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Cordyceps militaris, cultivation method and separation method

A cultivation method, the technology of Cordyceps militaris, which is applied in the field of microorganisms, can solve the problems of not being able to meet the needs of medicinal use, and the content of mycocin is not high.

Inactive Publication Date: 2014-07-16
贵州安顺金黔虫草有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of cordycepin in the artificially planted fruiting bodies of Cordyceps militaris is not high, generally below 0.5% (accounting for the dry weight of fruiting bodies), which is far from meeting the needs of medicinal use and market demand.

Method used

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  • Cordyceps militaris, cultivation method and separation method
  • Cordyceps militaris, cultivation method and separation method
  • Cordyceps militaris, cultivation method and separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The surface of the harvested Cordyceps militaris fruiting body was sterilized with 75% ethanol, and then the fertile part of the subunit was cut with a sterile scalpel, and a small piece of tissue or tissue with asci shell was inoculated on a Sabouraud plate, 24 Cultivate at -26°C for 4-5 days, transfer to Sabouraud's slant, culture at 24°C-26°C for 6-7 days, take out and store at 4°C the obtained Cordycepes militaris GZU-19 (Cordycepes militaris GZU-19) CCTCC NO:M2013056.

[0032] To prepare Sabouraud’s medium, first take various raw materials of Sabouraud’s medium: 5 grams of maltose, 20 grams of glucose, 10 grams of peptone, 5 grams of yeast extract, 15 grams of agar, 0.5 grams of magnesium sulfate, 0.5 grams of ferrous sulfate, Potassium chloride 1 gram; Then maltose, glucose, peptone, yeast extract, agar, magnesium sulfate, ferrous sulfate, potassium chloride were added to 600ml of distilled water successively to prepare a mixed solution, and the mixed solution was ...

Embodiment 2

[0079] The difference from Example 1 is: Sabouraud medium: 10 grams of maltose, 10 grams of glucose, 8 grams of peptone, 4 grams of yeast extract, 15 grams of agar, 0.1 grams of magnesium sulfate, 0.1 grams of ferrous sulfate, and 0.5 grams of potassium chloride. gram.

Embodiment 3

[0081] The difference with Example 1 is: Sabouraud medium: 20 grams of maltose, 30 grams of glucose, 23 grams of peptone, 15 grams of yeast extract, 20 grams of agar, 1 gram of magnesium sulfate, 1 gram of ferrous sulfate, potassium chloride 2 gram.

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Abstract

The invention relates to the field of microorganisms, in particular to cordyceps militaris paecilomyces varioti, a cultivation method and a separation method. The collection number of the cordyceps militaris GZU-19 is CCTCC (China Center for Type Culture Collection) NO (Number): M2013056. The cultivation method of the cordyceps militaris comprises the steps of activating a culture: inoculating the collected cordyceps militaris onto an oblique surface of a Somogyi culture medium for activation cultivation, dibbling the activated cordyceps militaris onto a flat plate of the Somogyi culture medium for morphological identification growth cultivation, and obtaining an aerial mycelium. The cordyceps militaris paecilomyces varioti, the cultivation method and the separation method have higher cordycepin synthesis ability, and a foundation is laid for the fermentation industry of artificial cultivation fruit bodies and microorganisms of high-yield cordycepin.

Description

technical field [0001] The present invention relates to the field of microorganisms, in particular to Cordycepes militaris GZU-19 (Cordycepes militaris GZU-19), a cultivation method and an isolation method. [0002] Cordycepes militaris GZU-19 (Cordycepes militaris GZU-19), the preservation number is CCTCC NO: M2013056, preserved in the China Center for Type Culture Collection (CCTCC), address: China. Wuhan. Wuhan University, postcode: 430072, preservation time is 2013 February 4th. Background technique [0003] Cordyceps militaris is also called Cordyceps militaris and Cordyceps militaris. The anamorph of Cordyceps militaris is Paecilomyces militaris, and the anamorph differs greatly in terms of colony characteristics, ability to form sexual fruiting bodies, and sporulation structure at the level of monosperm strains. Cordyceps militaris is rich in active substances, such as cordycepin, polysaccharides, nucleosides, carotenoids, etc., which have a wide range of physiologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 熊艳
Owner 贵州安顺金黔虫草有限公司