the tobacco is greatly shortened.

Description

technical field [0001] The invention relates to a method for solid-state fermentation to improve the intrinsic quality of low-grade tobacco leaves, belonging to the technical field of tobacco processing. Background technique [0002] my country is a big tobacco country. In the process of tobacco leaf production, about 10% of the annual flue-cured tobacco output belongs to low-grade tobacco or low-grade tobacco. These leaves, due to their poor intrinsic quality and poor industrial availability, have contributed to a certain level of stockpiling. Therefore, how to improve the intrinsic quality of low-grade tobacco leaves has become a common technical problem faced by many cigarette industry enterprises. [0003] The smoking quality characteristics of low-grade tobacco leaves are usually characterized by poor aroma quality, insufficient aroma, heavy miscellaneous gas (some low-grade tobacco leaves have obvious "green miscellaneous gas" or "endemic miscellaneous gas"), and rela...

Claims

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Application Information

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IPC IPC(8): A24B15/20A24B3/04
Inventor 汤朝起许赣荣李士林于兴伟束茹欣
Owner SHANGHAI TOBACCO GRP CO LTD
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