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Integrated multifunctional controllable cell control and analysis micro-fluidic chip and application thereof

A microfluidic chip and microfluidic technology, applied in the field of biomedical applications, can solve the problems of difficult integration of high-throughput analysis, micro-analysis and controllable cell manipulation functions, and achieve simple structure, easy fabrication, and simplified operation process. Effect

Active Publication Date: 2014-09-17
HUNAN INSTITUTE OF ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The first purpose of the present invention is to provide an integrated multifunctional controllable cell manipulation and analysis microfluidic chip, which integrates multiple functional structures for cell analysis to solve the high-throughput problem in current cell analysis methods Problems that are difficult to integrate functions such as analysis, microanalysis and controllable manipulation of cells, and promote the further development of this technical field

Method used

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  • Integrated multifunctional controllable cell control and analysis micro-fluidic chip and application thereof
  • Integrated multifunctional controllable cell control and analysis micro-fluidic chip and application thereof
  • Integrated multifunctional controllable cell control and analysis micro-fluidic chip and application thereof

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Effect test

Embodiment 1

[0020] Example 1: Applying the microfluidic chip of the present invention to cell stress analysis.

[0021] (1) The design of the integrated multifunctional controllable cell manipulation and analysis microfluidic chip of the present invention:

[0022] The designed microfluidic chip can conduct orderly manipulation, controllable packaging and formation of cells in the chip, complete the design and functional verification of the above three modules, and then integrate the flow paths and structural characteristics of the three modules into one chip on and connect with the channel. The chip includes a cell orderly manipulation module (asymmetric curved channel), a cell controllable wrapping module (oil phase channels on both sides and a middle water phase channel required for droplet formation), a cell array formation module and a simple laminar flow based diffusion The principle of concentration gradients forms channels.

[0023] (2) Preparation of microfluidic chip:

[0024...

Embodiment 2

[0031] Example 2: The microfluidic chip of the present invention is used for performance analysis of antitumor drugs.

[0032] (1) The design of the integrated multifunctional controllable cell manipulation and analysis microfluidic chip of the present invention:

[0033] The designed microfluidic chip can conduct orderly manipulation, controllable wrapping and formation of cells in the chip, complete the design and functional verification of the above three modules, and then integrate the flow paths and structural characteristics of the three modules into one on-chip and channel-connected. The chip includes a cell orderly manipulation module (asymmetric curved channel), a cell controllable wrapping module (oil phase channels on both sides and a middle water phase channel required for droplet formation), a cell array formation module and a simple laminar flow based diffusion The principle of concentration gradients forms channels.

[0034] (2) Preparation of microfluidic chi...

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Abstract

The invention belongs to the technical field of biomedicine application of micro total analysis systems, and particularly relates to an integrated multifunctional controllable cell control and analysis micro-fluidic chip and application of the chip. The integrated multifunctional controllable cell control and analysis micro-fluidic chip comprises an inertia focusing structure formed by continuous asymmetric bend channels, a laminar flow structure formed by a plurality of branch channels and a joining channel, a micro-fluidic control liquid drop forming structure and a micro-array capturing structure, wherein all the structures are connected through a channel, the inertia focusing structure, all branch channels of the laminar flow structure and the micro-fluidic liquid drop forming structure respectively have a solution injecting hole, and the micro-array capturing structure has a solution injecting hole and a waste solution outlet. According to the integrated multifunctional controllable cell control and analysis micro-fluidic chip, multiple functional structures are integrated in cell analysis for solving the problem that functions of high-flux analysis, trace analysis, cell controllable manipulation and the like in an existing cell analysis method are difficult to integrate, and the technical field is promoted to be further developed.

Description

technical field [0001] The invention belongs to the technical field of biomedical application of a micro-total analysis system, and in particular relates to a microfluidic chip and its application and development in the field of multifunctional and high-throughput cell analysis systems. Background technique [0002] With the continuous deepening of cell research, from cell groups, cell whole, subcellular structure to molecular structure; from the analysis of components in cells to the analysis of cell respiration, photosynthesis, information transmission, transmembrane transport and other life activities Traditional cell analysis instruments can no longer meet the needs of cell research. In recent years, with the gradual improvement of the theory and technology of microfluidic platforms, especially the establishment of diversified chip manipulation and detection methods, microfluidic chip technology has shown unprecedented vitality in the field of cytology research, mainly r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12Q1/02
Inventor 张何傅昕胡家义
Owner HUNAN INSTITUTE OF ENGINEERING
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