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Preparation method of antioxidant activity mixed peptide originated from chick embryo

An antioxidant activity, mixed peptide technology, applied in cosmetic preparations, skin care preparations, medical preparations containing active ingredients, etc., can solve the problem of extracting antioxidant peptides, etc., to achieve high antioxidant activity and high safety. , The effect of low processing cost

Inactive Publication Date: 2013-08-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant technical report on the extraction of antioxidant peptides from chicken embryos using a simple separation method.

Method used

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  • Preparation method of antioxidant activity mixed peptide originated from chick embryo
  • Preparation method of antioxidant activity mixed peptide originated from chick embryo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation method of chicken embryo antioxidant mixed peptide:

[0026] (1) Select fertilized eggs that are not harmful to health, and put them in the incubator for incubation. The incubation temperature is controlled at 37.8°C, the relative humidity is 60%, and the eggs are turned every 2 hours. After hatching until the 15th day, the embryo is in the growth of nutrients During the peak period, various organs are basically formed. At this time, the fertilized eggs are taken out, placed in a 4°C freezer to finish hatching and stored for processing;

[0027] (2) Disinfect the surface of the eggs, remove the eggshell and take out the chicken embryos. Homogenize the chicken embryos with a tissue homogenizer at 4°C, pour them into a tray and put them in a freeze dryer for freeze-drying. After the samples are completely frozen After drying, crush it into powder, seal it and store it in a -30°C refrigerator for processing;

[0028] (3) Add sterile water to the dried chicken ...

Embodiment 2

[0033] Preparation method of chicken embryo antioxidant mixed peptide:

[0034] (1) Select eggs hatched to the 15th day for processing;

[0035] (2) Disinfect the surface of the eggs, remove the eggshell and take out the chicken embryos. Homogenize the chicken embryos with a tissue homogenizer at 4°C, pour them into a tray and put them in a freeze dryer for freeze-drying. After the samples are completely frozen After drying, crush it into powder, seal it and store it in a -30°C refrigerator for processing;

[0036] (3) Add sterile water to the dried chicken embryo powder to reconstitute according to the ratio of 5g powder / 100mL sterile water, and stir evenly to obtain chicken embryo slurry;

[0037] (4) Centrifuge the chicken embryo slurry at 4°C and 5000×g for 20 minutes, and collect the supernatant after centrifugation;

[0038] (5) Collect the supernatant after centrifugation, perform ultrafiltration through an ultrafiltration membrane with a molecular weight cut-off of 3...

Embodiment 3

[0041] Activity Determination of Chicken Embryo Antioxidant Mixed Peptides

[0042] (1) Materials: Potassium ferricyanide, ferric chloride, DPPH, and pyrogallol were purchased from Sigma; reagents such as trichloroacetic acid (TFA) were of chromatographic grade.

[0043](2) Extraction of active peptides, see Example 1 or 2.

[0044] (3) Determination of antioxidant activity:

[0045] a. Reducing power:

[0046] Take 10mL (1mg / mL) of the above ultrafiltrate, mix with 2.5mL of 0.2M phosphate buffer (pH6.6) and 2.5mL of 1% potassium ferricyanide solution, react at 50°C for 20 min, and add Add 2.5mL of 10% trichloroacetic acid solution; after fully shaking, centrifuge the mixture at 2000×g for 10 min; then absorb 2.5mL of supernatant, 2.5mL of deionized water, 0.5mL of 0.1% trichloroacetic acid Mix the ferric chloride solution, react at room temperature for 10 minutes, and measure its absorbance value at 700nm; the greater the absorbance value, the stronger the reducing power, ...

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Abstract

The invention discloses a preparation method of antioxidant activity mixed peptide originated from chick embryos, belonging to the technical field of bioactive peptide extraction. The preparation method comprises the following steps of: collecting healthy chick embryos which are incubated for certain days, and extracting a mixed peptide finished product with the antioxidant activity from the chick embryos through machining methods of homogenating, freezing, drying, re-dissolving with sterile water, centrifuging at low temperature, filtering by using an ultra-filtration membrane, freezing, drying and the like, so as to obtain a mixed peptide finished product with antioxidant activity is extracted from the chick embryos. The finished product can be added into food or cosmetics as functional factors, or is developed as an ingredient of a novel, natural and efficient antioxidant medicine. The method is simple, the cost is low, the obtained product has the advantages of nature, health, high activity and the like, and particularly the product has potential development values for the middle aged or the young in sub-health; and meanwhile as the natural anti-oxidative peptide is applied to health-care food and cosmetics, the consumption requirements of people on origin and pure nature are met. The preparation method is also a high additional value machining method of the chick embryos, and is an application of the food-medicine homology theory in traditional Chinese medicine in the health-care field.

Description

technical field [0001] The invention relates to a method for extracting mixed peptides with antioxidant activity from chicken embryos, and belongs to the technical field of active peptide extraction. Background technique [0002] Since Harman proposed the theory of free radicals in 1956, people have gradually realized that free radicals produced by oxidation reactions in the human body are closely related to the aging of the human body and chronic diseases such as arthritis, diabetes, hypertension, and hyperlipidemia. In today's society, with the improvement of people's living standards, various chronic diseases caused by free radicals that threaten human health have attracted more and more attention. In order to prevent these chronic diseases and achieve the purpose of delaying aging, people need to take effective methods to remove excessive active oxygen and free radicals in the body. Antioxidants can prevent chronic diseases and delay aging by eliminating free radicals i...

Claims

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Application Information

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IPC IPC(8): A23J1/08A61K35/54A61K38/00A61K8/98A61K8/64A61P39/06A61Q19/08A61K35/57
Inventor 徐学明段翔杨哪吴凤凤金征宇周星田耀旗焦爱权王金鹏赵建伟
Owner JIANGNAN UNIV
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