A kind of golden ear fungus culture medium and the method for strain preparation
A technology for the preparation of culture medium and strains of golden ear strains, which is applied to fungi and other directions, can solve the problems of difficulty in artificial cultivation and popularization of golden ears, no key breakthroughs in golden ears, and no golden ears for leather-producing bacteria, so as to shorten the production time. Plant cycle, avoid the use of pesticides, the effect of rapid growth
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Embodiment 1
[0032] (1) Components of culture medium for golden ear bacteria and preparation method of bacteria
[0033] The composition of golden ear culture medium is 89% by weight of buckwheat seeds, 10% of tung seed shells, and 1% of superphosphate. The following is the preparation method of the bacteria:
[0034] A. Preparation of the first-generation test tube parent species
[0035] Use PDA medium to add bran juice with a volume ratio of 3% to make a test tube medium. Select the golden ear fruiting body with large flowers, golden color and no pollution. After disinfection, inoculate under sterile conditions, and place it at temperature Cultivate for 15 days at 15°C and 50% humidity, and when the seed ear is swollen to 3cm, it will be used as the first-generation test tube mother seed of golden ear;
[0036] B. Preparation of grain seed
[0037]Choose buckwheat seeds with plump grains and no impurities as the main material, which weighs 89kg, soak in water for 48 hours, filter out...
Embodiment 2
[0043] (1) Components of culture medium for golden ear bacteria and preparation method of bacteria
[0044] The composition of golden ear culture medium is 90% by weight of wheat grain, 9% of rubber seed husk, and 1% of light calcium carbonate. The following is the preparation method of the bacteria:
[0045] A. Preparation of the first-generation test tube parent species
[0046] Use PDA medium to add broad-leaved bark juice with a volume ratio of 4% to make a test tube medium, select large, golden color, and non-polluting golden ear fruiting bodies, disinfect them, inoculate them under sterile conditions, and place them at temperature Cultivate for 14 days at 17°C with a humidity of 53%, and when the ears are swollen to 2cm, they will be used as the first-generation test tube mother species of golden ears;
[0047] B. Preparation of grain seed
[0048] Choose wheat grains with full grains and no impurities as the main material, which weighs 90kg, soak in water for 50 hour...
Embodiment 3
[0054] (1) Components of culture medium for golden ear bacteria and preparation method of bacteria
[0055] The composition of golden ear culture medium is 91% by weight of sorghum seeds, 8% of camellia seed shells, and 1% of potassium dihydrogen phosphate. The following is the preparation method of the bacteria:
[0056] A. Preparation of the first-generation test tube parent species
[0057] Use PDA medium to add bran infusion with a volume ratio of 5% to make a test tube medium, select large, golden color, and non-polluting golden fungus fruiting bodies, disinfect them, inoculate them under sterile conditions, and place them at temperature Cultivate for 13 days at 19°C and 55% humidity, and when the seed ears are swollen to 1cm, they will be used as the first-generation golden ear test tube parent species;
[0058] B. Preparation of grain seed
[0059] Choose sorghum seeds with full grains and no impurities as the main ingredient, weighing 91kg, soak them in water for 51...
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