Microsatellite loci of portunus trituberculatus and primer

Abstract

The invention aims at providing microsatellite loci of portunus trituberculatus and a polymorphic primer, namely providing five microsatellite loci of portunus trituberculatus. A nucleotide sequence of the microsatellite loci is any one of SEQ ID NO:1 to 5. The invention also provides a primer designed from the microsatellite loci. The primer is used for genetic diversity detection of portunus trituberculatus population. By adopting the microsatellite loci of portunus trituberculatus, five microsatellite loci are screened from a public database of the portunus trituberculatus; a specific primer is designed according to flanking sequences at two ends of the microsatellite; the amplification result has high polymorphism and stability. The primer can be applied to the fields such as genetic diversity detection and relationship identification of the portunus trituberculatus population, building of a genetic linkage map, and assistant breeding of a molecular marker.

Description

technical field [0001] The invention belongs to the technical field of molecular biology DNA markers, and in particular relates to a microsatellite site and a primer of Portunus trituberculatus. Background technique [0002] Portunus trituberculatus (Portunus trituberculatus) referred to as Portunus, commonly known as river crab, white crab, belongs to Crustacea, Decapoda, Portunidae, Portunus, is a large marine crab . It is widely distributed in the sea areas from Liaodong Peninsula and Shandong Peninsula in the north of my country to Guangdong and Guangxi in the south, as well as the waters of Japan, Korea, and the Malaysian archipelago. Portunus trituberculatus is suitable for mariculture in coastal ponds due to its fast growth, large individual size and delicious meat taste. Therefore, it has gradually developed as a mariculture object and has become an important fishery catch and mariculture object in my country. But at present, the crab seedlings of Portunus trituber...

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Problems solved by technology

But at present, the crab seedlings of Portunus trituberculatus aquaculture mainly rely on wild resources, and have hardly been artificially selected, and the degree of improved species is not high, which limits its development.

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