A method for production of a human body biological dressing for covering burn wounds

Abstract

The present invention provides a method for production of a human body biological dressing for covering burn wounds. Through methods of biology, biochemistry, etc., antigen extraction for reducing immunogenicity is carried out to allograft, dermis and epidermis is retained, and the clinical effect of the obtained biological dressing is far better than the allograft. The method of the present invention includes the following sequential steps: immersion in a glutaraldehyde solution; segmentation; immersion in purified water; immersion in a polysorbate 80 solution; immersion in purified water; immersion in an amino acid solution; immersion in purified water; immersion in a PBS buffer solution; repair and recondition; immersion in a sodium chloride solution; and packaging and irradiation. The resulting product of the present invention can be used for purifying and cleaning wounds, promoting the growth of granulation tissues to cover granulation wounds, ulcers and bed sores, and shortening the healing time.

Description

technical field [0001] The invention relates to a preparation method of a burn wound wound covering material, in particular to a preparation method of a human biological dressing which is used for covering the burn wound surface with the antigen-removed allogeneic skin and reduced immunogenicity. Background technique [0002] Burns are the most common skin wounds in peacetime and wartime, and all kinds of damage caused by burns are related to the destruction and loss of skin barrier function. Such as: increased metabolism, decreased body temperature, excessive loss of water, a large loss of protein, and disorders of the endocrine and immune systems. Therefore, it is particularly important to cover wounds with dressings as a temporary body surface protection barrier to promote wound healing, especially for large area burns. Biomaterials have long been found to aid in the healing of skin wounds. With the continuous deepening of research on wound healing, people gradually rea...

AI Technical Summary

Problems solved by technology

[0004] Traditional dressings such as gauze and cotton pads have the following disadvantages: ① cannot keep the wound moist and delay wound healing; ② dressing fibers are easy to fall off, causing foreign body reactions and affecting healing; ③ wound granulation tissue is easy to grow into the mesh of the dressing, and the Can cause pain; ④ When the dressing is soaked, pathogens can easily pass through; ⑤ When changing the dressing, it is easy to damage the new tissue; ⑥ The workload of changing the dressing is heavy

Foam-type synthetic dressings such as Allevyn, Ixralo and other products have large pores, and the wound granulation tissue is easy to grow in, making it difficult to remove the membrane and cause re-trauma, and it is easy to be contaminated by bacteria, and it is easy to leave debris on the wound

Spray-type synthetic dressings such as Hydron, Aeroplast and other products have the following disadvantages: poor adhesion and tensile strength; poor moisture retention, large wound water evaporation; water solubility of the spray film can make it softened by wound exudate and keep for a short time ; No infection control effect, used to contaminate the wound surface prone to submembranous infection

However, the existing allogeneic skin has clinical problems such as high storage conditions, antigenicity, space occupation, and susceptibility to pathogenic microorganisms.

In particular, the obvious immune rejection caused by its antigenicity usually dissolves and falls off in about 2 to 3 weeks, and the wound surface is exposed, which affects the primary healing

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