Microsatellite loci of portunus trituberculatus and primer
A technology of microsatellite loci and Portunus trituratus, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of restricted development, low degree of improved breeding, etc., and achieves high polymorphism Effects of Sex and Stability
Active Publication Date: 2013-11-13
NINGBO UNIV
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But at present, the crab seedlings of Portunus trituberculatus aquaculture mainly rely on wild resources, and hav
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[0019] The method of the present invention will be described in detail below in conjunction with specific examples. For experimental methods that do not indicate specific conditions in the examples, conventional conditions can usually be used, such as the "Molecular Cloning Experiment Guide" written by J. Sambrook (Sambrook) et al. 》, or run under the conditions recommended by the manufacturer.
[0020] 1. Screening of microsatellite sites
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The invention aims at providing microsatellite loci of portunus trituberculatus and a polymorphic primer, namely providing five microsatellite loci of portunus trituberculatus. A nucleotide sequence of the microsatellite loci is any one of SEQ ID NO:1 to 5. The invention also provides a primer designed from the microsatellite loci. The primer is used for genetic diversity detection of portunus trituberculatus population. By adopting the microsatellite loci of portunus trituberculatus, five microsatellite loci are screened from a public database of the portunus trituberculatus; a specific primer is designed according to flanking sequences at two ends of the microsatellite; the amplification result has high polymorphism and stability. The primer can be applied to the fields such as genetic diversity detection and relationship identification of the portunus trituberculatus population, building of a genetic linkage map, and assistant breeding of a molecular marker.
Description
technical field [0001] The invention belongs to the technical field of molecular biology DNA markers, and in particular relates to a microsatellite site and a primer of Portunus trituberculatus. Background technique [0002] Portunus trituberculatus (Portunus trituberculatus) referred to as Portunus, commonly known as river crab, white crab, belongs to Crustacea, Decapoda, Portunidae, Portunus, is a large marine crab . It is widely distributed in the sea areas from Liaodong Peninsula and Shandong Peninsula in the north of my country to Guangdong and Guangxi in the south, as well as the waters of Japan, Korea, and the Malaysian archipelago. Portunus trituberculatus is suitable for mariculture in coastal ponds due to its fast growth, large individual size and delicious meat taste. Therefore, it has gradually developed as a mariculture object and has become an important fishery catch and mariculture object in my country. But at present, the crab seedlings of Portunus trituber...
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Inventor 魏倩王春琳李荣华母昌考宋微微
Owner NINGBO UNIV
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