Method for regulating and controlling festuca arundinacea lawn soil nematode community structure by adopting complexant
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A technology for soil nematodes and tall fescue grass, which is applied in the restoration of polluted soil and other directions
Inactive Publication Date: 2013-07-03
TIANJIN NORMAL UNIVERSITY
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However, at present, there are relatively few studies on the effect of adding complexing agents on soil ecological characteristics, especially the research on the method of using complexing agents to regulate the soil nematode community structure of tall fescue turf has not been reported in the literature
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Embodiment 1
[0075] (1) Pass the collected soil samples through a 2 mm sieve and mix them for use; use tall fescue for lawn plants, and use disodium edetate, aminotriacetic acid or diethyltriaminetetraacetic acid as a complexing agent for use;
[0076] (2) The test uses a plastic flower pot with a height of 25 cm and a diameter of 12 cm. After washing and drying the gauze, it is cut into a round shape with a suitable size to fix the bottom of the flower pot. Each container weighs 2 kg, and 4.5 g of tall fescue seeds are sown;
[0077] (3) Quantitative water supply every day during the experiment to meet the needs of plant growth. When the tall fescue grows to the 28th-35th day, add the concentration of 5-10mmol·kg -1 EDTA, NTA or DTPA;
[0078] (4) During the cultivation period, the temperature was 25°C, the relative humidity was 31%, and the light conditions were natural incident light. The whole experiment lasted 120 days. On the 60th day of the experiment, the aboveground part of tall ...
Embodiment 2
[0081] (1) Pass the collected soil samples through a 2 mm sieve and mix them for use; use tall fescue for lawn plants, and use disodium edetate, aminotriacetic acid or diethyltriaminetetraacetic acid as a complexing agent for use;
[0082] (2) The test uses a plastic flowerpot with a height of 25cm and a diameter of 12cm. After washing and drying the gauze, it is cut into a round shape with a suitable size to fix the bottom of the flowerpot. Each container weighs 2kg, and 4.5g of tall fescue seeds are sown;
[0083] (3) Quantitative water supply every day during the experiment to meet the needs of plant growth. When the tall fescue grows to the 28th-35th day, add the concentration of 5-10mmol·kg -1 EDTA, NTA or DTPA;
[0084] (4) During the cultivation period, the temperature was 34°C, the relative humidity was 48%, and the light conditions were natural incident light. The whole experiment lasted 120 days. On the 60th day of the experiment, the aboveground part of tall fescue w...
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Abstract
The invention discloses a method for regulating and controlling a festuca arundinacea lawn soil nematode community structure by adopting a complexant. The method comprises the following steps: adopting a plastic flowerpot with a height of 25 cm and a diameter of 12 cm, shearing a gauze into a circle with appropriate size to fix the bottom part of the flowerpot after cleaning and drying, sowing 4.5 g festuca arundinacea seeds in each container which weighs 2 kg, supplying water quantitatively everyday during the experiment to satisfy the need of the plant growth, adding EDTA ( Ethylene Diamine Tetraacetic Acid ),NTA ( Nitrilo Triacetic Acid ) or DTPA ( Diethylene Triamine Pentaacetic Acid ) with a concentration of 5 to 10 mmol / kg<-1>respectively, and culturing under the situations that the temperature is 25-34 DEG C, the relative humidity is 31-48%, the light condition is natural incident light, and the whole test lasts 120 days. The experiment result shows that the adoption of complexant increases the quantity of the soil nematodes on the whole, and particularly increases the quantity of the plant parasitic type nematodes; meanwhile the quantity of the bacteria eating type and polyphagia feeding type nematodes is reduced, thereby influencing the health of the soil ecosystem.
Description
technical field [0001] The invention belongs to the technical field of environmental protection and relates to urban greening, in particular to a method for regulating the soil nematode community structure of tall fescue turf by using a complexing agent. Background technique [0002] Phytoremediation is a kind of contaminated soil remediation technology that has emerged in recent years. Due to its advantages of environmental friendliness, economy, in-situ remediation, and application to large areas, it has received extensive attention and has become a research hotspot in the field of environmental science. At present, in the research on the effect of adding complexing agents on plants at home and abroad, there are many applications of adding complexing agents to phytoremediation of heavy metal polluted soils. The low bioavailability of heavy metals is a very important limiting factor in the process of plant uptake of heavy metal ions, especially in neutral or alkaline soils....
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