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Application of ATF (activating transcription factor) 3 to predicting bladder cancer metastasis and judging prognosis

An ATF3, bladder cancer technology, applied in the field of oncology and diagnosis, which can solve the problems of lack of protein markers, lack of markers to detect or judge the metastasis of bladder cancer, etc.

Inactive Publication Date: 2013-08-07
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently there is still a lack of satisfactory protein markers for cancer detection, and even less markers that can be used to detect or judge the metastasis of bladder cancer

Method used

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  • Application of ATF (activating transcription factor) 3 to predicting bladder cancer metastasis and judging prognosis
  • Application of ATF (activating transcription factor) 3 to predicting bladder cancer metastasis and judging prognosis
  • Application of ATF (activating transcription factor) 3 to predicting bladder cancer metastasis and judging prognosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Specimen: Freshly resected tissue samples from patients with clinical bladder cancer

[0035] 2. Antibody: Anti-ATF3 antibody (HPA001562), rabbit anti-human polyclonal antibody, purchased from Sigma Aldrich (USA).

[0036] 3. Immunohistochemical method:

[0037] Paraffin sections were prepared from freshly resected tissue samples from patients with clinical bladder cancer. The paraffin sections were dewaxed with xylene and graded alcohol to water, and washed with PBS. Add 50 μl 0.3% H to each slice 2 o 2 , Block endogenous peroxidase activity. The slices were placed in antigen retrieval solution (Tris-EDTA, pH9.0) and microwaved for 30 min. Add 50 μl of blocking serum after the slices are equilibrated to room temperature, absorb excess serum after 10 min, then add 50 μl of rabbit anti-human ATF3 antibody, or PBS instead of primary antibody to exclude false positives, add biotin-labeled anti-rabbit II after overnight at 4 oC Resistance, 10 min at room temperature...

Embodiment 2

[0047] A kit for detecting or judging metastasis of bladder cancer. The kit is used to detect the expression of ATF3 protein in surgically resected tissue sections of bladder cancer patients through immunohistochemical technology. The detection method refers to Example 1. The expression of the ATF3 protein is negatively correlated with the degree of invasion and metastasis of bladder cancer. That is, bladder cancer patients with low expression of ATF3 are more likely to metastasize.

Embodiment 3

[0049] A kit for bladder cancer prognosis judgment, the kit is used to detect the expression of ATF3 protein in surgically resected tissue sections of bladder cancer patients through immunohistochemical technology. The detection method refers to Example 1. The expression of the ATF3 protein is positively correlated with the prognosis and survival period of bladder cancer patients. That is, the survival time of bladder cancer patients with low ATF3 expression is relatively shorter than that of bladder cancer patients with high ATF3 expression.

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Abstract

The invention relates to application of an ATF (activating transcription factor) 3 protein to preparation of a kit for detecting or judging bladder cancer metastasis and judging bladder cancer prognosis. The invention also provides the kit for detecting or judging bladder cancer metastasis and judging bladder cancer prognosis. The invention has the following advantages: the invention provides a new protein marker for predicting bladder cancer metastasis of patients with bladder cancers and judging prognosis and the protein marker can be used for detecting or judging bladder cancer invasion and metastasis degrees and judging prognosis by an immunohistochemical detection method; the invention provides the new kit for predicting bladder cancer metastasis of the patients with bladder cancers and judging prognosis; and the invention provides the new application of the ATF 3 protein to suppressing bladder cancer metastasis and lengthening the prognostic lifetime of the patients with bladder cancers.

Description

technical field [0001] The invention relates to the field of oncology and diagnosis, specifically, the application of ATF3 in predicting bladder cancer metastasis and judging prognosis. Background technique [0002] Bladder cancer is a common malignant tumor of the human genitourinary system (Siegel et al., 2013). In China, the incidence of bladder cancer is on the rise, ranking first among male genitourinary system tumors. Invasion and metastasis are the main causes of treatment failure and death in patients with bladder cancer. However, the exact mechanism of bladder cancer metastasis has not yet been clarified, and protein markers for judging bladder cancer metastasis are lacking clinically. [0003] Activator of transcription 3 (ATF3) is a stress-response-induced gene, which is located on chromosome lq32.3 and encodes a DNA-binding protein of 181 amino acids (Liang et al., 1996). The ATF3 gene contains 4 exons A, B, C, and E, and is a transcription factor containing a ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 沈立松许大康袁向亮
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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