Microorganism concentration process and device
A microorganism and concentration technology, applied in the field of device preparation, capture or concentration of microorganisms, can solve the problems of expensive, slow diagnosis and application, and achieve the effect of sample processing elimination, simple cost, and high resistance to blocking.
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example 1-2 and comparative example C-1-C-4
[0197] Food samples (pasteurized orange juice (without pulp), sliced ham luncheon meat, and sliced chicken luncheon meat) were purchased from a local grocery store (Cub Foods (St. Paul, MN)). Separately in sterile Stomacher TM 25 mL of orange juice and 25 grams of each sliced meat were weighed into polyethylene filter bags (PE-LD Model 400, Seward Corp (Norfolk, UK)). The resulting samples were each obtained from an overnight tryptic soybean broth culture (essentially as above based on the title "Concentration Agent Screening: Microorganism Concentration Test Method (Concentration Agent Screening: Microorganism Concentration Test Method)" Prepared as described, except using Bacto TM Salmonella enterica subspecies Salmonella typhimurium (ATCC 35987) and Escherichia coli bacteria (ATCC 51813) were inoculated at a concentration of about 1 CFU / mL from Tryptic Soy Broth (Becton Dickinson (Sparks, MD)).
[0198] After the inoculated samples were incubated at room temperatur...
example 3
[0205] Pasteurized apple juice was purchased from a local grocery store (Cub Foods (St. Paul, MN)). 25 mL of apple juice was mixed with 225 mL of sterile Butterfield's Buffer (pH 7.2, VWR (West Chester, PA)) and inoculated with Salmonella enterica subsp. typhimurium (ATCC 35987) at a concentration of approximately 100 CFU / mL. The resulting sample was incubated at room temperature (approximately 23° C.) for 10 minutes and then pumped (essentially as described above) through Form B (TiO 2 - DE) Concentration device (prepared essentially as above) and for 25 minutes. The flow-through sample fraction (1 mL) was collected in labeled sterile 5 mL polypropylene tubes (BD Falcon TM , Becton Dickinson (Franklin Lakes, NJ)) and were plated in 3M according to the manufacturer's instructions TM Petrifilm TM Aerobic Count Plates medium (dry, rehydratable; 3M Company (St. Paul, MN)).
[0206] After the sample has passed through the concentration device, the device is placed (using ste...
example 4
[0214] Inoculate 5 mL of BBL with isolated bacterial colonies of Salmonella enterica subspecies Salmonella typhimurium (ATCC 35987) TM Trypticase TM Soy Broth (BBL TM Trypticase TM Soy Broth) (Becton Dickinson (Sparks, MD)) and incubated at 37°C for 18-20 hours. Bring the concentration to about 1 x 10 9 CFU / mL of this overnight culture was diluted in Butterfield's Buffer (pH 7.2 ± 0.2; potassium dihydrogen phosphate buffer solution; VWR catalog number 83008-093, VWR (West Chester, PA)) to obtain approximately 1 × 10 3 CFU / mL of inoculum.
[0215] Use about 1×10 3 A 1:100 dilution of the CFU / mL inoculum was inoculated into a 250 mL volume of drinking water (from a drinking fountain) to yield a sample concentration of about 16 CFU / mL (containing a total of about 4000 CFU in a 250 mL sample). The sample was pumped (essentially as described above) through Form A (Fe 2 o 3 - DE) Concentration device (prepared essentially as above) and for 25 minutes. Flow-through sample...
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