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Low temperature storage method and low temperature storage container

A technology of cryopreservation and containers, applied in the fields of food preservation, preservation of human or animal bodies, biochemical equipment and methods, etc.

Inactive Publication Date: 2013-09-25
MITSUYA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, in the pressurized freezing method in the prior art, it is necessary to prepare a large-scale pressurized device that generates high pressure

Method used

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  • Low temperature storage method and low temperature storage container
  • Low temperature storage method and low temperature storage container
  • Low temperature storage method and low temperature storage container

Examples

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no. 1 example

[0051] Hereinafter, the cryopreservation method and the cryopreservation container according to the first embodiment of the present invention will be described with reference to the accompanying drawings.

[0052] Such as figure 1 As shown, a cryopreservation container 10 containing an object to be stored A is suspended by a hanging rod 2 via a hanger 1 in a contact solvent 4 accommodated in a freezing container forming tank 3 .

[0053] The freezing container forming tank 3 accommodates a contact refrigerant 4 such as ethanol brine, and is set in a freezing chamber (not shown) to be cooled. Save object object A to accommodate in such as figure 2 The state inside the cryopreservation container 10 shown is suspended in contact with the refrigerant 4 .

[0054] Such as figure 2 As shown, the cryopreservation container 10 has a triple structure including an outer container 11 (container), an inner bag 12 , an inner bag 13 , and a spindle 14 . The upper part of the outer con...

no. 2 example

[0108] Hereinafter, the cryopreservation method and the cryopreservation container in the second embodiment of the present invention will be described with reference to the drawings.

[0109] Such as image 3 As shown, the cryopreservation container 20 includes an outer container 21 , a first bag 22 and a second bag 23 . Although not shown, the top of the outer container 21 is fixed on the hook 1 (see figure 1 )superior.

[0110] The outer container 21 is, for example, a resin such as polyethylene terephthalate (PET) or a container made of metal such as iron. The first bag 22 and the second bag 23 are flexible, and are made of resin such as polyethylene, for example.

[0111] The first closed space inside the first bag 22 is filled with liquid C having a higher compressibility than liquid D and having a lower freezing point than liquid D. The liquid C that is filled in the first bag 22 is the same as the liquid C that is filled in the above-mentioned middle bag 22, and is ...

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Abstract

A low temperature storage container (10) is sealed, after a stretchable middle bag (12) that makes a subject to be stored (A) exist therein and is filled with a liquid (C) having the compression rate larger than a liquid (D) and having the freezing point temperature lower than the liquid (D) is put into an outer container (11), and a space between the outer container (11) and the middle bag (12) is filled with the liquid (D) with the volume which is increased when freezing. Then, the low temperature storage container (10) is cooled, and the subject to be stored (A) is stored at the storage temperature that is the temperature between the freezing points of the liquid (C) and the liquid (D) and is lower than the freezing point temperature at atmospheric pressure of a liquid in the subject to be stored (A).

Description

technical field [0001] The present invention relates to a cryopreservation method for preserving objects to be preserved, such as food, organs, and cells, at a low temperature, and to a cryopreservation container used in the cryopreservation method. Background technique [0002] Generally speaking, the lower the storage temperature of food, organs, blood and other organic substances, the more it can inhibit the activity and chemical reaction of bacteria or enzymes, and the longer it can be stored. Therefore, when storing an object to be stored for a long period of time, it is often frozen, solidified in an amorphous state, and frozen. [0003] However, in cryopreservation, tissue is damaged due to volume expansion due to the formation of ice crystals. Therefore, a method of preserving an object to be preserved while suppressing the formation of ice crystals at the lowest possible low temperature has been sought. [0004] In addition, people are also studying ways to add an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/37A01N1/02A23L3/36B65D81/18C12M1/02
CPCC12M45/22A01N1/0252A23L3/36A23L3/364
Inventor 木野正人清水昭夫
Owner MITSUYA CO LTD
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