Total-reflection microscope circular scanning method and device based on digital micro-mirror device

A technology of digital micromirror element and scanning device, applied in microscopes, measuring devices, optical elements, etc., can solve the problems of complex design of scanning modules, complex output waveforms, insufficient versatility, etc., and achieve fast scanning speed and strong system applicability. , Design flexible effects

Active Publication Date: 2013-10-23
ZHEJIANG UNIV
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This approach has extremely high requirements for the control circuit of the two-dimensional galvanometer group, not only requires it to have a high frequency and response speed, but also its output waveform is very complex
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  • Total-reflection microscope circular scanning method and device based on digital micro-mirror device
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  • Total-reflection microscope circular scanning method and device based on digital micro-mirror device

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Embodiment Construction

[0033] The content of the present invention will be specifically described below with reference to the accompanying drawings, but the present invention is not limited thereto.

[0034] figure 1 It is the system schematic diagram of the total reflection microscope circular scanning device based on the digital micromirror element of the present invention, including:

[0035] A laser light source 1, a polarization converter 2, a coupling lens group 3, a square rod 4, an illumination lens 5, a digital micromirror element 6, an imaging lens 7, and a total internal reflection microscope objective lens 8. All optical components are on the coaxial optical path.

[0036] The excitation light emitted from the laser light source 1 is converted into circularly polarized light by the polarization converter 2 . Wherein, the wavelength and output power of the laser light source 1 are determined by the properties of the fluorescent dye used to mark the sample 9 . Polarization converter 2 h...

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Abstract

The invention discloses a total-reflection microscope circular scanning method based on a digital micro-mirror device. The method comprises the following steps of: establishing a circular image scanning sequence on the digital micro-mirror device; performing uniform beam-expanding treatment on exciting light and allowing the exciting light to enter the digital micro-mirror device, and reflecting the exciting light and irradiating the exciting light to a sample through a total internal reflection microobjective; and finishing circular scanning of the sample according to the circular image scanning sequence. The invention also discloses a total-reflection microscope circular scanning device based on the digital micro-mirror device. The scanning device comprises a laser light source, a polarization converter, a coupling lens group, a square rod, an illuminating lens, a digital micro-mirror device, an imaging lens and a total internal reflection microobjective which are sequentially arranged along a light path. The device is simple in structure, high in scanning speed and high in system applicability and can be suitable for the condition that the exciting light has optional wavelengths.

Description

technical field [0001] The invention belongs to the field of microscopic observation and measurement, and in particular relates to a ring scanning method and device for a total reflection microscope based on a digital micromirror element. Background technique [0002] A microscope generally refers to a tool that can magnify the image of a tiny invisible or hard-to-see object so that it can be observed by the naked eye or other imaging instruments. As the earliest and largest branch of the microscope family, optical microscopes are usually composed of several lenses or lens groups plus necessary mechanical structures, which play a very important role in promoting the development of biology, medicine, materials and other fields. With the advancement of science and technology, optical microscopes are becoming more and more specialized, and special microscope products and technologies for some special application fields have emerged. Among these products, total internal reflect...

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Application Information

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IPC IPC(8): G01N21/01G02B21/06G02B26/10G01N21/64
Inventor 匡翠方郝翔李旸晖刘旭
Owner ZHEJIANG UNIV
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