Screening and identification kit of denitrifying bacteria and screening and identification method

A denitrifying bacteria and denitrification technology, applied in biochemical equipment and methods, microorganism-based methods, biochemical instruments, etc., can solve problems such as low efficiency and large workload, and achieve simple operation, small workload, screening and identification. Efficient effect

Inactive Publication Date: 2013-07-10
TIANJIN SPRING ENVIRONMENTAL TECH & ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, various methods for screening denitrifying bacteria have achieved good results, but the screening and identification of denitrifying bacteria are generally verified indirectly by using artificial culture medium, and the verification is not carried out in the sewage environment, or the results are simulated with the sewage environment. combined comparison
Moreover, the traditional verification method has the problems of heavy workload and low efficiency

Method used

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  • Screening and identification kit of denitrifying bacteria and screening and identification method
  • Screening and identification kit of denitrifying bacteria and screening and identification method
  • Screening and identification kit of denitrifying bacteria and screening and identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: A prefabricated tube for denitrification identification, including an identification tube main body 1, a gas collection tube 2, a support rod 6, a sealing cover 9 and an inoculation needle 10; the top of the identification tube main body 1 is conical, conical The top is provided with a top nozzle 13, and the top nozzle 13 is integrally connected to the gas collection pipe 2. The bottom of the support rod 6 is inserted into the gas collection pipe 2 from the top of the gas collection pipe 2, and the bottom of the support rod 6 is provided with a seal The external thread 4 is screwed with the sealing internal thread 3 provided at the bottom of the gas collection pipe. The bottom of the identification pipe main body 1 is provided with a bottom nozzle 8 with an external thread, and a nozzle sealing cover with an internal thread is screwed on the bottom nozzle 8 9. The end of the plastic inoculation needle 10 is provided with a connecting cap 12 with an internal...

Embodiment 2

[0043] Screening and identification of denitrifying bacteria:

[0044] The bacteria samples to be tested come from the strains isolated and purified in our laboratory from the sludge in the sewage aeration tank of Haihe Hospital, a total of 137 strains; and the sewage collected from Haihe Hospital in this example is used for verification to verify the denitrification ability of the bacteria samples to be tested. Compare with the identification results of the kit.

[0045] 1. Use the denitrification bacteria screening and identification kit to identify the denitrification function of the bacteria sample to be tested:

[0046] Step 1: Strain Enrichment

[0047] Inoculate the bacteria sample to be tested into the strain enrichment tube, tighten the cap of the strain enrichment tube, and place the strain enrichment tube flat on a shaker at 30°C and 150 rpm for 24 hours; the enrichment medium in the strain enrichment tube The ingredients are as follows:

[0048] The ingredients ...

Embodiment 3

[0066] Screening and identification of denitrifying bacteria:

[0067] The bacteria samples to be tested come from the strains isolated and purified from the aeration tank sludge of Tianjin Jizhuangzi Sewage Treatment Plant in our laboratory, a total of 96 strains; and the municipal sewage collected in this example is used to verify the denitrification ability of the bacteria samples to be tested. Verify and compare with the kit identification results.

[0068] 1. Use the denitrification identification kit to identify the denitrification function of the bacterial sample to be tested:

[0069] The method of using the denitrification screening and identification kit is the same as step 1 to step 4 in Example 2;

[0070] The composition of the enrichment medium in the strain enrichment tube in step 1 is as follows:

[0071] Each liter of enrichment medium contains: beef extract 1g, peptone 1g, glucose 2.1g, potassium dihydrogen phosphate 890mg, disodium hydrogen phosphate 1.5g,...

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Abstract

The invention provides a screening and identification kit of denitrifying bacteria and a screening and identification method. The kit comprises a denitrification identification prefabricated tube, a strain enrichment tube and a strain preservation solution. The screening and identification method comprises the following steps of: enriching strains to be detected by the strain enrichment tube and inoculating the strains into the denitrification identification prefabricated tube to be cultivated; meanwhile, storing an enriched bacteria solution by utilizing the strain preservation solution; and after the denitrification identification tube is statically cultivated, judging a strain denitrification capability according to color change and a gas production condition of a culture medium. The screening and identification kit and the screening and identification method disclosed by the invention have the advantages of realizing a portable and quantitative denitrifying effect on the denitrifying bacteria and realizing the one-station operation of cultivation, identification and preservation of the strains.

Description

Technical field: [0001] The invention belongs to a bacterial screening and identification method and a kit, in particular to a denitrifying bacteria screening and identification method and a kit for sewage treatment. Background technique: [0002] Ammonia nitrogen is one of the important substances that cause water pollution. At present, urban sewage contains a large amount of ammonia nitrogen. If it is directly discharged into the natural environment, it will cause serious environmental pollution, lead to eutrophication of rivers and lakes, and weaken the self-purification ability of water bodies. Biological denitrification is currently one of the most economical and effective methods for denitrification of wastewater, and biological denitrification of sewage includes nitrification and denitrification stages. The nitrification stage includes two stages: the NH 4+ -N oxidation to NO 2- -N ammonia oxidizing bacteria; the NO 2- -N oxidation to NO 3- -N nitrite oxidizing b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/04C12R1/01
Inventor 李博智武震宋文军魏纪平朱高雄
Owner TIANJIN SPRING ENVIRONMENTAL TECH & ENG
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