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Method for dynamically determining breathing oxygen consumption and hypoxia device for same

A measuring device and body technology, applied in the evaluation of respiratory organs, etc., can solve the problem of high oxygen consumption and achieve the effect of avoiding errors

Inactive Publication Date: 2015-01-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem that the existing hypoxia device uses the visual method to count the respiratory rate of mice, the higher the oxygen consumption in the process of oxygen consumption measurement, the lower the pressure in the oxygen consumption environment, the longer the hypoxic hypoxia time, and the lower the oxygen consumption in the oxygen consumption environment. The lower the medium pressure, the lower the accuracy of the experimental results, etc., the present invention proposes a method to keep the pressure of the hypoxic environment in a constant state during the hypoxic process, and can automatically and dynamically monitor the respiratory frequency, amplitude, and energy consumption of the hypoxic mice. Oxygen and the pressure value of the hypoxic environment, to avoid errors caused by manual monitoring, and suitable for both oxygen consumption and hypoxic experiments, a respiratory oxygen consumption dynamic hypoxia measurement device and its measurement method

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  • Method for dynamically determining breathing oxygen consumption and hypoxia device for same
  • Method for dynamically determining breathing oxygen consumption and hypoxia device for same
  • Method for dynamically determining breathing oxygen consumption and hypoxia device for same

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Experimental program
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Embodiment 1

[0031] Embodiment 1 A respiratory oxygen consumption dynamic hypoxia measuring device according to the present invention includes a device body 1, a hypoxia bottle, and a biological signal acquisition and processing system. The device body 1 is provided with an oxygen consumption capacity tank 11, an oxygen consumption measurement Channel 12, exhaust channel 13, the oxygen consumption capacity tank 11 communicates with the end of the oxygen consumption measurement channel 12; the exhaust channel 13 communicates with the oxygen consumption measurement channel 12; located in the device body The oxygen consumption measurement channel 12 at the tail, the exhaust channel 13 at the head of the device body 1, and the oxygen consumption capacity tank 11 are respectively provided with corresponding vent holes 121, exhaust ports 131 and replacement parts. The transducer connection port 111 is connected to the high-sensitivity tension transducer straight port of the biological signal acqu...

Embodiment 2

[0040] Embodiment 2 utilizes the measuring method that the device of embodiment 2 carries out, comprises the following steps:

[0041] 1) Put the head of the device body upwards, add distilled water from the vent to the water retaining strip and place it horizontally on the table, connect the exhaust port to the three-way valve, connect the transducer connection port of the device body to the biological signal acquisition and processing system The high-sensitivity tension transducer is directly connected to the outlet, and the outlet of the anoxic bottle is connected to the side port of the high-sensitivity tension transducer;

[0042]2) Close the three-way valve at the exhaust port, put mice into the anoxic bottle with soda lime in advance, and cap the bottle tightly;

[0043] 3) The outlet of the hypoxic bottle is connected to the connector of the transducer, and the mouse in the hypoxic bottle breathes and consumes oxygen to produce 0.5cmH 2 O negative pressure, pump the w...

Embodiment 3

[0047] Embodiment 3 is connected good breathing oxygen consumption dynamic hypoxia device and conventional oxygen consumption measuring device according to embodiment 2, adjusts the length of connecting pipeline and makes two kinds of oxygen consumption devices capacity equal, adds an equivalent amount of soda lime (H 3 Ca 2 NaO 4 , purchased from Shanghai Shunqiang Biotechnology Co., Ltd.), set the record acquisition parameters of RM6240 (Chengdu Instrument Factory): time constant DC, filter frequency 30Hz, sensitivity 5ml / s, sampling frequency 800Hz, scan speed 1s / div, low-pass filter 30Hz, and calibrated with a water pressure gauge. 60 mice (purchased from the Animal Center of Zhejiang University, body weight 21.0±27g, half male and half female) were weighed and marked, and two mice of the same gender and weight were randomly selected to form a pair, and one of them was placed in In the hypoxic bottle of the conventional oxygen consumption device, the bottle stopper was t...

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Abstract

The invention discloses a method for dynamically determining breathing oxygen consumption and a hypoxia device for the method for dynamically determining the breathing oxygen consumption. The method includes the following steps that after distilled water is added from an air vent and reaches a weather bar, a tee valve is closed, a mouse is contained in a hypoxia bottle where soda lime is placed in advance, and then a bottle plug is fastened; when the hypoxia bottle generates 0.5cmH2O negative pressure, water flows in an oxygen consumption determining channel enable the pressure to be -0.5cmH2O constantly, and the amount of consumed oxygen is read out; a high-sensitivity tension transducer is used for automatically monitoring the breathing rate, the breathing range and breathing pressure of the laboratory mouse with oxygen deficiency; when a repeated experiment is performed, air in the oxygen consumption determining channel is exhausted and then the operations are repeated; the hypoxia device established according to the determining method comprises a device body, the hypoxia bottle and a biological signal collecting and processing system. The method for dynamically determining the breathing oxygen consumption and the hypoxia device for the method for dynamically determining the breathing oxygen consumption have the advantages that the pressure is constant, due to the fact that the breathing rate, the breathing range, the amount of the consumed oxygen and the pressure value of the hypoxia environment of the mouse with hypoxia are monitored dynamically, errors caused by manual monitoring are avoided, and the method and the hypoxia device are suitable for determination of the consumed oxygen amount and a hypoxia experiment.

Description

technical field [0001] The invention relates to a device for measuring respiratory oxygen consumption and dynamic hypoxia and a measuring method thereof. Background technique [0002] Hypoxia experiment is a routine experiment project in functional experiments and anti-hypoxia protective effect research. The conventional oxygen consumption measurement device consists of 125ml wide-mouth bottle, 50ml measuring cylinder, 25ml fat belly straw and pipeline system. It consists of 125ml wide-mouth bottle and The anoxic hypoxia device composed of a closed bottle stopper has a simple structure and is easy to obtain. It is a necessary experimental tool for hypoxia experiments in the current "National Medical Experiment Planning Textbook for Colleges and Universities". The defect of the conventional oxygen consumption measuring device is that the more the oxygen consumption, the lower the pressure in the hypoxic environment, resulting in systematic errors in the oxygen consumption mea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/08
Inventor 梅汝焕
Owner ZHEJIANG UNIV