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Method for simultaneously detecting five medicaments in water

A detection method and water detection technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of non-negligible mixed toxicity and chronic toxicity effects, incomplete absorption and utilization of drugs, and low antibiotic content, etc., to achieve selectivity Strong, easy to handle, reproducible results

Inactive Publication Date: 2013-09-25
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] These common drugs are generally not fully absorbed and utilized, and the drugs or their metabolites will enter the environment through urine, feces, etc.
However, the existing treatment technologies in sewage treatment plants cannot or basically cannot effectively remove them. The research results show that the content of lactam antibiotics in environmental water samples is very small, only ng / L or μg / L level
Although the residual drugs at the concentration range from ng / L to μg / L in the environment are not enough to cause acute or subacute toxic effects on organisms, their mixed toxic and chronic toxic effects cannot be ignored

Method used

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  • Method for simultaneously detecting five medicaments in water
  • Method for simultaneously detecting five medicaments in water
  • Method for simultaneously detecting five medicaments in water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: recovery rate experimental result

[0054] Take 1000ml of ultrapure water in a brown glass bottle, add 1ml of 100μg / L mixed standard stock solution of 5 common drugs to each bottle of water sample, mix well and add a small amount of hydrochloric acid to adjust the pH of the water sample to about 3.0. Utilize 6mL chromatographically pure acetone, 6mL chromatographically pure methanol, 6mL 0.1% (m / v) formic acid aqueous solution containing 5mmol / L ammonium acetate, and 15-20mL ultrapure water to activate the HLB (500mg, 6cc) solid phase extraction column of Waters Company successively , control the flow rate to less than 1mL / min;

[0055] (2) Concentration and enrichment

[0056] After the enrichment is completed, dry the HLB solid-phase extraction column under nitrogen protection for 20 minutes, use 10mL of 5% ammonia water methanol solution to elute the HLB column, control the flow rate to less than 1mL / min, collect the eluate in a 10mL conical bottom gla...

Embodiment 2

[0063] Example 2: The recovery results of water samples from Beijing Olympic Park.

[0064] Take 1000ml of river water samples from Beijing Olympic Park in a brown glass bottle, and carry out the standard addition experiment according to the method in Example 1, while taking 1000ml of river water samples without adding standards, as a blank control.

[0065] The results of the spiked water samples were brought into the standard curve equation, and the concentrations of five common drugs in the water samples to be tested were finally obtained by calculation. The recovery rate was calculated, and the results are shown in Table 2.

Embodiment 3

[0066] Example 3: Measurement results of actual water samples in Wangyanggou, Shijiazhuang, Hebei.

[0067] 1000mL water samples from Ziyahe Wangyanggou (38°18′28″N, 114°24′45″E) were subjected to negative pressure suction filtration through a glass fiber membrane (Whatman, 0.45μm), and the filtrate was subjected to a 6mL chromatographic Pure acetone, 6mL chromatographically pure methanol, 6mL 0.1% (m / v) formic acid aqueous solution containing 5mmol / L ammonium acetate, 15-20mL ultrapure water activate the HLB cartridge, and control the flow rate at 2-5mL / min. Vacuum dry for 1 min after the enrichment, 10mL of 5% ammonia water methanol solution was used to elute the HLB column, the eluate was collected in a 10mL pointed-bottom glass colorimetric tube, and the eluate was slowly blown under nitrogen flow until it was nearly dry, and 400 μL of chromatographic pure The residue was dissolved in acetonitrile, vortexed, and tested.

[0068] The concentrations of five common drugs in ...

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Abstract

The invention discloses a method for simultaneously detecting the medicaments such as furazolidone, diazepam, trimethoprim, acetaminophen and ibuprofen in water. The method comprises the following steps of: filtering a water sample to remove suspended matter; adjusting the pH of the water sample to be 2 to 4; activating hydrophile-lipophile balance (HLB) solid phase extraction column by using acetone, methanol, ammonium acetate-containing formic acid aqueous solution and ultrapure water in sequence; after the enrichment is finished, drying the HLB solid phase extraction column under the protection of nitrogen and eluting the HLB solid phase extraction column; collecting eluant, and drying the eluant by blowing a nitrogen flow; adding acetonitrile to dissolve the residue; and quantitatively detecting the concentration of the five medicaments. By the method, the water sample pretreatment is environment-friendly, easy to operate, large in enrichment factor, and high in reproducibility; and the content of the five common medicaments in a water environment can be analyzed quickly and accurately.

Description

technical field [0001] The invention belongs to the technical field of detection of water environmental pollutants, and in particular relates to a detection method for five trace common drugs in water bodies, and more specifically relates to a method for detecting trace amounts of furazolidone, diazepam, Methods for the detection of trimethoprim, acetaminophen, and ibuprofen. Background technique [0002] Five common drugs (furazolidone, diazepam, trimethoprim, acetaminophen, and ibuprofen) play vital roles in daily life. Furazolidone, also known as furazolidone, has the advantages of high curative effect and broad antibacterial spectrum, and is used in the prevention and treatment of bacterial diseases in aquatic animals in aquatic product farming. However, studies have shown that furazolidone and its metabolites in vivo have mutagenic carcinogenic effects and other toxic and side effects. Diazepam is a commonly used sedative-hypnotic drug in clinical practice. It has sed...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/08
Inventor 许其功薛成张慧张媛高如泰梁琼曹金玲
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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