Leuconostoc mesenteroides strain

A technology of Leuconostoc enterococcus and strains, which is applied in the direction of bacteria, fungicides, food preservation, etc., can solve the problems of consumer anxiety, and achieve the effect of maintaining color and inhibiting browning

Active Publication Date: 2015-03-11
DONGGUAN AGRI SCI RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, reports of sour lychee and lime mango appeared in newspapers and on the Internet, which constantly aroused uneasiness among consumers

Method used

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  • Leuconostoc mesenteroides strain
  • Leuconostoc mesenteroides strain
  • Leuconostoc mesenteroides strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The inventor collected healthy, pest-free and mechanically-damaged longan fruits from the longan fruits produced in the orchard of Dongguan Agricultural Science Research Center on September 11, 2011, and peeled and broken 150g of the fruits in a previously prepared aseptic Naturally fermented in a flask, the pulp was broken and fermented together with the peel and core in an anaerobic incubator at 37°C for 2 days. After 2 days of fermentation, pipette the fermented liquid and dilute it by serial concentration gradient dilution method, and dilute the fermented liquid to 10 with sterile water. -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 A total of 6 gradient dilutions were repeated three times. take 10 -4 、10 -6 For two gradient dilutions, draw 0.2-0.3mL and add it to the solidified MRS medium, and then quickly spread the fermentation broth evenly with a spreading stick. The plate was then placed upside down in an anaerobic incubator at 37°C. After the colonies grow ou...

Embodiment 2

[0098] S1. Cultivation of fresh-keeping bacteria:

[0099] S11. Activation of strains: Put the fresh-keeping strains into 20mL MRS liquid medium with a 2% inoculation amount from a glycerol tube, and place them in a 37°C incubator for static activation for 20 hours;

[0100] MRS liquid medium ingredients: 1L distilled water, peptone 10g, beef extract 10g, yeast extract 5g, dipotassium hydrogen phosphate 2g, diammonium citrate 2g, sodium acetate 5g, glucose 20g, magnesium sulfate heptahydrate 0.58g, manganese sulfate tetrahydrate 0.25g, Tween 801mL, pH6.2~6.4, sterilized at 121℃ for 15min.

[0101] S12. Expanded cultivation: Inoculate the activated seed solution into 1000mL MRS liquid medium at an inoculum size of 5%, and culture it statically in a 37°C incubator for 20 hours;

[0102] S13. Preparation of bacterial cell suspension: after the fermentation is completed, the fermentation liquid is centrifuged at 8500r / min and 4°C for 15 minutes to collect the bacterial cells, was...

Embodiment 3

[0106] Embodiment 3: preservative agent application experiment

[0107] On the premise of confirming that the strain has a fresh-keeping effect, the present invention uses the cell suspension of Leuconostoc enterococci strains as an effective active ingredient, and at the same time determines a reasonable matching of biological substances to compound a safe and efficient biological preservative . Described antistaling bacteria agent comprises each component of following mass percentage content: 1% chitosan, 2% citric acid, 0.85% sodium chloride, 0.5% kojic acid and 2% sodium lactate, surplus is water; The 100% system composed of components was added with the bacterium suspension of the strain so that the bacterium concentration in the system was 2.9×10 8 CFU / mL, to prepare a preservative.

[0108] S1. Cultivation of fresh-keeping bacteria:

[0109] S11. Activation of bacterial strains: Put the glycerol strain of Leuconostoc Mesenteroides dgnkzx002 into 20mL MRS liquid mediu...

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Abstract

The invention relates to the field of biological technology, and discloses an application of a leuconostoc mesenteroides strain, wherein the strain is preserved in China Center for Type Culture Collection (CCTCC), the preservation date is Nov. 26, 2012, and the preservation number is CCTCC M 2012479. The strain has good inhibition effect on peronophythora litchi and / or anthracnose pathogen, can substantially reduce activities of polyphenol oxidase (PPO) and / or peroxidase (POD) of lichee or longan, and keep superoxide dismutase (SOD) activity of pericarps. Therefore, the strain has important application value in fresh-keeping aspect of fruits such as lichee, longan and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, to a strain of Leuconostoc enterococcus. Background technique [0002] From harvesting to consumption by consumers, fresh fruits and vegetables suffer from self-respiration and pathogenic organisms infection. Every year, a large number of fruits and vegetables rot and lose during transportation and storage after harvesting. A survey by the National Agricultural Products Preservation Engineering Technology Research Center found that the current annual loss rate of fruits and vegetables from the field to the dining table in my country is as high as 25-30%, with an annual loss of nearly 80 billion yuan. Therefore, it is urgent to further carry out research and development of fruit and vegetable preservation technology to ensure the realization of added value of fruit and vegetable products and the full utilization of resources. [0003] At the same time, with the improvement of livin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B7/155A01N63/02A01P3/00C12R1/01
Inventor 罗诗胡文锋马锞徐匆张长勇罗华建黄应维胡珊范妍李艳芳
Owner DONGGUAN AGRI SCI RES CENT
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