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A kind of method that utilizes biological treatment to improve the yield of crystalline xylose

A technology for biological treatment and sugar yield, applied in the direction of sugar derivatives, sugar derivatives, sugar derivatives preparation, etc., to achieve the effects of high fermentation purification efficiency, improved crystallization yield, and improved relative content and purity

Active Publication Date: 2016-04-20
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, through searching, there is no report involving the use of biological treatment of hardwood hydrolyzate to increase the yield of crystalline xylose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Treat eucalyptus with high-temperature hot water at 150°C to obtain a treatment solution rich in xylooligosaccharides, concentrate the treatment solution to a concentration of 8% in refractive index, add 0.4% sulfuric acid, and hydrolyze it at 100°C to obtain xylooligosaccharide-rich Hydrolyzate containing xylose. The hydrolyzate is decolorized by adding 10% activated carbon, then neutralized by adding calcium carbonate or milk of lime, and then undergoes steps such as precipitation and filtration to obtain a preliminarily clarified hardwood xylose hydrolyzate. After testing, the glucose content in the hydrolyzate is 3g / L, the xylose is 100g / L, and the galactose is 8g / L. Then add ammonium sulfate 3g / L and yeast powder 1g / L to the hydrolyzate, and then hydrolyze the The solution was sterilized at 115°C for 30 minutes to obtain a fermentation medium;

[0022] (2) Take 100ml of the fermentation medium prepared in step (1), inoculate Saccharomyces cerevisiae CICC310077...

Embodiment 2

[0026] (1) Treat eucalyptus wood with dilute sulfuric acid at 180°C and a solid-to-liquid ratio of 1:8 to obtain a xylose hydrolyzate, concentrate the treated solution to a concentration of 30% of the refractive index, add 30% activated carbon for decolorization, and then Add calcium carbonate or milk of lime for neutralization treatment, and then through steps such as precipitation and filtration, obtain the initially clarified hardwood xylose hydrolyzate; the glucose content in the hydrolyzate is 25g / L, xylose is 120g / L, The galactose is 20g / L, then ammonium sulfate 6g / L and yeast powder 3g / L are added to the hydrolyzate, and then the hydrolyzate is sterilized at 115°C for 30min to obtain a fermentation medium;

[0027] (2) Inoculate Saccharomyces cerevisiae CICC310077 into 50ml of fermentation medium prepared in step (1), and ferment for 18 hours at a temperature of 25°C and a rotation speed of 100rpm, so that the OD value of the fermentation medium detected at 600nm reaches...

Embodiment 3

[0031] (1) Treat birch with high-temperature hot water at 200°C to obtain a treatment solution rich in xylooligosaccharides, concentrate the treatment solution to a concentration of 20% of the refractive index, add 0.5% sulfuric acid, and hydrolyze at 100°C to obtain a treatment solution rich in xylooligosaccharides Hydrolyzate of xylose. Add 25% activated carbon to the hydrolyzate for decolorization treatment, then add calcium carbonate or milk of lime for neutralization treatment, and then undergo steps such as precipitation and filtration to obtain a preliminarily clarified hardwood xylose hydrolyzate. After testing, the glucose content in the hydrolyzate is 15g / L, xylose is 150g / L, and galactose is 20g / L. Add ammonium sulfate 7g / L, yeast powder 7g / L, and then place the hydrolyzate at 115°C Sterilize for 30 minutes to obtain the fermentation medium;

[0032] (2) Inoculate Saccharomyces cerevisiae into the 500ml fermentation medium prepared in step (1), and ferment for 12 h...

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PUM

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Abstract

The invention discloses a method for improving the yield of crystalline xylose by virtue of biological treatment. The method comprises the following steps of: (1) treating hardwood by using high-temperature water in combination with a dilute acid or directly using the dilute acid, thereby obtaining a hydrolysate rich in xylose, and concentrating the hydrolysate until a diopter in the range from 8% to 30%; (2) performing decoloration and neutralization treatment on the hydrolysate, and then realizing initial purification of the hydrolysate through the steps of settling and filtering; (3) adding nutrient substances to the hydrolysate to form a fermentation culture medium; (4) inoculating a wine-making yeast to the formed fermentation culture medium, thereby obtaining a yeast seed solution; (5) inoculating the seed solution to the fermentation culture medium for fermentation, and purifying the fermentation liquor after fermentation is completed; (6) separating out xylose crystals from the purified fermentation liquor through the steps of concentration, decoloration, ion exchange and concentrating crystallization. The method provided by the invention has the advantage that heteroside components in the hydrolysate are eliminated so that the purity of the xylose and the yield of the crystalline xylose in the hydrolysate are effectively improved; the method is low in cost, simple to operate and suitable for industrial popularization.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for improving the yield of crystallized xylose by biological treatment. Background technique [0002] Xylose is a kind of pentose sugar, the appearance is white crystal, the sweetness is about 70% of sucrose, the sweetness is close to that of glucose, and the flavor is also similar to glucose. Xylitol can be produced from xylose after hydrogenation. Xylose and xylitol are important chemical products and raw materials. They can be used as sweeteners in the production of functional foods to meet the sugar needs of diabetic patients; they can also be used as chemical raw materials for the preparation of surfactants, emulsifying Agents, demulsifiers, alkyd resins and coatings, and also used as raw materials for the manufacture of various drugs in the pharmaceutical industry. At present, xylose and xylitol have been widely used in many industries such as food, health care product...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/02C07H1/06
Inventor 李雪芝赵建
Owner SHANDONG UNIV