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Energy saving brewing method

A potential and functional technology, applied in beer brewing, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems such as no barley

Active Publication Date: 2013-07-17
CARLSBERG BREWERIES AS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this case, however, mutagenesis has not been practiced or successfully applied to barley

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0427] Screening for low LOX-2 activity in germinated barley germ

[0428] Improved screening material . Kernels collected from barley plants of the null-LOX-1 line Ca211901 produced by crossing (null-LOX-1 mutant D112×Jersey)×Sebastian were treated with the mutagen NaN according to the details provided by Kleinhofs et al. (1978). 3 Incubation. The barley null-LOX-1 mutant D112 is described in WO2005 / 087934 and deposited with the American Type Culture Collection (ATCC) on September 11, 2003 under the accession number PTA-5487 at the American Type Culture Collection (VA20110) MA University Street, Nassas City (10801).

[0429] This method was chosen because it is known to induce point mutations in barley genomic DNA, ultimately resulting in substitution or truncation of amino acid residues in the protein encoded by the mutagenized DNA. In the mutagenesis experiment of the present application, the mutant grains of the M1 generation were selected to experience subsequent two...

Embodiment 2

[0438] Screening for null MMT barley mutants

[0439] According to the detailed experiments provided by Kleinhofs et al. (1978), kernels collected from barley plants of cv. Prestige and cv. 3 Incubation. This method was chosen due to its known potential to induce point mutations in barley genomic DNA.

[0440] In this experiment, the mutant grains of the M1 generation were propagated in the field for the next two generations, and finally a high proportion of homozygous plants of the M3 generation were produced for screening purposes. Mutated kernels of generation M3 are expected to contain a frequency of 0.9-2.3 gene mutations per 10,000 kernels (Kleinhofs et al., supra). It should be noted that the M2 generation grain is not screened.

[0441] Interestingly, the present invention describes a rapid high-throughput screening method for the detection of M3 generation mutant barley kernels that lack MMT activity (lacking detectable SMM synthesis during malting). Therefore, th...

Embodiment 3

[0450] barley hybrid

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PUM

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Abstract

Barley based beverages are produced in large quantities, employing highly energy consuming methods, for example in the malting and brewhouse facilities for kiln drying and wort boiling operations, respectively. The present invention relates to energy saving methods for preparing barley based beverages, as well as to barley plants useful in such methods. In particular, the invention describes barley plants with combined traits of null-lipoxygenase-1 (null-LOX-1), null-lipoxygenase-2 (null-LOX-2) and null-S-adenosylmethionine:methionine S-methyltransferase in one plant, which is particularly useful for energy saving methods to prepare barley based beverages, such as beer.

Description

[0001] All patent and non-patent documents cited in this application are hereby incorporated by reference in their entirety. technical field [0002] The present invention relates to an energy-efficient method for preparing barley-based beverages, such as malt-based beverages, such as beer. The invention also relates to barley plants for use in the disclosed methods. In particular, the present invention describes a plant having null lipoxygenase-1 (null-LOX-1), null lipoxygenase-2 (null-LOX-2) and null S-adenosylmethionine:methylthio Barley plants characterized by the combination of amino acid S-methyltransferase (also called null-S-methionine (Met)-S-methyltransferase or null-MMT), i.e. null-LOX-1-null-LOX-2 - Null-MMT (herein also interchangeably referred to as double-null-LOX-null-MMT) barley plants for use in particular in the energy-efficient process for preparing barley-based beverages described herein. Background technique [0003] Malt mills and distilleries [00...

Claims

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Application Information

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IPC IPC(8): A01H5/10C12C1/18C12C7/00C12C12/00C12N9/10C12N15/82A23L7/25A23L7/20
CPCC12N9/1007C12C12/00A23L1/105A23L1/186C12N9/0069C12N15/8242C12C1/18C12Y201/01009C12Y113/11012C12N15/8243C12C7/00A23L1/185A23L2/38A01H5/10A23L7/20A23L7/25
Inventor 索伦·克努森普雷本·里斯伯吉特·斯卡德豪奇莱恩·M·贝克奥利·奥尔森
Owner CARLSBERG BREWERIES AS