Method for inducing masculinization of fugu rubripes at low temperature

A technology of redfin puffer and low temperature induction, applied in climate change adaptation, fish farming, application, etc., to achieve the effect of easy operation, conducive to large-scale production and use, and avoid operating procedures

Active Publication Date: 2015-07-01
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no report on the masculinization of redfin puffers induced by low temperature

Method used

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  • Method for inducing masculinization of fugu rubripes at low temperature
  • Method for inducing masculinization of fugu rubripes at low temperature
  • Method for inducing masculinization of fugu rubripes at low temperature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Select healthy male and female pufferfish with high plumpness and well-developed gonads for artificial induced insemination. The fertilized eggs are hatched for 50 days at a normal water temperature of 20°C, and then placed in a water temperature of 16°C for 50 days. Other conditions for hatching and cultivation with existing technology.

[0014] At the same time, fertilized eggs were hatched and cultivated for 100 days with normal water temperature (20° C.) according to the method of the prior art as a control group.

[0015] Gender identification: get 33 tails from the control group, and get 40 tails of the cultivated seedlings from Example 1 of the present invention, extract the gonads, fix with Bouin's solution, and change 80% alcohol after 6 hours. Dehydrated with conventional alcohol gradients, transparent in xylene, embedded in paraffin, and sliced ​​serially with a LEICA-RM 2016 microtome with a thickness of 7 μm. Stained with hematoxylin-eosin (H.E), mounted w...

Embodiment 2

[0020] Healthy male and female pufferfish with high plumpness and well-developed gonads were selected for artificial induced insemination. The fertilized eggs were hatched at 20°C for 75 days, and then placed in 15°C for 35 days.

[0021] The ratio of males in Example 2 of the present invention can reach 80%.

Embodiment 3

[0023] Healthy male and female pufferfish with high plumpness and well-developed gonads were selected for artificial induced insemination. The fertilized eggs were hatched at 20°C for 45 days, and then cultured at 17°C for 65 days.

[0024] The ratio of males in Example 3 of the present invention can reach 65%.

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Abstract

The invention discloses a method for inducing masculinization of fugu rubripes at a low temperature. The method comprises the following steps of: performing artificial induced spawning and insemination on healthy male and female fishes which are high in fatness and undergo good gonad development; hatching fertilized eggs under the water temperature condition of 20 DEG C for 45-75 days; and putting into water of 15-17 DEG C, and culturing for 35-65 days. Due to the adoption of the method, male fugu rubripes fries can be produced rapidly, the male rate in the fries is 65-80 percent, the average male rate is 75 percent, ultraviolet ray irradiation is not required, sex hormone is not fed, and complex operation processes are avoided; and the method is low in cost, is easy to operate, and has contribution to mass production.

Description

technical field [0001] The invention relates to a method for raising seedlings of red-finned puffer, in particular to a method for inducing masculinization of red-finned puffer at low temperature without ultraviolet radiation, without feeding sex hormones and with simple operation. Background technique [0002] Redfin Pufferfish ( Takifugu rubripes ) is the rare fish with the largest individual, the least toxicity and the highest economic value in the genus Puffer. Because of its delicious meat, rich in protein, low in fat and rich in vitamins B1, B2, selenium, zinc and other trace elements, it has always been favored by Japan, South Korea and Chinese, especially the Japanese will be red fin Oriental Pufferfish is regarded as the "king of fish", and pufferfish cuisine - "sashimi" is the leading dish. In addition, the Japanese transliteration name "Fugu" has auspicious meaning, so it has always been an important representative of Japanese food culture. [0003] Studies have...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李雅娟姜志强杨凤李佳奇王玉生
Owner DALIAN OCEAN UNIV
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