Tremella aurantialba culture medium and culture preparation method

A technology for culture medium preparation and Auricularia species, which is applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the difficulty of artificial cultivation and promotion of Auricularia, there is no key breakthrough in Auricularia, and there is no germination fungus. Golden ear and other problems, achieve the effect of shortening the seed production cycle, avoiding the use of pesticides, and growing rapidly

Active Publication Date: 2013-11-20
昆明云蕈科技开发有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, in the artificial cultivation and production of golden ear, the problem of golden ear seed production is that its associated pili are dominant, while the golden fungus mycelium is weak, so that the ear yield rate of golden ear cultivated by section wood or bag material is low, The problem of poor quality, or even the problem of only germs and no golden ears, makes it difficult to promote artificial cultivation of golden ears, especially the bag cultivation of golden ears has not had a key breakthrough in the past 30 years

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Components of culture medium for golden ear bacteria and preparation method of bacteria

[0033] The composition of golden ear culture medium is 89% by weight of buckwheat seeds, 10% of tung seed shells, and 1% of superphosphate. The following is the preparation method of the bacteria:

[0034] A. Preparation of the first-generation test tube parent species

[0035] Use PDA medium to add bran juice with a volume ratio of 3% to make a test tube medium. Select the golden ear fruiting body with large flowers, golden color and no pollution. After disinfection, inoculate under sterile conditions, and place it at temperature Cultivate for 15 days at 15°C and 50% humidity, and when the seed ear is swollen to 3cm, it will be used as the first-generation test tube mother seed of golden ear;

[0036] B. Preparation of grain seed

[0037]Choose buckwheat seeds with plump grains and no impurities as the main material, which weighs 89kg, soak in water for 48 hours, filter out...

Embodiment 2

[0043] (1) Components of culture medium for golden ear bacteria and preparation method of bacteria

[0044] The composition of golden ear culture medium is 90% by weight of wheat grain, 9% of rubber seed husk, and 1% of light calcium carbonate. The following is the preparation method of the bacteria:

[0045] A. Preparation of the first-generation test tube parent species

[0046] Use PDA medium to add broad-leaved bark juice with a volume ratio of 4% to make a test tube medium, select large, golden color, and non-polluting golden ear fruiting bodies, disinfect them, inoculate them under sterile conditions, and place them at temperature Cultivate for 14 days at 17°C with a humidity of 53%, and when the ears are swollen to 2cm, they will be used as the first-generation test tube mother species of golden ears;

[0047] B. Preparation of grain seed

[0048] Choose wheat grains with full grains and no impurities as the main material, which weighs 90kg, soak in water for 50 hour...

Embodiment 3

[0054] (1) Components of culture medium for golden ear bacteria and preparation method of bacteria

[0055] The composition of golden ear culture medium is 91% by weight of sorghum seeds, 8% of camellia seed shells, and 1% of potassium dihydrogen phosphate. The following is the preparation method of the bacteria:

[0056] A. Preparation of the first-generation test tube parent species

[0057] Use PDA medium to add bran infusion with a volume ratio of 5% to make a test tube medium, select large, golden color, and non-polluting golden fungus fruiting bodies, disinfect them, inoculate them under sterile conditions, and place them at temperature Cultivate for 13 days at 19°C and 55% humidity, and when the seed ears are swollen to 1cm, they will be used as the first-generation golden ear test tube parent species;

[0058] B. Preparation of grain seed

[0059] Choose sorghum seeds with full grains and no impurities as the main ingredient, weighing 91kg, soak them in water for 51...

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PUM

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Abstract

The invention discloses a tremella aurantialba culture medium and a culture preparation method. The tremella aurantialba culture medium comprises main materials, auxiliary materials and a few nutrient elements, wherein the main materials are grain seeds with a weight ratio of 89-94%, and include any one or more combined seeds of buckwheat, sorghum, corn and rice seeds; the auxiliary materials are biodiesel leftovers or bacterium residues with a weight ratio of 5-10%, and include copper seed husks, rubber seed husks and oil tea seed husks; the nutrient elements are calcium, phosphorus, sulfur and potassium; and a weight ratio of a calcium compound, a phosphorus compound, a sulfur compound or a potassium compound is 1%. The tremella aurantialba culture preparation method based on the medium comprises the steps of preparing a first test tube stock culture, a grain seed stock culture, an original grain seed culture and a cultivated culture. The tremella aurantialba culture medium and the culture preparation method have the characteristics that tremella aurantialba hyphae are high in material absorption, a tremella aurantialba emergence rate is as high as 100%, the whole production cycle is only 60d, and the output growth of tremella aurantialba can be above 30%, so that a requirement of the large-scale cultivation culture of a tremella aurantialba bag material is met.

Description

technical field [0001] The invention belongs to the technical field of wild edible fungus cultivation, and in particular relates to a golden ear fungus culture medium and a method for preparing the fungus. Background technique [0002] Golden ear (Tremella aurantialba Bandoni & Zang.sp.nor.) belongs to Microbial Kingdom, Fungal Phylum, Basidiomycotina, Basidiomycetes, Heteroepia Basidiomycetes, Tremellales, Tremellaceae, Tremella genus. Golden ear has another name called orange-yellow white fungus and brain ear. Its fruiting body is golden yellow, the smell is delicate, and the taste is sticky and delicious. Modern scientific research has proved that golden ear is rich in mucopolysaccharides, carotenoids, proteins, amino acids, various trace elements, etc. and unique golden ear element. Growth; regulate the body's metabolic function, improve the body's nutritional status, increase the body's hemoglobin and plasma content; improve the body's anti-aging and anti-hypoxia abili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00A01G1/04
Inventor 罗星野陈天蓉申洁申帆
Owner 昆明云蕈科技开发有限责任公司
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