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Fibrinogen compound and application thereof

A technology of fibrinogen and complexes, applied in the direction of peptide/protein components, active ingredients of hydroxyl compounds, drug combinations, etc., can solve problems such as no effect and ineffective visual function of the host

Inactive Publication Date: 2015-01-14
SHANGHAI SHENYIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the research can only do the survival and differentiation of transplanted cells
However, the integration of transplanted cells and host cells and the recovery of the host's visual function are not obvious, or even have no effect at all.

Method used

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  • Fibrinogen compound and application thereof
  • Fibrinogen compound and application thereof
  • Fibrinogen compound and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Cell Culture and Acquisition

[0027] 1.1 About retinal ganglion cells (Retinal stem cell, RGC)

[0028] Retinal ganglion cells are located in the innermost layer of the retina, the ganglion cell layer. The axons of RGCs gather into bundles and leave the eye to form the optic nerve, which forms the optic tract with the optic nerve from the other eye at the optic chiasm, and finally projects to the lateral geniculate body. RGCs are interconnected and form a network of interactions, and their axons are also closely associated with each other. Neuronal axons absorb neurotrophic factors and transport them to the cell body through retrograde transport, so axonal necrosis can cause neuronal cell body malnutrition or even death. A very small number of RGCs or their axons degenerate into necrosis or function, which will lead to progressive dysfunction of the remaining normal RGCs and their axons, and eventually decompensation to apoptosis of all cells leading to bli...

Embodiment 2

[0054] Preparation of embodiment 2 fibrinogen complex

[0055] Add NaCl to deionized water to make 10ml of 0.8% (w / v) NaCl solution, filter through the filter head, dissolve 400mg of fibrinogen in it, add the neurotrophic factors shown in Table 1 in turn, and finally add prothrombin , to obtain the nerve regeneration biological glue of the present invention, wherein the final concentration of fibrinogen is 22-28U / ml, and the final concentration of thrombin is 22-28U / ml. The bioglue is formed about 15 minutes after preparation, has high viscosity, high mechanical strength, and large porosity, and is easier to fix at the injection site, and is suitable for injection at the site of optic nerve injury in living bodies (animals / humans).

Embodiment 3

[0056] Embodiment 3 animal experiments

[0057] 3.1 Experimental animals

[0058] Healthy Sprague Dawley rats, male or female, weighing 250g-300g, examined under a slit-lamp microscope, had double gills as large and round as L, responded well to light, had no obvious eye disease, and no crooked neck. Rats were anesthetized by intraperitoneal injection of 3% pentobarbital sodium solution, 1ml / kg, and the middle part of the upper eyelid was cut perpendicular to the eyelid margin under the binocular operating microscope until the supraorbital edge, and the bulbar conjunctiva and part of the fornix conjunctiva were cut in the same direction , expose and separate the superior rectus muscle, bluntly dissect the fascia along the direction of the superior rectus muscle to expose the optic nerve, cut the superior rectus muscle, clamp the severed end of the superior rectus muscle insertion with micro-toothed forceps, pull the eyeball downward, and fully expose the optic nerve. A specia...

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Abstract

The invention discloses a fibrinogen compound and an application thereof. The fibrinogen compound comprises fibrinogen, prothrombin and eleven neurotrophic factors. The fibrinogen compound can be used for preparing drugs for treating glaucoma, hypophysoma, traumatic optic nerve injury, operation caused optic nerve injury and the like; and when the compound is combined with transplanted cells, the transplanting repair effect can be enhanced.

Description

technical field [0001] The invention belongs to the field of medical biomaterials, and relates to a fibrinogen compound and its application, in particular to a fibrinogen compound and its application in the preparation of drugs for inner retinal diseases and other diseases causing optic nerve damage . Background technique [0002] Optic nerve damage and glaucoma are two important causes of blindness in patients. Among them, there are multiple reasons that may lead to optic nerve damage, such as trauma, surgery involving the optic nerve, etc. The current clinical treatment methods for optic nerve injury are poor. Only a small number of patients have a slight improvement in vision, and most patients have no improvement in vision. Glaucoma is a type of eye disease caused by progressive increase in intraocular pressure, and it is the second leading cause of blindness in the world. Current clinical treatment methods can only delay the progression of the disease, but cannot imp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/55A61P27/02A61P25/00A61K31/203
Inventor 朱剑虹高华嵩朱侗明
Owner SHANGHAI SHENYIN BIOTECH
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