Novel peony tissue culture rooting method

Abstract

The invention discloses a novel peony tissue culture rooting method. Buds of pea green, island kam and yellow peonies are used as explants; under a germfree condition, the buds are disinfected by a three-step disinfection method; tissue culture rooting seedlings are obtained by three steps of constructing a germfree strain, performing subculture multiplication culture and performing root induction culture; and the rooting rates are respectively that the pea green peony is 50.0-66.7 percent, the island kam peony is 33.3-86.7 percent and the yellow peony is 31.4-73.5 percent.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a new rooting method of peony tissue culture. Background technique [0002] peony( Paeonia suffruticosa Andr ) for Paeoniaceae ( Paeoniaceae ) Paeonia ( Paeonia ) Perennial deep-rooted deciduous shrubs with alternate leaves and long stalks. The flowers are unisexual or bisexual, terminal, and the flowering period is April to May. Follicles, cracked when ripe, contain 5-15 seeds, irregular round, brown or black. Peonies are native to the Qinling Mountains and Daba Mountains in the west of my country, and have been cultivated for more than 1,600 years. Peonies grown all over the world are introduced into China. Peony likes sunshine, is cold-resistant, loves cool environment, does not tolerate high temperature, and has high requirements on soil conditions. It is suitable for growth in semi-dry and semi-humid loose, fertile, well-drained sandy soil. The roots of p...

AI Technical Summary

Problems solved by technology

The key to obtaining peony tissue culture plants is rooting and seedling hardening and transplanting, especially rooting technology. The current tissue culture rooting technology of peony shows low rooting rate, poor rooting quality, and difficult to obtain regenerated plants. The biggest bottleneck in the development of peony cultivation technology. The key to rooting cultivation is the medium. Commonly used basic mediums include MS, LP, improved MS, 1 / 2MS and improved WPM. IBA is effective for rooting some peony varieties, and NAA or IAA are also used. In the report of induced rooting, the rooting effect is not very satisfactory. In the retrieved literature, the rooting rate of "Wei Zi" is about 20%, that of "Luoyang Red" is 38.6%, and that of "Oolong Pengsheng" is 33.3%. "Linghua Zhanlu" is 47.9%, "Doulu" has not obtained rooted seedlings, and there are no reports on tissue culture rooted seedlings for rare varieties such as Haihuang and Daojin.

Existing studies have shown that the rooting effect of the same culture method and culture medium is quite different for different peony strains or varieties, and it is basically impossible to obtain ideal tissue culture rooting seedlings for multiple peony strains or varieties at the same time. Therefore, to obtain tissue culture rooted seedlings for different peony strains or varieties, only different methods and media can be selected respectively.

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