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Simple and rapid propagation method of EM

A technology of strains and original species, applied in the direction of fungi, bacteria, etc., can solve the problems of complicated and many process steps, and achieve the effect of high bacterial count, simple process and good effect

Active Publication Date: 2014-12-03
重庆大方生态环境治理股份有限公司
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention requires a specific fermenter in the process of implementation, and a wide variety of raw materials are used; the cultivation process is also divided into multiple stages, so that the process steps are many and complicated.

Method used

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Embodiment Construction

[0047] Below in conjunction with embodiment the method of the present invention is further described.

[0048] A kind of EM is easy, the method for rapid propagation, and concrete implementation method is:

[0049] 1. A method for simple and rapid propagation of EM. The technical proposal includes: optimal preparation of liquid medium; selection of culture containers; selection of strains; method and process of cultivation.

[0050] 2. A method for EM simple and rapid propagation, the ratio of various raw materials of the liquid medium in the technical scheme is as follows:

[0051] (1) Rice flour: 1 kg.

[0052] (2) Corn flour: 0.5 kg.

[0053] (3) Brown sugar: 2 kg.

[0054] (4) Yeast powder: 0.5 kg.

[0055] (5) Peptone: 100 grams.

[0056] (6) Potassium dihydrogen phosphate: 150 grams.

[0057] (7) Vitamin C (D-isoascorbic acid) 75 grams.

[0058] (8) Water: 50 kg.

[0059] 3. A method for EM simple and rapid propagation, the preparation method of the liquid medium...

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Abstract

The invention relates the microbial preparation field, in particular to a simple and rapid propagation method of EM (effective microorganisms). Specifically, the method includes: optimized preparation of a liquid medium, selection and application of a culture vessel, strain selection, and cultivation method and process of EM. The method provided in the invention is characterized in that: cheap and easily available rice meal, brown sugar and corn meal are taken as main nutrient sources to prepare the liquid medium with reasonable and comprehensive nutrition; by reasonably selecting a white plastic bucket or a glass bucket (bottle) as the culture vessel and then adjusting the temperature and illumination intensity of the cultivation environment, the EM product with high quality and strong vitality can be obtained by only 3-4 days. The EM product produced by the method provided in the invention has a high bacterium content and good quality, and can be widely applied in crop farming, breeding industry, food processing, environmental protection and other fields.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to an EM simple and rapid propagation method. technical background [0002] EM is the abbreviation of English (Effective Microorganisms), translated into Chinese as: "Effective Microorganisms". It is composed of more than 80 species of microorganisms in 5 families, 10 genera, including photosynthetic bacteria, lactic acid bacteria, yeast flora, actinomycetes, and filamentous flora. of. EM technology is currently the most widely used bioengineering technology in the world. Compared with general biological agents, it has the advantages of complex structure, stable performance and complete functions, showing an unprecedented high-tech level. According to relevant reports, EM is applicable to various fields such as planting, breeding, environmental protection, and human health care. [0003] In recent years, due to the use of chemical fertilizers and pesticides by humans, the be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16
Inventor 何寒
Owner 重庆大方生态环境治理股份有限公司
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