Microsphere particle hydrogel medicine carrier for injection, and preparation method and application thereof

A hydrogel, injection technology, applied in the field of biomedical active materials, can solve problems such as adverse effects, and achieve the effect of excellent fluid adsorption and exchange capacity

Active Publication Date: 2013-10-16
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the former, such as PMMA, has better target drug delivery and PLA is a polymer that can be biodegraded in vivo, such polymers have adverse effects on organisms, such as biocompatibility, longer degradation cycle in vivo, and degradation. The acidic environment produced by the product is not conducive to cell growth, etc.

Method used

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  • Microsphere particle hydrogel medicine carrier for injection, and preparation method and application thereof
  • Microsphere particle hydrogel medicine carrier for injection, and preparation method and application thereof
  • Microsphere particle hydrogel medicine carrier for injection, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) will 0.80×10 5 CFU / ml 1ml of bacterial solution (Acetobacter xylinum, ATCC700178) was inoculated into 100ml of culture solution at a volume ratio of 1:100 (each 100ml of culture solution contained 20g of glucose, 5g of peptone, 2.7g of anhydrous disodium hydrogen phosphate, 1.2g In citric acid, 1 g of magnesium sulfate, 1 g of ammonium sulfate, and 1 mL of corn syrup extract (Sigma, C4648; the same below.)), at 30 ° C and 175 rpm for 3 days, the bacteria cellulose microspheres were removed from the culture solution Take it out, add 0.1mol / L sodium hydroxide solution, shake and break the cells in an 80°C water bath for 5 minutes, then wash with deionized water until the surface pH of the bacterial cellulose microspheres is neutral, and after steam sterilization, 100~ 500 μm bacterial cellulose microspheres; placed in deionized water, stored at 4°C for later use;

[0036] (2) Place the bacterial cellulose microspheres in step (1) in a 0.1mol / L calcium chloride soluti...

Embodiment 2

[0038] (1) Put 1.20×10 5 Inoculate 2ml of CFU / ml bacterial solution (Acetobacter xylinum, ATCC53582) into 100ml of culture solution at a volume ratio of 2:100 (each 100ml of culture solution contains 20g of glucose, 5g of peptone, 2.7g of anhydrous disodium hydrogen phosphate, 1.2g of citric acid, 1g magnesium sulfate, 1g ammonium sulfate and 1mL corn syrup extract), at 28°C, 250rpm rotation culture for 1 day, the bacterial cellulose microspheres were taken out from the culture solution, and 0.1mol / L hydroxide was added Sodium solution, shake and break the cells in a water bath at 80°C for 5 minutes, then wash with deionized water until the surface pH of the bacterial cellulose microspheres is neutral, and after steam sterilization, 100-500 μm bacterial cellulose microspheres are obtained; Store in deionized water at 4°C for later use;

[0039] (2) Place the bacterial cellulose microspheres in step (1) in a 0.05mol / L calcium chloride solution and solidify at room temperature fo...

Embodiment 3

[0041] (1) Convert 2.30×10 5 Inoculate 5ml of CFU / ml bacterial solution (Acetobacter xylinum, ATCC53524) into 100ml of culture solution at a volume ratio of 5:100 (each 100ml of culture solution contains 20g of glucose, 5g of peptone, 2.7g of anhydrous disodium hydrogen phosphate, 1.2g of citric acid, 1g magnesium sulfate, 1g ammonium sulfate and 1mL corn syrup extract), after 5 days of rotation culture at 32°C and 150rpm, the bacterial cellulose microspheres were taken out from the culture solution, and 0.1mol / L hydroxide was added Sodium solution, shake and break the cells in a water bath at 80°C for 5 minutes, then wash with deionized water until the surface pH of the bacterial cellulose microspheres is neutral, and after steam sterilization, 100-500 μm bacterial cellulose microspheres are obtained; Store in deionized water at 4°C for later use;

[0042] (2) Put the bacterial cellulose microspheres in step (1) in a 0.08mol / L calcium chloride solution and solidify at room t...

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Abstract

The invention discloses a microsphere particle hydrogel medicine carrier for injection, and a preparation method and application thereof. The method comprises the following steps: inoculating (0.80-2.30)*105 CFU / ml bacterial solution in culture solution as per the volume ratio of (1:100) to (5:100), carrying out rotary cultivation for 1-5 days at the temperature of 28-32 DEG C to obtain a bacterial cellulose microsphere particle; placing the bacterial cellulose microsphere particle in 0.05-0.1 mol / L calcium chloride solution, rinsing with deionized water after performing room temperature curing for 6-12 hours, and then uniformly mixing the bacterial cellulose microsphere particle and a 10-30 mg / ml dispersing agent as per the massic volume ratio of 1:20 to obtain the microsphere particle hydrogel medicine carrier. The microsphere particle, generated by bacterial cellulose under the rotary fermentation condition, is taken as a host material of the hydrogel medicine carrier, and the bacterial cellulose has excellent fluid adsorption and exchange capability and comparative advantage in medicine loading and releasing as compared with a conventional material, and can be applicable to treatment of diabetes mellitus and obesity.

Description

【Technical field】 [0001] The invention belongs to the technical field of biomedical active materials, and in particular relates to a microsphere particle hydrogel drug carrier for injection and a preparation method and application thereof. 【Background technique】 [0002] In the study of drug delivery combined with tissue engineering, the selection and preparation of materials are very important for constructing an in vivo drug delivery system for tissue repair. An excellent drug-loading system should meet the following requirements: high drug loading capacity; able to help the drug reach the wound site of administration; high fluid exchange capacity for drug release; good biocompatibility. [0003] In recent years, in addition to traditional oral drug delivery systems, injectable drug delivery systems for specific injured sites have become a research hotspot. These drug delivery systems mainly focus on the delivery of drugs in the repair and reconstruction of injured parts ...

Claims

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Application Information

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IPC IPC(8): A61K47/36C12P19/04A61P3/10A61P3/04
Inventor 胡阳潘浩波周新王金慧
Owner SHENZHEN INST OF ADVANCED TECH
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