Microorganism concentration process and device
A concentration and polymer technology, applied in the field of capturing or concentrating microorganisms, can solve the problems of slow speed and high cost, and achieve the effect of low cost, simple cost, and promotion of sample processing.
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[0152] Objects and advantages of this invention are further illustrated by the following examples, but the particular materials and amounts thereof recited in these examples, as well as other conditions and details, should not be construed to unduly limit this invention. All parts, percentages, ratios, etc. in the following examples are by weight unless otherwise indicated. Solvents and other reagents were obtained from Sigma-Aldrich Chemical Company, Milwaukee, WI unless otherwise indicated. All microbial cultures were purchased from the American Type Culture Collection (ATCC; Manassas, VA) in Manassas, VA. Unless otherwise indicated, experimental results are the average of 2 tests. Overnight cultures were prepared by streaking selected microorganisms onto trypticase soy agar plates followed by overnight incubation at 37°C. All microbial counts were performed according to standard microbiological enumeration methods for colony forming units and counts are approximate.
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example 1-2 and comparative example C1
[0184] Example 1-2 and Comparative Example C1: Preparation of concentration devices 1, 2 and C1
[0185] By first 30g of 1 denier fibrillated polyethylene fiber (FYBREL TM 620 fiber; Minifibers, Inc., Johnson City, TN, Jackson City, Tennessee) with 4 L of cold tap water in a 4 L blender (Waring Commercial Heavy Duty Blender 37BL84 (Waring Commercial Heavy Duty Blender, Model 37BL84)) to prepare the fiber premix by blending at medium speed for 30 seconds. The fibers were at this point dispersed uniformly in water without knots or clumps, and 6 g of 0.25 inch long 6 denier chopped nylon fibers (Minifibers, Inc. , Johnson City, TN)) and 6 g of long glass fibers (microstrand 106-475 glass fibers; Schuller, Inc., Denver, CO)) were added to the fiber dispersion and mixed at low speed. Blend for 30 seconds.
[0186] The matrix composition was prepared by adding 1000 mL of the resulting fiber premix to a 4 L stainless steel beaker and using an impeller mixer (Fisher Scientific, S...
example 3-4 and comparative example C2
[0189] Examples 3-4 and Comparative Example C2: Tests on Concentrators 1, 2 and C1
[0190] A colony of Listeria innocua (ATCC 33090) from an overnight streak culture was inoculated in 5 mL of BHI broth and incubated at 30°C for 18-20 hours. will contain 10 8 The resulting culture of CFU / mL was diluted in buffer solution and inoculated to 100 mL of BHI broth to provide a solution containing 10 5 CFU / mL (total 2×10 7 CFU) of the bacterial suspension. Disc pieces (48 mm in diameter) were cut from the sheets of porous fibrous nonwoven matrix of Examples 1, 2 and C1 and sterilized at 121° C. for 15 minutes. The disk from Example 1 was inserted into the vacuum filtration device described above, and 100 mL of the bacterial suspension was poured through the disk in the device until the entire sample passed through the disk. The process was repeated using the disk from Example 2. Use totals containing 1×10 7 Bacterial suspension test for CFU of discs from Comparative Example C...
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