Microorganism concentration process and device
A microorganism and concentration technology, applied in the methods of supporting/immobilizing microorganisms, biochemical equipment and methods, and the determination/inspection of microorganisms, etc., can solve the problems of high cost, slow diagnostic application speed, etc. low cost effect
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[0089] Objects and advantages of this invention are further illustrated by the following examples, but the particular materials and amounts thereof recited in these examples, as well as other conditions and details, should not be construed to unduly limit this invention. All microbial cultures were purchased from the American Type Culture Collection (ATCC; Manassas, VA).
[0090] concentration agent
[0091] Crystalline magnesium silicate concentration agent (hereinafter, talc) was purchased from Mallinckrodt Baker Company (Phillipsburg, NJ).
[0092] Amorphous spheroidized magnesium silicate concentration agent (hereinafter, AS-talc) in 3M TM Cosmetic Microspheres CM-111 (formed into solid spheres; particle density 2.3 g / cm3; surface area 3.3 m 2 per gram; particle size: 90% less than about 11 microns, 50% less than about 5 microns, 10% less than about 2 microns; available from 3M Company, St. Paul, MN).
[0093] Zeta potential measurement
[0094] Using Colloidal Dy...
example 1
[0164] Bacterial colonies of isolated Salmonella enterica subspecies Salmonella typhimurium (ATCC 35987) were inoculated in 5 mL of BBL TM Trypticase TM soybean broth (Becton Dickinson, Sparks, MD) and incubated at 37°C for 18-20 hours. Bring the concentration to about 1 x 10 9 CFU / mL of this overnight culture was diluted in Butterfield's buffer (pH 7.2 ± 0.2; potassium dihydrogen phosphate buffer solution; VWR catalog number 83008-093, VWR, West Chester, PA) to obtain approximately 1 ×10 3 CFU / mL of inoculum.
[0165] Use about 1×10 3 A 1:100 dilution of the CFU / mL inoculum was inoculated into a 250 mL volume of drinking water (from a drinking fountain) to yield a sample concentration of about 11 CFU / mL (containing a total of about 2600 CFU in a sample of about 250 mL). Custom sample holders using concentrators (the holder consists of upper and lower flow distribution plates with machined plastic tubing to provide a friction fit for the 47mm diameter concentrator; O-rin...
example 2 and 3 and comparative example 2
[0177] Self-obtained blood agar incubated at 30°C for 18 hours was used in 3 mL of Butterfield buffer (pH 7.2 ± 0.2; potassium dihydrogen phosphate buffer solution; VWR Cat. No. 83008-093, VWR, West Chester, PA). 0.5 McFarland standard (using DensiCHEK TM Densitometer, bioMerieux Corporation, Durham, NC). will contain 1×10 8The resulting bacterial stock in CFU / mL was serially diluted in Butterfield's buffer to obtain approximately 1 x 10 3 CFU / mL of inoculum.
[0178] Bagged iceberg lettuce (Example 2) and organic spinach (Example 3) were purchased from a local grocery store (Cub Foods, St. Paul, MN). In sterile Stomacher TM 25 g each of lettuce and spinach (hereinafter, produce samples) were weighed into polyethylene filter bags (Seward, Norfolk, UK). will be approximately 1 x 10 3 A 1:1000 dilution of CFU / mL inoculum was inoculated on the produce samples to obtain a final concentration of 1 CFU / mL in each produce sample. Samples of each inoculated produce were mixed b...
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