A kind of preparation method of total diterpene extract of quasi-fragrance-deficient tea vegetables

A technology for imitating tea vegetables lacking in fragrance and total diterpenes, which is applied in the field of medicine to achieve great economic and social benefits, simple method and good quality

Inactive Publication Date: 2015-08-12
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a preparation method of the total diterpene extract of pseudo-fragrant tea vegetables, which can effectively solve the problem of extracting total diterpenes from pseudo-fragrant tea vegetables with high quality and high extraction rate

Method used

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  • A kind of preparation method of total diterpene extract of quasi-fragrance-deficient tea vegetables
  • A kind of preparation method of total diterpene extract of quasi-fragrance-deficient tea vegetables
  • A kind of preparation method of total diterpene extract of quasi-fragrance-deficient tea vegetables

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Embodiment 1

[0020] In concrete implementation, the present invention is realized by the following steps:

[0021] (1) Preparation of the sample solution: crush 1kg of the dried medicinal materials to be lacking in fragrant tea, add water to decoct twice, each time for 1 hour, add 12L of water for the first time, add 8L of water to the medicinal residue for the second time, Combine the two water decoctions, filter, let stand and cool to room temperature, and obtain 10L of supernatant, which is the sample solution;

[0022] (2) Resin pretreatment: The method is to soak the D101 macroporous adsorption resin in ethanol with a volume concentration of 95% for 24 hours, then wet-pack the column, and dynamically clean the resin with ethanol with a volume concentration of 95%. 5 times the volume of the resin, washed with water until there is no alcohol smell, soaked in hydrochloric acid with a volume concentration of 5% for 6 hours, and dynamically cleaned the resin. The amount of hydrochloric aci...

Embodiment 2

[0026] The present invention is realized by the following steps:

[0027] (1) Preparation of the sample solution: crush 1kg of the dried medicinal materials to be lacking in fragrant tea, add water to decoct twice, each time for 1 hour, add 12L of water for the first time, add 8L of water to the medicinal residue for the second time, Combine the two water decoctions, filter, let stand and cool to room temperature, and obtain 10L of supernatant, which is the sample solution;

[0028] (2) Resin pretreatment: The method is to soak the HP20 macroporous adsorption resin with ethanol with a volume concentration of 95% for 24 hours, then wet-pack the column, and use ethanol with a volume concentration of 95% to dynamically clean the resin. The amount of ethanol added 5 times the volume of the resin, washed with water until there is no alcohol smell, soaked in hydrochloric acid with a volume concentration of 5% for 6 hours, and dynamically cleaned the resin. The amount of hydrochloric...

Embodiment 3

[0032] The present invention is realized by the following steps:

[0033] (1) Preparation of the sample solution: crush 1kg of the dried medicinal materials to be lacking in fragrant tea, add water to decoct twice, each time for 1 hour, add 12L of water for the first time, add 8L of water to the medicinal residue for the second time, Combine the two water decoctions, filter, let stand and cool to room temperature, and obtain 10L of supernatant, which is the sample solution;

[0034] (2) Resin pretreatment: The method is to soak the AB8 macroporous adsorption resin in ethanol with a volume concentration of 95% for 24 hours, then wet-pack the column, and use ethanol with a volume concentration of 95% to dynamically clean the resin. The amount of ethanol added 5 times the volume of the resin, washed with water until there is no alcohol smell, soaked in hydrochloric acid with a volume concentration of 5% for 6 hours, and dynamically cleaned the resin. The amount of hydrochloric ac...

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Abstract

The invention relates to a preparation method of an isodon excisoides total diterpenoid extractive, which is capable of effectively solving the problem of extracting total diterpenoid from isodon excisoides with high quality and high extraction rate. The preparation method disclosed by the invention comprises the following steps of crushing isodon excisoides, decocting by adding water, filtering water decoction, and cooling to form sampling solution; after immersing macroreticular resin by using ethanol, dynamically cleaning resin by using ethanol, washing till alcohol smell does not exist, immersing by using hydrochloric acid, washing again till the macroreticular resin is neutral, then, immersing by using NaOH solution, dynamically cleaning resin, washing till the macroreticular resin is neutral, after dynamically eluting ethanol, packing the macroreticular resin in a column through a wet method, and dynamically absorbing the sampling solution through a resin column; and eluting the sample column by using water after absorbing, eluting through ethanol again, collecting ethanol eluent, decompressing and recycling the solvent, and evaporating, thereby obtaining the isodon excisoides total diterpenoid extractive. The preparation method disclosed by the invention is simple, easy to operate and use, steady, reliable, high in product yield, good in quality, simple, practical and free from pollution; research basis is provided for researching isodon amethystoides diterpenoid anti-tumour candidate drugs.

Description

technical field [0001] The invention relates to a total diterpene extract of pseudo-fragrant tea vegetables and a preparation method of a macroporous adsorption resin pseudo-fragrant total diterpene extract of tea vegetables, belonging to the field of medicine. Background technique [0002] The plants of the genus Camellia show obvious anti-tumor effects. Since 1976, the diterpenoids in the plants of this genus have become the focus of domestic and foreign scholars seeking new anti-tumor lead compounds. In recent years, with the successive discoveries of new caraway diterpenoids, a large number of cytotoxic diterpenoid components have been reported. At present, as anti-tumor candidate compounds, there are oridonin and echinacein A. , Big-leaf Fragrant Tea Vegetable Geng Su, etc., related products Sanmei Tablets (fine-leaf Fragrant Tea Vegetables), Donglingcao Syrup, Tablets (Donglingcao), Xiaoyan Lidan Tablets (containing Xihuangcao), etc. In addition, it has been reported ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/00A61K31/01
Inventor 代丽萍杨晶凡陈随清鲁艳清王智民廉伟伟刘丹
Owner HENAN UNIV OF CHINESE MEDICINE
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