Novel peony tissue culture rooting method

A technique of tissue culture and a new method, applied in the field of plant tissue culture, can solve problems such as unsatisfactory rooting effect, difficulty in obtaining regenerated plants, poor rooting quality, etc., achieve obvious rooting effect, enhance the transportation and accumulation of dry matter, and grow The effect of high flower rate

Active Publication Date: 2013-09-04
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The key to obtaining peony tissue culture plants is rooting and seedling hardening and transplanting, especially rooting technology. The current tissue culture rooting technology of peony shows low rooting rate, poor rooting quality, and difficult to obtain regenerated plants. The biggest bottleneck in the development of peony cultivation technology. The key to rooting cultivation is the medium. Commonly used basic mediums include MS, LP, improved MS, 1 / 2MS and improved WPM. IBA is effective for rooting some peony varieties, and NAA or IAA are also used. In the report of induced rooting, the rooting effect is not very satisfactory. In the retrieved literature, the rooting rate of "Wei Zi" is about 20%, that of "Luoyang Red" is 38.6%, and that of "Oolong Pengsheng" is 33.3%. "Linghua Zhanlu" is 47.9%, "Doulu" has not obtained rooted seedlings, and there are no reports on tissue culture rooted seedlings for rare varieties such as Haihuang and Daojin.
Existing studies have shown that the rooting effect of the same culture method and culture medium is quite different for different peony strains or varieties, and it is basically impossible to obtain ideal tissue culture rooting seedlings for multiple peony strains or varieties at the same time. Therefore, to obtain tissue culture rooted seedlings for different peony strains or varieties, only different methods and media can be selected respectively.

Method used

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  • Novel peony tissue culture rooting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Establishment of sterile strains

[0028] Take the full scale buds from the mother plants of Doulu, Daojin, and Haihuang peony, remove the outermost 1-2 layers of scales, scrub with soapy water, then rinse with tap water, and remove the scale buds under aseptic conditions. Put it into a sterile triangular flask, and use a three-step disinfection method to disinfect the scale buds: the first step is to disinfect with 75% ethanol for 45 seconds, the second step is to disinfect with 1% sodium hypochlorite for 10 minutes, take it out and rinse it with sterile water, The third step is to disinfect with 0.5% sodium hypochlorite for 15 minutes, take it out and rinse it with sterile water. During the whole disinfection process, shake the triangular flask constantly to make the disinfectant fully contact with the buds. After disinfection, take it out and place it on sterile filter paper. The remaining scales were peeled off with sterile tweezers, and the scale buds of the thr...

Embodiment 2

[0036] 1. Establishment of sterile strains

[0037] Take the full scale buds from the mother plants of Doulu, Daojin, and Haihuang peony, remove the outermost 1-2 layers of scales, scrub with soapy water, then rinse with tap water, and remove the scale buds under aseptic conditions. Put it into a sterile triangular flask, and adopt a three-step disinfection method to disinfect the scale buds: the first step is to sterilize with 75% ethanol for 60 seconds, the second step is to sterilize with 1% sodium hypochlorite for 8 minutes, take it out and rinse it with sterile water, The third step is to sterilize with 0.5% sodium hypochlorite for 20 minutes, take it out and rinse it with sterile water. During the whole disinfection process, shake the triangular flask constantly to make the disinfectant fully contact with the buds. After disinfection, take it out and place it on a sterile filter paper. The remaining scales were peeled off with sterile tweezers, and the scale buds of the ...

Embodiment 3

[0045] 1. Establishment of sterile strains

[0046] Take the full scale buds from the mother plants of Doulu, Daojin, and Haihuang peony, remove the outermost 1-2 layers of scales, scrub with soapy water, then rinse with tap water, and remove the scale buds under aseptic conditions. Put it into a sterile triangular flask, and use a three-step disinfection method to disinfect the scale buds: the first step is to disinfect with 75% ethanol for 30 seconds, the second step is to disinfect with 1% sodium hypochlorite for 15 minutes, take it out and rinse it with sterile water The third step is to sterilize with 0.5% sodium hypochlorite for 10 minutes, take it out and rinse it with sterile water. During the whole disinfection process, shake the triangular flask constantly to make the disinfectant fully contact with the scale buds. After disinfection, take it out and place it on sterile filter paper. The remaining scales were peeled off with sterile tweezers, and the scale buds of th...

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PUM

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Abstract

The invention discloses a novel peony tissue culture rooting method. Buds of pea green, island kam and yellow peonies are used as explants; under a germfree condition, the buds are disinfected by a three-step disinfection method; tissue culture rooting seedlings are obtained by three steps of constructing a germfree strain, performing subculture multiplication culture and performing root induction culture; and the rooting rates are respectively that the pea green peony is 50.0-66.7 percent, the island kam peony is 33.3-86.7 percent and the yellow peony is 31.4-73.5 percent.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a new rooting method of peony tissue culture. Background technique [0002] peony( Paeonia suffruticosa Andr ) for Paeoniaceae ( Paeoniaceae ) Paeonia ( Paeonia ) Perennial deep-rooted deciduous shrubs with alternate leaves and long stalks. The flowers are unisexual or bisexual, terminal, and the flowering period is April to May. Follicles, cracked when ripe, contain 5-15 seeds, irregular round, brown or black. Peonies are native to the Qinling Mountains and Daba Mountains in the west of my country, and have been cultivated for more than 1,600 years. Peonies grown all over the world are introduced into China. Peony likes sunshine, is cold-resistant, loves cool environment, does not tolerate high temperature, and has high requirements on soil conditions. It is suitable for growth in semi-dry and semi-humid loose, fertile, well-drained sandy soil. The roots of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李艳敏孟月娥王利民赵秀山王慧娟张淑玲张和臣董晓宇符真珠杜君
Owner HENAN ACAD OF AGRI SCI
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