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Needle mushroom strain detection culture medium and usage method thereof

A culture medium and Flammulina velutipes technology, applied in application, fertilizer mixture, fertilization device, etc., can solve the problems of no detection method for strain degradation, errors in identification results, and degradation of strain activity, and achieve easy access to medicines and high accuracy , The effect of short detection cycle

Inactive Publication Date: 2013-08-28
SHANXI JIAQIN AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the continuous passage of Flammulina velutipes strains in PDA medium, the activity of the strains will be degraded. If the low-activity strains are not found until the fruiting body is formed in this continuous production process, serious economic losses will be caused.
[0003] At present, there is no detection method for strain degradation in the factory production of Flammulina velutipes. Most factories rely on the experience of technicians to identify the strains, and there are large errors in the identification results. Usually, a large number of degenerated strains are used, resulting in large-scale production reduction of Flammulina velutipes or The problem was discovered when there were no mushrooms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Wash and peel the fresh potatoes and cut them into thin slices. Weigh 200 parts of potato slices and place them in a large beaker. Add 1000 parts of sterile water, boil for 20 minutes, filter and take the filtrate, and add 18 parts of lactose and NH to the filtrate. 4 NO 3 2.5 parts, KH 2 PO 4 1.5 parts, MgSO 4 ·7H 2 1.5 parts of O, 0.06 part of bromothymol blue, after mixing uniformly, the pH value is adjusted to 7.0 to obtain a culture solution, and the culture solution is sterilized at 121° C. for 30 min to obtain the detection medium of Flammulina velutipes strains;

[0014] After cooling, place the obtained culture medium in a petri dish, inoculate the mycelia to be tested into the detection medium of Flammulina velutipes strains in a clean bench, and shake and culture at 24°C and 120r / min for 8 days;

[0015] Observe the decolorization of the culture medium. Those that can decolorize the medium are active strains, and those that make the medium color lighter...

Embodiment 2

[0017] Wash and peel the fresh potatoes and cut them into thin slices. Weigh 100 parts of potato slices and put them in a large beaker, add 1000 parts of sterile water, boil for 20 minutes, filter the filtrate, add 10 parts of lactose, NH 4 NO 3 1 copy, KH 2 PO 4 1.2, MgSO 4 ·7H 2 0.1 part, 0.01 part of bromothymol blue, after mixing uniformly, adjust the pH value to be 7.0 to obtain the culture solution, and the culture solution was sterilized at 121° C. for 30 min to obtain the detection medium of Flammulina velutipes bacterial classification;

[0018] After cooling, place the obtained culture medium in a petri dish, inoculate the mycelium to be tested into the detection medium of Flammulina velutipes strains in a clean bench, and shake and culture at 24°C and 120r / min for 9 days;

[0019] Observe the decolorization of the culture medium. Those that can decolorize the medium are active strains, and those that make the medium color lighter or unchanged are degenerated o...

Embodiment 3

[0021] Wash and peel the fresh potatoes and cut them into thin slices. Weigh 250 parts of potato slices and put them in a large beaker. Add 1000 parts of sterile water, boil for 20 minutes, filter and take the filtrate, and add 30 parts of lactose and NH to the filtrate. 4 NO 3 2.5 parts, KH 2 PO 4 2 parts, MgSO 4 ·7H 2 1.5 parts of O, 0.5 part of bromothymol blue, after mixing uniformly, the pH value is adjusted to 7.1 to obtain a culture solution, and the culture solution is sterilized at 121° C. for 30 min to obtain the detection medium of Flammulina velutipes strains;

[0022] After cooling, place the obtained medium in a petri dish, inoculate the mycelium to be tested into the detection medium of Flammulina velutipes strains in a super-clean workbench, and shake and cultivate at 24°C and 120r / min for 10 days;

[0023] Observe the decolorization of the culture medium. Those that can decolorize the medium are active strains, and those that make the medium color lighte...

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Abstract

The invention relates to a needle mushroom strain detection culture medium and a usage method thereof, and belongs to the technical field of edible fungi strain degeneration detection. The invention aims at solving the technical problem of providing a needle mushroom strain activity degradation detection method which has the advantages of short detection period and high accuracy and a detection culture medium formula. The technical scheme is as follows: the needle mushroom strain detection culture medium comprises the following raw materials in parts by weight: 100-250 parts of potatoes, 10-30 parts of lactose, 1-3 parts of NH4NO3, 1-3 parts of KH2PO4, 0.1-1.5 parts of MgSO4.7H2O and 0.01-0.5 part of bromothymol blue. The needle mushroom strain detection culture medium disclosed by the invention can be used for culturing the strains to be detected; and according to the invention, the strains with good activity can decolorize the culture medium, and degraded strains or inactivated strains enable the color of the culture medium to be shallow or unchanged.

Description

technical field [0001] The invention discloses a culture medium for detecting Flammulina velutipes strains and a method for using the same, belonging to the technical field of edible fungus strain degradation detection. Background technique [0002] Flammulina velutipes is famous all over the world for its tenderness, crisp stalk, rich nutrition, tangy fragrance and delicious taste, and is deeply loved by the public. Due to the expansion of market demand, the industrial cultivation of Flammulina velutipes has developed rapidly in recent years, and a series of problems have also arisen, among which the degradation of strain activity is one of the most influential problems. During the continuous passage of Flammulina velutipes strains in PDA medium, the activity of the strains will be degraded. If the low-activity strains are not found until the fruiting body is formed in this continuous production process, serious economic losses will be caused. . [0003] At present, there...

Claims

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Application Information

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IPC IPC(8): C05G1/00
Inventor 颜丙强巩玉辉陈翠翠宋芳芳张宁
Owner SHANXI JIAQIN AGRI
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