Swine-derived component real-time fluorescent PCR detection method and primers

Abstract

The invention discloses a swine-derived component real-time fluorescent PCR (polymerase chain reaction) detection method, which comprises the steps of: designing swine specific primers, external primers and internal primers of an internal reference GCG-R gene; conducting PCR amplification on a gene fragment with a length of 270bp in the internal reference GCG-R gene; diluting the gene fragment by 10 times with DEPC (diethypyrocarbonate) water; extracting DNA of a sample; employing the swine specific primers, the internal primers and the diluted gene fragment to carry out swine-derived component real-time fluorescent PCR detection on the DNA. The invention also discloses the primers adopted by the swine-derived component real-time fluorescent PCR detection method. The primers include the swine specific primers, the external primers and the internal primers. By introducing an internal reference gene GCG gene template and its primers, the method can accurately, effectively, and rapidly detect swine-derived components, and the detection result is accurate and reliable. Compared with the current identification technologies, the method provided in the invention can realize accurate and fast identification that whether meat food contains a swine-derived component.

Description

technical field [0001] The invention relates to identification of animal species and animal-derived components, in particular to the detection of pig-derived components, and in particular to a real-time fluorescent PCR detection method and primers for pig-derived components. Background technique [0002] With the increasing demand for meat products in modern society, the adulteration and adulteration of meat products, the spread of animal diseases, religious taboos on meat products, and the protection of rare animals have attracted the attention of the current society. The identification and analysis technology of livestock and poultry muscle species is the scientific basis for combating violations of laws and regulations, and it is also a powerful guarantee for the protection of rare animals. [0003] Currently, molecular biology methods are the most accurate and sensitive methods for the detection of animal-derived components. At home and abroad, detection kits for common...

AI Technical Summary

Problems solved by technology

[0005] Using the traditional real-time fluorescent PCR detection method to detect pig-derived components has the following problems: because additives (such as sodium chloride, oil, etc.) In the process, it will inevitably enter the DNA solution, which may inhibit the PCR reaction process, reduce the PCR amplification efficiency, cause false negative results, affect the detection judgment, and cause the detection results to be inaccurate

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