Processes for treating textile with polypeptide having cellulolytic enzyme enhancing activity
A technology for textiles and cellulase, which can be used in fiber processing, biochemical fiber processing, textiles and papermaking, etc., and can solve problems such as no abrasive effect.
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Embodiment 1
[0184] Example 1: Denim Abrasion with Cellulase and Ta GH61 in a Launder-O-meter
[0185] In Launder-O-Meter (SDL-Atlas LP2), the mature polypeptide of Ta GH61 was tested against Cellusoft Neupolish The effects of abrasive denim, including abrasion, backstain, and treatment bath color.
[0186] Raw denim was desized and cut to 12.5 cm high and 23 cm long. The denim was cut and sewn to form a tube 12.5 cm high and weighing approximately 14 g. The tubes were placed in a conditioned room (65% relative humidity, 20°C) for 24 hours, after which they were numbered, weighed with an analytical balance, and recorded. Place one conditioned tube in each 500ml beaker, blue side inward. For each beaker, use 30 large nuts (M10M6M-SR-A4-80, acid resistant), 10 small nuts (M6M6M-SR-A4-80, acid resistant), 7 large star magnets (diameter 17mm , Product No. 3-CO-411117, Cowie, Schweiz via Bie & Berntsen), and 3 small star magnets (diameter 14 mm, Product No. 3-CO-11117, Cowie, Schweiz via Bie...
Embodiment 2
[0194] Example 2: Denim Abrasion in LOM with Cellulase and Added Af GH61
[0195] Also tested the mature polypeptide of Af GH61 to Cellusoft Neupolish with the same experimental scheme as embodiment 1 The effect of abrasive denim. The processing time here is 2 hours.
[0196] As shown in Table 2, it was found that with 0.016mg enzyme protein / g fabric from Cellusoft Neupolish Addition of 0.032mg Af GH61 / g fabric together with the cellulase enhanced abrasion, which was shown by the increase of ΔL* from 7.1 to 8.1 with the addition of Af GH61. And Af GH61 also made the bath more bluish, indicating an increased release of the dye from the fabric to the supernatant, which manifested itself as a higher OD590 value in solution.
[0197] Table 2: Results of denim abrasion in LOM at 55°C, pH 6.5, 2 hours
[0198]
[0199] Note: Average of three samples for each enzyme combination
Embodiment 3
[0200] Example 3: Denim abrasion with cellulase, Ta GH61 and L-cysteine in LOM
[0201] Test L-cysteine against Cellusoft Neupolish in LOM and Cellusoft Neupolish Abrasion of denim by a mixture of Ta / Ta GH61 mature polypeptides. Test condition is identical with embodiment 1, just 0-20mM L-cysteine and Cellusoft Neupolish or Cellusoft Neupolish / Ta GH61 mixture was added together. In this example, the treatment time was set to 0.5 hours.
[0202] As shown in Table 3, it was confirmed that the enzyme protein from Cellusoft Neupolish with 0.05mg enzyme protein / g fabric Adding 0.05mg Ta GH61 / g fabric together with cellulase can improve abrasion from 1.14 to 1.59, and reduce back staining level from -1.54 to -1.36. The bath also became more bluish with the addition of Ta GH61. And in Cellusoft Neupolish A further addition of 5 mM L-cysteine as an auxiliary substance in / Ta GH61 enhanced abrasion from 1.59 to a higher level of 1.80 and reduced restaining from -...
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