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Method for extracting and preparing protease from water lettuce roots

A technology of water lotus and protease, which is applied in the field of extraction and preparation of protease, can solve the problems of low added value, low utilization rate of water lotus, large development cost, etc., and achieves low production cost, convenient large-scale production, and equipment investment. less effect

Inactive Publication Date: 2013-09-25
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the resource development of water succulents needs to invest a large amount of development costs, and the utilization rate of succulents is low, and the added value produced is low

Method used

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  • Method for extracting and preparing protease from water lettuce roots
  • Method for extracting and preparing protease from water lettuce roots
  • Method for extracting and preparing protease from water lettuce roots

Examples

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Embodiment 1

[0028] Such as figure 1 Shown in the flow chart of the present invention, the concrete steps of the method for extracting and preparing protease from water plantain root of the present invention are as follows:

[0029] (1) Prepare fresh water floating lotus, wash it with water, cut off the root of the water floating lotus, and weigh 100 grams of the water floating lotus root;

[0030] (2) Add 100 ml of water or phosphate buffer solution to the weighed water lotus root for homogenization and mixing, and after leaching for 1 hour, separate the supernatant and sediment of the homogenization by a centrifuge. The clear is the protease extract;

[0031] (3) Take the protease extract, add ethanol to a final concentration of 80%, or add acetone to a final concentration of 70% to 90%, let it stand for 1 hour, and separate the supernatant and precipitate by a centrifuge, and the precipitate is protease;

[0032] (4) Preserving the protease precipitate obtained in the step (3) through...

Embodiment 2

[0035] After the protease extract obtained in step (2) of the above-mentioned embodiment 1, the protease precipitate obtained in step (3) and the protease powder obtained in step (4) were treated by casein hydrolysis, and then treated with 0.1% gelatin Polyacrylamide electrophoresis gel autographic method was used to measure the enzymatic activity of protease, and the results were as follows:

[0036] Please refer to figure 2 , in addition to extracting and preparing protease from the root of water lettuce, as a comparison, other different biological materials were selected for the extraction and preparation of protease, including: apple, dragon fruit, mushroom, orange, pear, longan, banana, pineapple And papaya, wherein papaya is a commonly used protease extraction raw material, through the comparison of the enzyme activity of protease extracted from different biological materials, it is shown that the extraction amount and enzyme activity of protease extracted from water pl...

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Abstract

The invention discloses a method for extracting and preparing a protease from water lettuce roots. The method comprises the following steps of: mincing and homogenating fresh water lettuce roots and extracting by using water or a phosphate buffer solution; precipitating the extract by using an organic solvent and separating the protease, and then drying the protease, thereby obtaining high-activity protease powder. The method disclosed by the invention is used for producing high-activity protease from water lettuce; and therefore, the method is capable of changing wastes into things of value, energy-saving and environment-friendly, simple to operate, low in product production cost and convenient for large-scale production.

Description

technical field [0001] The invention relates to a technology for extracting and preparing protease, in particular to a method for extracting and preparing protease from water plantain root. Background technique [0002] The Chinese name of water floating lotus is Eichhornia crassipes, and its English name is water hyacinth. The water hyacinth of monocotyledonous plant class, commelina order, hyacinthaceae, water hyacinth genus is a kind of water floating plant with strong reproductive ability. In recent years, due to the deteriorating climate and environment, water lilies have grown and flooded all over the world and in South China, East China, Central China and North China. They are regarded as harmful organisms that pollute the environment and plague most southern cities in my country. It has caused serious environmental and social problems, including blocking waterways, affecting shipping and excretion; restricting water flow, destroying aquatic ecological environment; de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50
Inventor 李海航倪贺陈方媚范晓敏
Owner SOUTH CHINA NORMAL UNIVERSITY
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